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Antioxidant activity and phenolic profiles of the wild currant Ribes magellanicum from Chilean and Argentinean Patagonia.

Jiménez-Aspee F, Thomas-Valdés S, Schulz A, Ladio A, Theoduloz C, Schmeda-Hirschmann G - Food Sci Nutr (2015)

Bottom Line: Best cytoprotection against oxidative stress induced by H2O2 in AGS cells was found in one Argentinean sample at 500 μg mL(-1) (65.7%).HCA content was about three times higher in Argentinean samples.The phenolic profiles of Chilean and Argentinean Ribes magellanicum show remarkable differences in chemical composition with higher HCA and flavonoid content in Argentinean samples.

View Article: PubMed Central - PubMed

Affiliation: Laboratorio de Química de Productos Naturales Instituto de Química de Recursos Naturales Universidad de Talca Talca Chile.

ABSTRACT
The Patagonian currant Ribes magellanicum is highly valued due to its pleasant flavor and sweet taste. The aim of this study was to characterize its constituents and to assess their antioxidant and cytoprotective properties. For the fruit phenolic-enriched extract (PEE), total phenolics (TP), total flavonoids (TF), and antioxidant activity (DPPH, Ferric reducing antioxidant power (FRAP), and Trolox equivalent antioxidant activity (TEAC)) were determined. Argentinean samples presented better activity in the DPPH and FRAP assays. Best cytoprotection against oxidative stress induced by H2O2 in AGS cells was found in one Argentinean sample at 500 μg mL(-1) (65.7%). HPLC MS/MS analysis allowed the tentative identification of 59 constituents, including eight anthocyanins, 11 conjugates of caffeic-, ferulic-, and coumaric acid, and 38 flavonoids, most of them quercetin and kaempferol derivatives. Argentinean samples showed a more complex pattern of anthocyanins, hydroxycinnamic acids (HCA), and flavonoids. Cyanidin rhamnoside hexoside and cyanidin hexoside were the main anthocyanins, accounting for 35 and 55% for the Argentinean and 60 and 27% for the ripe Chilean fruits. HCA content was about three times higher in Argentinean samples. The phenolic profiles of Chilean and Argentinean Ribes magellanicum show remarkable differences in chemical composition with higher HCA and flavonoid content in Argentinean samples.

No MeSH data available.


Related in: MedlinePlus

Scatter plot of the first and second discriminating factors showing differences between Argentinean (Laguna Verde, Villa La Angostura, and Arroyo Casa de Piedra) and Chilean (Reserva Nacional Malalcahuello and Parque Nacional Conguillio) Ribes magellanicum samples.
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fsn3323-fig-0006: Scatter plot of the first and second discriminating factors showing differences between Argentinean (Laguna Verde, Villa La Angostura, and Arroyo Casa de Piedra) and Chilean (Reserva Nacional Malalcahuello and Parque Nacional Conguillio) Ribes magellanicum samples.

Mentions: Among other chemometric tools, principal component analysis (PCA) is commonly used for discrimination, classification, modeling, and correlation (Di Paola‐Naranjo et al. 2011). As presented in Figure 6, the samples were classified into three clearly separated groups. The first discriminating factor accounts for 67.33% of the variability and the second discriminating factor for 25.83% of the variability. The TF (−0.9966), FRAP (−0.9994), and compounds 33 (−0.9600), 20 (−0.9983), 22 (−0.9868), 37 (−0.9888), 41–43 (−0.9950), 54 (−0.9874), and 56 (−0.9788) showed negative correlation and influenced more significantly the first discriminating factor. On the other hand, compounds 11 (0.9975), 4 (0.9865), and 19 (−0.9899) exerted more effect on the second discriminating factor. This distinction between Chilean and Argentinean Ribes magellanicum fruits (Fig. 6) can be considered a first approach; however, more samples are needed in order to obtain a more complete picture.


Antioxidant activity and phenolic profiles of the wild currant Ribes magellanicum from Chilean and Argentinean Patagonia.

Jiménez-Aspee F, Thomas-Valdés S, Schulz A, Ladio A, Theoduloz C, Schmeda-Hirschmann G - Food Sci Nutr (2015)

Scatter plot of the first and second discriminating factors showing differences between Argentinean (Laguna Verde, Villa La Angostura, and Arroyo Casa de Piedra) and Chilean (Reserva Nacional Malalcahuello and Parque Nacional Conguillio) Ribes magellanicum samples.
© Copyright Policy - creativeCommonsBy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4930503&req=5

fsn3323-fig-0006: Scatter plot of the first and second discriminating factors showing differences between Argentinean (Laguna Verde, Villa La Angostura, and Arroyo Casa de Piedra) and Chilean (Reserva Nacional Malalcahuello and Parque Nacional Conguillio) Ribes magellanicum samples.
Mentions: Among other chemometric tools, principal component analysis (PCA) is commonly used for discrimination, classification, modeling, and correlation (Di Paola‐Naranjo et al. 2011). As presented in Figure 6, the samples were classified into three clearly separated groups. The first discriminating factor accounts for 67.33% of the variability and the second discriminating factor for 25.83% of the variability. The TF (−0.9966), FRAP (−0.9994), and compounds 33 (−0.9600), 20 (−0.9983), 22 (−0.9868), 37 (−0.9888), 41–43 (−0.9950), 54 (−0.9874), and 56 (−0.9788) showed negative correlation and influenced more significantly the first discriminating factor. On the other hand, compounds 11 (0.9975), 4 (0.9865), and 19 (−0.9899) exerted more effect on the second discriminating factor. This distinction between Chilean and Argentinean Ribes magellanicum fruits (Fig. 6) can be considered a first approach; however, more samples are needed in order to obtain a more complete picture.

Bottom Line: Best cytoprotection against oxidative stress induced by H2O2 in AGS cells was found in one Argentinean sample at 500 μg mL(-1) (65.7%).HCA content was about three times higher in Argentinean samples.The phenolic profiles of Chilean and Argentinean Ribes magellanicum show remarkable differences in chemical composition with higher HCA and flavonoid content in Argentinean samples.

View Article: PubMed Central - PubMed

Affiliation: Laboratorio de Química de Productos Naturales Instituto de Química de Recursos Naturales Universidad de Talca Talca Chile.

ABSTRACT
The Patagonian currant Ribes magellanicum is highly valued due to its pleasant flavor and sweet taste. The aim of this study was to characterize its constituents and to assess their antioxidant and cytoprotective properties. For the fruit phenolic-enriched extract (PEE), total phenolics (TP), total flavonoids (TF), and antioxidant activity (DPPH, Ferric reducing antioxidant power (FRAP), and Trolox equivalent antioxidant activity (TEAC)) were determined. Argentinean samples presented better activity in the DPPH and FRAP assays. Best cytoprotection against oxidative stress induced by H2O2 in AGS cells was found in one Argentinean sample at 500 μg mL(-1) (65.7%). HPLC MS/MS analysis allowed the tentative identification of 59 constituents, including eight anthocyanins, 11 conjugates of caffeic-, ferulic-, and coumaric acid, and 38 flavonoids, most of them quercetin and kaempferol derivatives. Argentinean samples showed a more complex pattern of anthocyanins, hydroxycinnamic acids (HCA), and flavonoids. Cyanidin rhamnoside hexoside and cyanidin hexoside were the main anthocyanins, accounting for 35 and 55% for the Argentinean and 60 and 27% for the ripe Chilean fruits. HCA content was about three times higher in Argentinean samples. The phenolic profiles of Chilean and Argentinean Ribes magellanicum show remarkable differences in chemical composition with higher HCA and flavonoid content in Argentinean samples.

No MeSH data available.


Related in: MedlinePlus