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Anticonvulsant effect of Satureja hortensis aerial parts extracts in mice.

Zolfagharian F, Razavi BM, Hosseinzadeh H - Avicenna J Phytomed (2016 May-Jun)

Bottom Line: Regarding the anticonvulsant effects of Satureja hortensis (S . hortensis) in Avicenna's book: canon of medicine; the present study was undertaken to evaluate the anti- eplileptic effects of S . hortensis aqueous and ethanolic aerial part extracts.Aqueous and ethanolic extracts (400 and 600 mg/kg) significantly increased MCS and GTCS latencies in PTZ model.S. hortensis showed anticonvulsant activity in PTZ model and this effect may be mediated, at least partly, through interacting with nitric oxide and GABAA-BZD receptor complex.

View Article: PubMed Central - PubMed

Affiliation: School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran.

ABSTRACT

Objective: Regarding the anticonvulsant effects of Satureja hortensis (S . hortensis) in Avicenna's book: canon of medicine; the present study was undertaken to evaluate the anti- eplileptic effects of S . hortensis aqueous and ethanolic aerial part extracts. Furthermore, the mechanisms of their anticonvulsant activities were also evaluated.

Materials and methods: Seizure was induced by Pentylentetrazol (PTZ) and MES (maximal electroshock) models. Mice were randomly divided into 8 groups; negative control (normal saline, 10ml/Kg), positive control (diazepam, 2 mg/kg), S. hortensis aqueous and ethanolic extracts (200, 400 and 600 mg/kg). In PTZ test, latency to the first minimal clonic seizure (MCS), latency to the first generalized tonic-clonic seizures (GTCS), the total duration of seizures and protection against mortality were evaluated. In MES test, the stretching length of extremities and protection against mortality were recorded.

Results: Aqueous and ethanolic extracts (400 and 600 mg/kg) significantly increased MCS and GTCS latencies in PTZ model. Three doses of the extracts decreased the total duration of seizure. These extracts did not show any protective effects on seizure induced by MES model. In PTZ model, flumazenil, an antagonist of benzodiazepine (BZD) site in the GABAA-BZD receptor complex and 7- nitroindazole (7- NI), a selective nNOS (neuronal nitric oxide synthase) inhibitor, reduced the prolongation of seizure latency.

Conclusion: S. hortensis showed anticonvulsant activity in PTZ model and this effect may be mediated, at least partly, through interacting with nitric oxide and GABAA-BZD receptor complex.

No MeSH data available.


Related in: MedlinePlus

Effect of aqueous and ethanolic extracts of S. hortensis on the latency to first generalized tonic-clonic seizure (GTCS) in PTZ-induced seizure in the presence and absence of flumazenil in mice. Data presented as mean ± SEM. Tukey Kramer, ***p<00.1 vs normal saline, +++ p<0.001 vs extracts received flumazenil. n=6. (ns= Normal saline , ex= Extract , flu= Flumazenil
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Figure 8: Effect of aqueous and ethanolic extracts of S. hortensis on the latency to first generalized tonic-clonic seizure (GTCS) in PTZ-induced seizure in the presence and absence of flumazenil in mice. Data presented as mean ± SEM. Tukey Kramer, ***p<00.1 vs normal saline, +++ p<0.001 vs extracts received flumazenil. n=6. (ns= Normal saline , ex= Extract , flu= Flumazenil

Mentions: Flumazenil reduced the anticonvulsant activity of the aqueous and ethanolic extracts (600 mg/kg) by decreasing the latency to the first minimal clonic seizure (MCS) (p<0.001) (Figure 7) and the latency to the first generalized tonic–clonic seizures (GTCS) (p<0.001) (Figure 8).


Anticonvulsant effect of Satureja hortensis aerial parts extracts in mice.

Zolfagharian F, Razavi BM, Hosseinzadeh H - Avicenna J Phytomed (2016 May-Jun)

Effect of aqueous and ethanolic extracts of S. hortensis on the latency to first generalized tonic-clonic seizure (GTCS) in PTZ-induced seizure in the presence and absence of flumazenil in mice. Data presented as mean ± SEM. Tukey Kramer, ***p<00.1 vs normal saline, +++ p<0.001 vs extracts received flumazenil. n=6. (ns= Normal saline , ex= Extract , flu= Flumazenil
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4930537&req=5

Figure 8: Effect of aqueous and ethanolic extracts of S. hortensis on the latency to first generalized tonic-clonic seizure (GTCS) in PTZ-induced seizure in the presence and absence of flumazenil in mice. Data presented as mean ± SEM. Tukey Kramer, ***p<00.1 vs normal saline, +++ p<0.001 vs extracts received flumazenil. n=6. (ns= Normal saline , ex= Extract , flu= Flumazenil
Mentions: Flumazenil reduced the anticonvulsant activity of the aqueous and ethanolic extracts (600 mg/kg) by decreasing the latency to the first minimal clonic seizure (MCS) (p<0.001) (Figure 7) and the latency to the first generalized tonic–clonic seizures (GTCS) (p<0.001) (Figure 8).

Bottom Line: Regarding the anticonvulsant effects of Satureja hortensis (S . hortensis) in Avicenna's book: canon of medicine; the present study was undertaken to evaluate the anti- eplileptic effects of S . hortensis aqueous and ethanolic aerial part extracts.Aqueous and ethanolic extracts (400 and 600 mg/kg) significantly increased MCS and GTCS latencies in PTZ model.S. hortensis showed anticonvulsant activity in PTZ model and this effect may be mediated, at least partly, through interacting with nitric oxide and GABAA-BZD receptor complex.

View Article: PubMed Central - PubMed

Affiliation: School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran.

ABSTRACT

Objective: Regarding the anticonvulsant effects of Satureja hortensis (S . hortensis) in Avicenna's book: canon of medicine; the present study was undertaken to evaluate the anti- eplileptic effects of S . hortensis aqueous and ethanolic aerial part extracts. Furthermore, the mechanisms of their anticonvulsant activities were also evaluated.

Materials and methods: Seizure was induced by Pentylentetrazol (PTZ) and MES (maximal electroshock) models. Mice were randomly divided into 8 groups; negative control (normal saline, 10ml/Kg), positive control (diazepam, 2 mg/kg), S. hortensis aqueous and ethanolic extracts (200, 400 and 600 mg/kg). In PTZ test, latency to the first minimal clonic seizure (MCS), latency to the first generalized tonic-clonic seizures (GTCS), the total duration of seizures and protection against mortality were evaluated. In MES test, the stretching length of extremities and protection against mortality were recorded.

Results: Aqueous and ethanolic extracts (400 and 600 mg/kg) significantly increased MCS and GTCS latencies in PTZ model. Three doses of the extracts decreased the total duration of seizure. These extracts did not show any protective effects on seizure induced by MES model. In PTZ model, flumazenil, an antagonist of benzodiazepine (BZD) site in the GABAA-BZD receptor complex and 7- nitroindazole (7- NI), a selective nNOS (neuronal nitric oxide synthase) inhibitor, reduced the prolongation of seizure latency.

Conclusion: S. hortensis showed anticonvulsant activity in PTZ model and this effect may be mediated, at least partly, through interacting with nitric oxide and GABAA-BZD receptor complex.

No MeSH data available.


Related in: MedlinePlus