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Mitochondrial-targeted catalase is good for the old mouse proteome, but not for the young: 'reverse' antagonistic pleiotropy?

Basisty N, Dai DF, Gagnidze A, Gitari L, Fredrickson J, Maina Y, Beyer RP, Emond MJ, Hsieh EJ, MacCoss MJ, Martin GM, Rabinovitch PS - Aging Cell (2016)

Bottom Line: However, it has been increasingly recognized that ROS also has beneficial roles in signaling, hormesis, stress response, and immunity.Protein abundance of old mCAT hearts recapitulated a more youthful proteomic expression profile (P-value < 0.01).Age strongly interacts with mCAT, consistent with antagonistic pleiotropy in the reverse of the typical direction.

View Article: PubMed Central - PubMed

Affiliation: Department of Pathology, University of Washington, 1959 NE Pacific Ave, Seattle, WA, 98195, USA.

No MeSH data available.


Related in: MedlinePlus

Hierarchical heatmap of all cardiac proteins significantly changed in half‐life during aging and correlation plots of cardiac proteins that significantly changed with age. (A) Heatmap depicting all proteins with significantly altered (P‐value < 0.05) in the statistical comparison of YWT and OWT proteome half‐lives. The four columns correspond to the treatment groups used in this study: YWT, OWT, YmCAT, and OmCAT. Colors in the heatmap represent the half‐life of a protein relative to its mean half‐life (in days) across all treatment groups (difference from row mean). Correlations were made between OWT half‐lives vs. each treatment group—(B) YWT, (C) OmCAT, and (D) YmCAT. (E) A correlation of WT aging (OWT/YWT) vs. mCAT aging (OmCAT/YmCAT). *P‐value < 0.05, **P‐value < 0.001 for Spearman's correlation of the individual pathways in the regressions.
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acel12472-fig-0002: Hierarchical heatmap of all cardiac proteins significantly changed in half‐life during aging and correlation plots of cardiac proteins that significantly changed with age. (A) Heatmap depicting all proteins with significantly altered (P‐value < 0.05) in the statistical comparison of YWT and OWT proteome half‐lives. The four columns correspond to the treatment groups used in this study: YWT, OWT, YmCAT, and OmCAT. Colors in the heatmap represent the half‐life of a protein relative to its mean half‐life (in days) across all treatment groups (difference from row mean). Correlations were made between OWT half‐lives vs. each treatment group—(B) YWT, (C) OmCAT, and (D) YmCAT. (E) A correlation of WT aging (OWT/YWT) vs. mCAT aging (OmCAT/YmCAT). *P‐value < 0.05, **P‐value < 0.001 for Spearman's correlation of the individual pathways in the regressions.

Mentions: Proteins that showed a significant age or mCAT‐dependent change in HL (P‐value < 0.05) were plotted on a heatmap with hierarchical clustering in both rows and columns (Fig. 2A). The first two columns, showing mostly decreases (blue) in half‐life of OWT and YmCAT compared to the row means, illustrate the visual similarity between aged WT and young mCAT mice. This is in contrast to the increasing half‐lives (red) seen in a higher proportion of proteins in the third and fourth columns, which include old mCAT and young WT mice.


Mitochondrial-targeted catalase is good for the old mouse proteome, but not for the young: 'reverse' antagonistic pleiotropy?

Basisty N, Dai DF, Gagnidze A, Gitari L, Fredrickson J, Maina Y, Beyer RP, Emond MJ, Hsieh EJ, MacCoss MJ, Martin GM, Rabinovitch PS - Aging Cell (2016)

Hierarchical heatmap of all cardiac proteins significantly changed in half‐life during aging and correlation plots of cardiac proteins that significantly changed with age. (A) Heatmap depicting all proteins with significantly altered (P‐value < 0.05) in the statistical comparison of YWT and OWT proteome half‐lives. The four columns correspond to the treatment groups used in this study: YWT, OWT, YmCAT, and OmCAT. Colors in the heatmap represent the half‐life of a protein relative to its mean half‐life (in days) across all treatment groups (difference from row mean). Correlations were made between OWT half‐lives vs. each treatment group—(B) YWT, (C) OmCAT, and (D) YmCAT. (E) A correlation of WT aging (OWT/YWT) vs. mCAT aging (OmCAT/YmCAT). *P‐value < 0.05, **P‐value < 0.001 for Spearman's correlation of the individual pathways in the regressions.
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Related In: Results  -  Collection

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getmorefigures.php?uid=PMC4933659&req=5

acel12472-fig-0002: Hierarchical heatmap of all cardiac proteins significantly changed in half‐life during aging and correlation plots of cardiac proteins that significantly changed with age. (A) Heatmap depicting all proteins with significantly altered (P‐value < 0.05) in the statistical comparison of YWT and OWT proteome half‐lives. The four columns correspond to the treatment groups used in this study: YWT, OWT, YmCAT, and OmCAT. Colors in the heatmap represent the half‐life of a protein relative to its mean half‐life (in days) across all treatment groups (difference from row mean). Correlations were made between OWT half‐lives vs. each treatment group—(B) YWT, (C) OmCAT, and (D) YmCAT. (E) A correlation of WT aging (OWT/YWT) vs. mCAT aging (OmCAT/YmCAT). *P‐value < 0.05, **P‐value < 0.001 for Spearman's correlation of the individual pathways in the regressions.
Mentions: Proteins that showed a significant age or mCAT‐dependent change in HL (P‐value < 0.05) were plotted on a heatmap with hierarchical clustering in both rows and columns (Fig. 2A). The first two columns, showing mostly decreases (blue) in half‐life of OWT and YmCAT compared to the row means, illustrate the visual similarity between aged WT and young mCAT mice. This is in contrast to the increasing half‐lives (red) seen in a higher proportion of proteins in the third and fourth columns, which include old mCAT and young WT mice.

Bottom Line: However, it has been increasingly recognized that ROS also has beneficial roles in signaling, hormesis, stress response, and immunity.Protein abundance of old mCAT hearts recapitulated a more youthful proteomic expression profile (P-value < 0.01).Age strongly interacts with mCAT, consistent with antagonistic pleiotropy in the reverse of the typical direction.

View Article: PubMed Central - PubMed

Affiliation: Department of Pathology, University of Washington, 1959 NE Pacific Ave, Seattle, WA, 98195, USA.

No MeSH data available.


Related in: MedlinePlus