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Antioxidant activity and phenolic profiles of the wild currant Ribes magellanicum from Chilean and Argentinean Patagonia.

Jiménez-Aspee F, Thomas-Valdés S, Schulz A, Ladio A, Theoduloz C, Schmeda-Hirschmann G - Food Sci Nutr (2015)

Bottom Line: Best cytoprotection against oxidative stress induced by H2O2 in AGS cells was found in one Argentinean sample at 500 μg mL(-1) (65.7%).HCA content was about three times higher in Argentinean samples.The phenolic profiles of Chilean and Argentinean Ribes magellanicum show remarkable differences in chemical composition with higher HCA and flavonoid content in Argentinean samples.

View Article: PubMed Central - PubMed

Affiliation: Laboratorio de Química de Productos Naturales Instituto de Química de Recursos Naturales Universidad de Talca Talca Chile.

ABSTRACT
The Patagonian currant Ribes magellanicum is highly valued due to its pleasant flavor and sweet taste. The aim of this study was to characterize its constituents and to assess their antioxidant and cytoprotective properties. For the fruit phenolic-enriched extract (PEE), total phenolics (TP), total flavonoids (TF), and antioxidant activity (DPPH, Ferric reducing antioxidant power (FRAP), and Trolox equivalent antioxidant activity (TEAC)) were determined. Argentinean samples presented better activity in the DPPH and FRAP assays. Best cytoprotection against oxidative stress induced by H2O2 in AGS cells was found in one Argentinean sample at 500 μg mL(-1) (65.7%). HPLC MS/MS analysis allowed the tentative identification of 59 constituents, including eight anthocyanins, 11 conjugates of caffeic-, ferulic-, and coumaric acid, and 38 flavonoids, most of them quercetin and kaempferol derivatives. Argentinean samples showed a more complex pattern of anthocyanins, hydroxycinnamic acids (HCA), and flavonoids. Cyanidin rhamnoside hexoside and cyanidin hexoside were the main anthocyanins, accounting for 35 and 55% for the Argentinean and 60 and 27% for the ripe Chilean fruits. HCA content was about three times higher in Argentinean samples. The phenolic profiles of Chilean and Argentinean Ribes magellanicum show remarkable differences in chemical composition with higher HCA and flavonoid content in Argentinean samples.

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Related in: MedlinePlus

Cytoprotective effect of Ribes magellanicum PEEs against H2O2‐induced oxidative stress on AGS cells. (A) Argentinean samples, (B) Chilean samples. The cell viability was determined by MTT reduction assay. Results are expressed as means ± SD (n = 5). *P < 0.05 compared to H2O2 controls.
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fsn3323-fig-0003: Cytoprotective effect of Ribes magellanicum PEEs against H2O2‐induced oxidative stress on AGS cells. (A) Argentinean samples, (B) Chilean samples. The cell viability was determined by MTT reduction assay. Results are expressed as means ± SD (n = 5). *P < 0.05 compared to H2O2 controls.

Mentions: Under our assay conditions, PEEs were devoid of toxicity toward AGS cells, with IC50 values >1000 mg L−1. In MRC‐5 cells a 10,000 mg L−1Ribes nigrum extract concentration induced 20% decrease in cell viability and was selected for further experiments (Jia et al. 2014). We selected 500 mg L−1 of PEE for the following studies as the highest concentration. Ribes magellanicum PEEs showed a significant cytoprotective dose‐dependent effect against oxidative damage induced by H2O2 in AGS cells. Hydrogen peroxide (442 mg L−1) caused a significant decrease in cell viability (57.3%) compared to untreated controls. Exogenous H2O2 can enter cells inducing cytotoxicity due to its high membrane permeability, and through Fenton reaction can form highly reactive ·OH species causing oxidative injury in cells (Halliwell et al. 1992; Halliwell 1994). The positive control quercetin (100 mg L−1) did not protect cells from oxidative damage. From the Argentinean samples, Arroyo Casa de Piedra showed significant protective effect at 125 mg L−1 (20.0%), 250 mg L−1 (42.9%), and 500 mg L−1 (65.7%), compared with the H2O2 control (Fig. 3A). The sample from Villa La Angostura presented significant activity at 250 mg L−1 (22.9%) and 500 mg L−1 (42.9%) (Fig. 3A). From the Chilean samples, only the PEE from Conguillio presented significant activity at 250 mg L−1 (15.2%) and 500 mg L−1 (27.3%) (Fig. 3B).


Antioxidant activity and phenolic profiles of the wild currant Ribes magellanicum from Chilean and Argentinean Patagonia.

Jiménez-Aspee F, Thomas-Valdés S, Schulz A, Ladio A, Theoduloz C, Schmeda-Hirschmann G - Food Sci Nutr (2015)

Cytoprotective effect of Ribes magellanicum PEEs against H2O2‐induced oxidative stress on AGS cells. (A) Argentinean samples, (B) Chilean samples. The cell viability was determined by MTT reduction assay. Results are expressed as means ± SD (n = 5). *P < 0.05 compared to H2O2 controls.
© Copyright Policy - creativeCommonsBy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4930503&req=5

fsn3323-fig-0003: Cytoprotective effect of Ribes magellanicum PEEs against H2O2‐induced oxidative stress on AGS cells. (A) Argentinean samples, (B) Chilean samples. The cell viability was determined by MTT reduction assay. Results are expressed as means ± SD (n = 5). *P < 0.05 compared to H2O2 controls.
Mentions: Under our assay conditions, PEEs were devoid of toxicity toward AGS cells, with IC50 values >1000 mg L−1. In MRC‐5 cells a 10,000 mg L−1Ribes nigrum extract concentration induced 20% decrease in cell viability and was selected for further experiments (Jia et al. 2014). We selected 500 mg L−1 of PEE for the following studies as the highest concentration. Ribes magellanicum PEEs showed a significant cytoprotective dose‐dependent effect against oxidative damage induced by H2O2 in AGS cells. Hydrogen peroxide (442 mg L−1) caused a significant decrease in cell viability (57.3%) compared to untreated controls. Exogenous H2O2 can enter cells inducing cytotoxicity due to its high membrane permeability, and through Fenton reaction can form highly reactive ·OH species causing oxidative injury in cells (Halliwell et al. 1992; Halliwell 1994). The positive control quercetin (100 mg L−1) did not protect cells from oxidative damage. From the Argentinean samples, Arroyo Casa de Piedra showed significant protective effect at 125 mg L−1 (20.0%), 250 mg L−1 (42.9%), and 500 mg L−1 (65.7%), compared with the H2O2 control (Fig. 3A). The sample from Villa La Angostura presented significant activity at 250 mg L−1 (22.9%) and 500 mg L−1 (42.9%) (Fig. 3A). From the Chilean samples, only the PEE from Conguillio presented significant activity at 250 mg L−1 (15.2%) and 500 mg L−1 (27.3%) (Fig. 3B).

Bottom Line: Best cytoprotection against oxidative stress induced by H2O2 in AGS cells was found in one Argentinean sample at 500 μg mL(-1) (65.7%).HCA content was about three times higher in Argentinean samples.The phenolic profiles of Chilean and Argentinean Ribes magellanicum show remarkable differences in chemical composition with higher HCA and flavonoid content in Argentinean samples.

View Article: PubMed Central - PubMed

Affiliation: Laboratorio de Química de Productos Naturales Instituto de Química de Recursos Naturales Universidad de Talca Talca Chile.

ABSTRACT
The Patagonian currant Ribes magellanicum is highly valued due to its pleasant flavor and sweet taste. The aim of this study was to characterize its constituents and to assess their antioxidant and cytoprotective properties. For the fruit phenolic-enriched extract (PEE), total phenolics (TP), total flavonoids (TF), and antioxidant activity (DPPH, Ferric reducing antioxidant power (FRAP), and Trolox equivalent antioxidant activity (TEAC)) were determined. Argentinean samples presented better activity in the DPPH and FRAP assays. Best cytoprotection against oxidative stress induced by H2O2 in AGS cells was found in one Argentinean sample at 500 μg mL(-1) (65.7%). HPLC MS/MS analysis allowed the tentative identification of 59 constituents, including eight anthocyanins, 11 conjugates of caffeic-, ferulic-, and coumaric acid, and 38 flavonoids, most of them quercetin and kaempferol derivatives. Argentinean samples showed a more complex pattern of anthocyanins, hydroxycinnamic acids (HCA), and flavonoids. Cyanidin rhamnoside hexoside and cyanidin hexoside were the main anthocyanins, accounting for 35 and 55% for the Argentinean and 60 and 27% for the ripe Chilean fruits. HCA content was about three times higher in Argentinean samples. The phenolic profiles of Chilean and Argentinean Ribes magellanicum show remarkable differences in chemical composition with higher HCA and flavonoid content in Argentinean samples.

No MeSH data available.


Related in: MedlinePlus