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Neurotensin Changes Propulsive Activity into a Segmental Motor Pattern in the Rat Colon.

Li H, Chen JH, Yang Z, Huang M, Yu Y, Tan S, Luo H, Huizinga JD - J Neurogastroenterol Motil (2016)

Bottom Line: High concentrations given without prior additions of low concentrations did not evoke the segmental motor pattern.These actions occurred when neurotensin was given in the bath solution or intraluminally.The segmental motor pattern evoked by neurotensin was inhibited by the neural conduction blocker lidocaine.

View Article: PubMed Central - PubMed

Affiliation: Department of Gastroenterology and Hepatology, Renmin Hospital of Wuhan University, Key Laboratory of Hubei Province for Digestive System Diseases, Wuhan, Hubei Province, China.

ABSTRACT

Background/aims: Neurotensin is a gut-brain peptide with both inhibitory and excitatory actions on the colonic musculature; our objective was to understand the implications of this for motor patterns occurring in the intact colon of the rat.

Methods: The effects of neurotensin with concentrations ranging from 0.1-100 nM were studied in the intact rat colon in vitro, by investigating spatio-temporal maps created from video recordings of colonic motility before and after neurotensin.

Results: Low concentration of neurotensin (0.1-1 nM) inhibited propagating long distance contractions and rhythmic propagating motor complexes; in its place a slow propagating rhythmic segmental motor pattern developed. The neurotensin receptor 1 antagonist SR-48692 prevented the development of the segmental motor pattern. Higher concentrations of neurotensin (10 nM and 100 nM) were capable of restoring long distance contraction activity and inhibiting the segmental activity. The slow propagating segmental contraction showed a rhythmic contraction-- relaxation cycle at the slow wave frequency originating from the interstitial cells of Cajal associated with the myenteric plexus pacemaker. High concentrations given without prior additions of low concentrations did not evoke the segmental motor pattern. These actions occurred when neurotensin was given in the bath solution or intraluminally. The segmental motor pattern evoked by neurotensin was inhibited by the neural conduction blocker lidocaine.

Conclusions: Neurotensin (0.1-1 nM) inhibits the dominant propulsive motor patterns of the colon and a distinct motor pattern of rhythmic slow propagating segmental contractions develops. This motor pattern has the hallmarks of haustral boundary contractions.

No MeSH data available.


Related in: MedlinePlus

Intraluminal neurotensin changes long distance contraction (LDC) activity into slow propagating segmental contractions.Experiment 1: (A) Baseline activity shows regular rhythmic LDC activity at 0.6 cpm. (B) Neurotensin (0.1 nM) was added into the lumen 10 minutes before recording. LDCs changed into slow propagating segmental contractions. Strong proximal contractions remained at 0.6 cpm.Experiment 2: (C) Neurotensin (0.1 nM) was added at arrow. LDCs change into slow propagating segmental contractions. Thereafter the concentration was increased to 0.5 nM neurotensin (D).
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f4-jnm-22-517: Intraluminal neurotensin changes long distance contraction (LDC) activity into slow propagating segmental contractions.Experiment 1: (A) Baseline activity shows regular rhythmic LDC activity at 0.6 cpm. (B) Neurotensin (0.1 nM) was added into the lumen 10 minutes before recording. LDCs changed into slow propagating segmental contractions. Strong proximal contractions remained at 0.6 cpm.Experiment 2: (C) Neurotensin (0.1 nM) was added at arrow. LDCs change into slow propagating segmental contractions. Thereafter the concentration was increased to 0.5 nM neurotensin (D).

Mentions: When neurotensin was given in the same concentration range as described above into the lumen of the colon, the results related to LDCs and slow propagating segmental contractions were similar (Fig. 4; Tables 4 and 5). RPMC activity was inhibited at the intraluminal neurotensin concentration of 0.1 nM; no RPMC activity was observed in the presence of 0.5 nM luminal neurotensin or higher.


Neurotensin Changes Propulsive Activity into a Segmental Motor Pattern in the Rat Colon.

Li H, Chen JH, Yang Z, Huang M, Yu Y, Tan S, Luo H, Huizinga JD - J Neurogastroenterol Motil (2016)

Intraluminal neurotensin changes long distance contraction (LDC) activity into slow propagating segmental contractions.Experiment 1: (A) Baseline activity shows regular rhythmic LDC activity at 0.6 cpm. (B) Neurotensin (0.1 nM) was added into the lumen 10 minutes before recording. LDCs changed into slow propagating segmental contractions. Strong proximal contractions remained at 0.6 cpm.Experiment 2: (C) Neurotensin (0.1 nM) was added at arrow. LDCs change into slow propagating segmental contractions. Thereafter the concentration was increased to 0.5 nM neurotensin (D).
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4930308&req=5

f4-jnm-22-517: Intraluminal neurotensin changes long distance contraction (LDC) activity into slow propagating segmental contractions.Experiment 1: (A) Baseline activity shows regular rhythmic LDC activity at 0.6 cpm. (B) Neurotensin (0.1 nM) was added into the lumen 10 minutes before recording. LDCs changed into slow propagating segmental contractions. Strong proximal contractions remained at 0.6 cpm.Experiment 2: (C) Neurotensin (0.1 nM) was added at arrow. LDCs change into slow propagating segmental contractions. Thereafter the concentration was increased to 0.5 nM neurotensin (D).
Mentions: When neurotensin was given in the same concentration range as described above into the lumen of the colon, the results related to LDCs and slow propagating segmental contractions were similar (Fig. 4; Tables 4 and 5). RPMC activity was inhibited at the intraluminal neurotensin concentration of 0.1 nM; no RPMC activity was observed in the presence of 0.5 nM luminal neurotensin or higher.

Bottom Line: High concentrations given without prior additions of low concentrations did not evoke the segmental motor pattern.These actions occurred when neurotensin was given in the bath solution or intraluminally.The segmental motor pattern evoked by neurotensin was inhibited by the neural conduction blocker lidocaine.

View Article: PubMed Central - PubMed

Affiliation: Department of Gastroenterology and Hepatology, Renmin Hospital of Wuhan University, Key Laboratory of Hubei Province for Digestive System Diseases, Wuhan, Hubei Province, China.

ABSTRACT

Background/aims: Neurotensin is a gut-brain peptide with both inhibitory and excitatory actions on the colonic musculature; our objective was to understand the implications of this for motor patterns occurring in the intact colon of the rat.

Methods: The effects of neurotensin with concentrations ranging from 0.1-100 nM were studied in the intact rat colon in vitro, by investigating spatio-temporal maps created from video recordings of colonic motility before and after neurotensin.

Results: Low concentration of neurotensin (0.1-1 nM) inhibited propagating long distance contractions and rhythmic propagating motor complexes; in its place a slow propagating rhythmic segmental motor pattern developed. The neurotensin receptor 1 antagonist SR-48692 prevented the development of the segmental motor pattern. Higher concentrations of neurotensin (10 nM and 100 nM) were capable of restoring long distance contraction activity and inhibiting the segmental activity. The slow propagating segmental contraction showed a rhythmic contraction-- relaxation cycle at the slow wave frequency originating from the interstitial cells of Cajal associated with the myenteric plexus pacemaker. High concentrations given without prior additions of low concentrations did not evoke the segmental motor pattern. These actions occurred when neurotensin was given in the bath solution or intraluminally. The segmental motor pattern evoked by neurotensin was inhibited by the neural conduction blocker lidocaine.

Conclusions: Neurotensin (0.1-1 nM) inhibits the dominant propulsive motor patterns of the colon and a distinct motor pattern of rhythmic slow propagating segmental contractions develops. This motor pattern has the hallmarks of haustral boundary contractions.

No MeSH data available.


Related in: MedlinePlus