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Induction and differentiation of adipose-derived stem cells from human buccal fat pads into salivary gland cells.

Kawakami M, Ishikawa H, Tanaka A, Mataga I - Hum. Cell (2016)

Bottom Line: Co-cultured cells expressed salivary-glandrelated markers and generated new tissues following transplantation in vivo.Moreover, cell reconstituted glandular structures in the 3D culture system.In conclusion, coculture of hSG-fibros with hBFP-ASCs led to successful differentiation into salivary gland cells that could be transplanted to generate new tissues.

View Article: PubMed Central - PubMed

Affiliation: Department of Oral and Maxillofacial Surgery, School of Life Dentistry at Niigata, The Nippon Dental University, 1-8 Hamaura-cho, Chuo-ku, Niigata, 951-8580, Japan. miu-k@tky.ndu.ac.jp.

ABSTRACT
Atrophy or hypofunction of the salivary gland because of aging or disease leads to hyposalivation that affects patient quality of life by causing dry mouth, deterioration of mastication/deglutition, and poor oral hygiene status. Current therapy for atrophy or hypofunction of the salivary gland in clinical practice focuses on symptom relief using drugs and artificial saliva; therefore, there is still a need to develop new therapies. To investigate potential novel therapeutic targets, we induced the differentiation of salivary gland cells by co-culturing human adipose-derived stem cells isolated from buccal fat pads (hBFP-ASCs) with human salivary-gland-derived fibroblasts (hSG-fibros). We examined their potential for transplantation and tissue neogenesis. Following the culture of hBFP-ASCs and hSG-fibros, differentiated cells were transplanted into the submandibular glands of SCID mice, and their degree of differentiation in tissues was determined. We also examined their potential for functional tissue reconstitution using a three-dimensional (3D) culture system. Co-cultured cells expressed salivary-glandrelated markers and generated new tissues following transplantation in vivo. Moreover, cell reconstituted glandular structures in the 3D culture system. In conclusion, coculture of hSG-fibros with hBFP-ASCs led to successful differentiation into salivary gland cells that could be transplanted to generate new tissues.

No MeSH data available.


Related in: MedlinePlus

Identification of hSG-fibros. a Phase-contrast micrographs of typical spindle shapes. Scale bar 100 µm. b–d Immunostained images. DAPI (blue), b vimentin, and c amylase (red). d RT-PCR confirmed the cells were human-derived fibroblasts
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Fig2: Identification of hSG-fibros. a Phase-contrast micrographs of typical spindle shapes. Scale bar 100 µm. b–d Immunostained images. DAPI (blue), b vimentin, and c amylase (red). d RT-PCR confirmed the cells were human-derived fibroblasts

Mentions: Cultured fibroblasts with typical spindle-shaped morphology by phase-contrast microscopy (Fig. 2a) were analyzed by immunostaining (Fig. 2b) and RT-PCR (Fig. 2d), which indicated they were hSG-fibros. Amylase analysis by RT-PCR and immunostaining revealed no expression, indicating cells isolated from salivary glands did not include acinar cell components (Fig. 2c,d).Fig. 2


Induction and differentiation of adipose-derived stem cells from human buccal fat pads into salivary gland cells.

Kawakami M, Ishikawa H, Tanaka A, Mataga I - Hum. Cell (2016)

Identification of hSG-fibros. a Phase-contrast micrographs of typical spindle shapes. Scale bar 100 µm. b–d Immunostained images. DAPI (blue), b vimentin, and c amylase (red). d RT-PCR confirmed the cells were human-derived fibroblasts
© Copyright Policy - OpenAccess
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4930478&req=5

Fig2: Identification of hSG-fibros. a Phase-contrast micrographs of typical spindle shapes. Scale bar 100 µm. b–d Immunostained images. DAPI (blue), b vimentin, and c amylase (red). d RT-PCR confirmed the cells were human-derived fibroblasts
Mentions: Cultured fibroblasts with typical spindle-shaped morphology by phase-contrast microscopy (Fig. 2a) were analyzed by immunostaining (Fig. 2b) and RT-PCR (Fig. 2d), which indicated they were hSG-fibros. Amylase analysis by RT-PCR and immunostaining revealed no expression, indicating cells isolated from salivary glands did not include acinar cell components (Fig. 2c,d).Fig. 2

Bottom Line: Co-cultured cells expressed salivary-glandrelated markers and generated new tissues following transplantation in vivo.Moreover, cell reconstituted glandular structures in the 3D culture system.In conclusion, coculture of hSG-fibros with hBFP-ASCs led to successful differentiation into salivary gland cells that could be transplanted to generate new tissues.

View Article: PubMed Central - PubMed

Affiliation: Department of Oral and Maxillofacial Surgery, School of Life Dentistry at Niigata, The Nippon Dental University, 1-8 Hamaura-cho, Chuo-ku, Niigata, 951-8580, Japan. miu-k@tky.ndu.ac.jp.

ABSTRACT
Atrophy or hypofunction of the salivary gland because of aging or disease leads to hyposalivation that affects patient quality of life by causing dry mouth, deterioration of mastication/deglutition, and poor oral hygiene status. Current therapy for atrophy or hypofunction of the salivary gland in clinical practice focuses on symptom relief using drugs and artificial saliva; therefore, there is still a need to develop new therapies. To investigate potential novel therapeutic targets, we induced the differentiation of salivary gland cells by co-culturing human adipose-derived stem cells isolated from buccal fat pads (hBFP-ASCs) with human salivary-gland-derived fibroblasts (hSG-fibros). We examined their potential for transplantation and tissue neogenesis. Following the culture of hBFP-ASCs and hSG-fibros, differentiated cells were transplanted into the submandibular glands of SCID mice, and their degree of differentiation in tissues was determined. We also examined their potential for functional tissue reconstitution using a three-dimensional (3D) culture system. Co-cultured cells expressed salivary-glandrelated markers and generated new tissues following transplantation in vivo. Moreover, cell reconstituted glandular structures in the 3D culture system. In conclusion, coculture of hSG-fibros with hBFP-ASCs led to successful differentiation into salivary gland cells that could be transplanted to generate new tissues.

No MeSH data available.


Related in: MedlinePlus