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The relationship between the violet pigment PP-V production and intracellular ammonium level in Penicillium purpurogenum.

Kojima R, Arai T, Matsufuji H, Kasumi T, Watanabe T, Ogihara J - AMB Express (2016)

Bottom Line: This pigment contains an amino group in a pyran ring as its core structure.We focused on this pigment and examined the relationship between intracellular ammonium concentration and pigment production using glutamine as a nitrogen source.From comparisons of the amount of PP-V that was secreted with quantity of surplus intracellular ammonium, it is suggested that P. purpurogenum maintains ammonium homeostasis by excreting waste ammonium as PP-V.

View Article: PubMed Central - PubMed

Affiliation: Graduate School of Bioresource Sciences, Nihon University, 1866 Kameino, Fujisawa, Kanagawa, 252-0880, Japan.

ABSTRACT
Penicillium purpurogenum is the fungus that produces an azaphilone pigment. However, details about the pigment biosynthesis pathway are unknown. The violet pigment PP-V is the one of the main pigments biosynthesized by this fungus. This pigment contains an amino group in a pyran ring as its core structure. We focused on this pigment and examined the relationship between intracellular ammonium concentration and pigment production using glutamine as a nitrogen source. The intracellular ammonium level decreased about 1.5-fold in conditions favoring PP-V production. Moreover, P. purpurogenum was transferred to medium in which it commonly produces the related pigment PP-O after cultivating it in the presence or absence of glutamine to investigate whether this fungus biosynthesizes PP-V using surplus ammonium in cells. Only mycelia cultured in medium containing 10 mM glutamine produced the violet pigment, and simultaneously intracellular ammonium levels decreased under this condition. From comparisons of the amount of PP-V that was secreted with quantity of surplus intracellular ammonium, it is suggested that P. purpurogenum maintains ammonium homeostasis by excreting waste ammonium as PP-V.

No MeSH data available.


Related in: MedlinePlus

Effect of glutamine on PP-V production. Penicillium purpurogenum IAM15392 was grown in 500-mL Erlenmeyer flasks containing 100 mL basal medium or glutamine medium (f.c. 1 or 10 mM l-glutamine) for 72–96 h at 30 °C with shaking at 200 rpm. For TLC, the culture broth, and PP-O and glutamine reaction solution obtained in vitro were centrifuged (1600×g, 4 °C, 15 min). The pigment in the supernatant was extracted with EtOAc. Pigments were detected by TLC using a silica gel 60 plate with developing solvent mixture n-BuOH: AcOH: H2O (12:3:5)
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Fig1: Effect of glutamine on PP-V production. Penicillium purpurogenum IAM15392 was grown in 500-mL Erlenmeyer flasks containing 100 mL basal medium or glutamine medium (f.c. 1 or 10 mM l-glutamine) for 72–96 h at 30 °C with shaking at 200 rpm. For TLC, the culture broth, and PP-O and glutamine reaction solution obtained in vitro were centrifuged (1600×g, 4 °C, 15 min). The pigment in the supernatant was extracted with EtOAc. Pigments were detected by TLC using a silica gel 60 plate with developing solvent mixture n-BuOH: AcOH: H2O (12:3:5)

Mentions: The effect of glutamine on PP-V production is shown in Fig. 1. PP-V was not detected in the in vitro reaction mixture of culture filtrates containing PP-O and glutamine. Moreover, only PP-O was produced in basal medium and 1 mM glutamine medium after 72 and 96 h. In 10 mM glutamine medium, PP-V was produced after 96 h.Fig. 1


The relationship between the violet pigment PP-V production and intracellular ammonium level in Penicillium purpurogenum.

Kojima R, Arai T, Matsufuji H, Kasumi T, Watanabe T, Ogihara J - AMB Express (2016)

Effect of glutamine on PP-V production. Penicillium purpurogenum IAM15392 was grown in 500-mL Erlenmeyer flasks containing 100 mL basal medium or glutamine medium (f.c. 1 or 10 mM l-glutamine) for 72–96 h at 30 °C with shaking at 200 rpm. For TLC, the culture broth, and PP-O and glutamine reaction solution obtained in vitro were centrifuged (1600×g, 4 °C, 15 min). The pigment in the supernatant was extracted with EtOAc. Pigments were detected by TLC using a silica gel 60 plate with developing solvent mixture n-BuOH: AcOH: H2O (12:3:5)
© Copyright Policy - OpenAccess
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4930434&req=5

Fig1: Effect of glutamine on PP-V production. Penicillium purpurogenum IAM15392 was grown in 500-mL Erlenmeyer flasks containing 100 mL basal medium or glutamine medium (f.c. 1 or 10 mM l-glutamine) for 72–96 h at 30 °C with shaking at 200 rpm. For TLC, the culture broth, and PP-O and glutamine reaction solution obtained in vitro were centrifuged (1600×g, 4 °C, 15 min). The pigment in the supernatant was extracted with EtOAc. Pigments were detected by TLC using a silica gel 60 plate with developing solvent mixture n-BuOH: AcOH: H2O (12:3:5)
Mentions: The effect of glutamine on PP-V production is shown in Fig. 1. PP-V was not detected in the in vitro reaction mixture of culture filtrates containing PP-O and glutamine. Moreover, only PP-O was produced in basal medium and 1 mM glutamine medium after 72 and 96 h. In 10 mM glutamine medium, PP-V was produced after 96 h.Fig. 1

Bottom Line: This pigment contains an amino group in a pyran ring as its core structure.We focused on this pigment and examined the relationship between intracellular ammonium concentration and pigment production using glutamine as a nitrogen source.From comparisons of the amount of PP-V that was secreted with quantity of surplus intracellular ammonium, it is suggested that P. purpurogenum maintains ammonium homeostasis by excreting waste ammonium as PP-V.

View Article: PubMed Central - PubMed

Affiliation: Graduate School of Bioresource Sciences, Nihon University, 1866 Kameino, Fujisawa, Kanagawa, 252-0880, Japan.

ABSTRACT
Penicillium purpurogenum is the fungus that produces an azaphilone pigment. However, details about the pigment biosynthesis pathway are unknown. The violet pigment PP-V is the one of the main pigments biosynthesized by this fungus. This pigment contains an amino group in a pyran ring as its core structure. We focused on this pigment and examined the relationship between intracellular ammonium concentration and pigment production using glutamine as a nitrogen source. The intracellular ammonium level decreased about 1.5-fold in conditions favoring PP-V production. Moreover, P. purpurogenum was transferred to medium in which it commonly produces the related pigment PP-O after cultivating it in the presence or absence of glutamine to investigate whether this fungus biosynthesizes PP-V using surplus ammonium in cells. Only mycelia cultured in medium containing 10 mM glutamine produced the violet pigment, and simultaneously intracellular ammonium levels decreased under this condition. From comparisons of the amount of PP-V that was secreted with quantity of surplus intracellular ammonium, it is suggested that P. purpurogenum maintains ammonium homeostasis by excreting waste ammonium as PP-V.

No MeSH data available.


Related in: MedlinePlus