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Aloe-Emodin Induces Chondrogenic Differentiation of ATDC5 Cells via MAP Kinases and BMP-2 Signaling Pathways.

Yang M, Li L, Heo SM, Soh Y - Biomol Ther (Seoul) (2016)

Bottom Line: Furthermore, examination of the MAPK signaling pathway showed that aloe-emodin increased the activation of extracellular signal-regulated kinase (ERK), but had no effect on p38 and c-jun N-terminal kinase (JNK).Thus, these results showed that aloe-emodin exhibited chodromodulating effects via the BMP-2 or ERK signaling pathway.Aloe-emodin may have potential future applications for the treatment of growth disorders.

View Article: PubMed Central - PubMed

Affiliation: Department of Periodontology, School of Dentistry, Chonbuk National University, Jeonju 54896, Republic of Korea.

ABSTRACT
Endochondral bone formation is the process by which mesenchymal cells condense into chondrocytes, which are ultimately responsible for new bone formation. The processes of chondrogenic differentiation and hypertrophy are critical for bone formation and are therefore highly regulated. The present study was designed to investigate the effect of aloe-emodin on chondrogenic differentiation in clonal mouse chondrogenic ATDC5 cells. Aloe-emodin treatment stimulated the accumulation of cartilage nodules in a dose-dependent manner. ATDC5 cells were treated with aloe-emodin and stained with alcian blue. Compared with the control cells, the ATDC5 cells showed more intense alcian blue staining. This finding suggested that aloe-emodin induced the synthesis of matrix proteoglycans and increased the activity of alkaline phosphatase. Aloe-emodin also enhanced the expressions of chondrogenic marker genes such as collagen II, collagen X, BSP and RunX2 in a time-dependent manner. Furthermore, examination of the MAPK signaling pathway showed that aloe-emodin increased the activation of extracellular signal-regulated kinase (ERK), but had no effect on p38 and c-jun N-terminal kinase (JNK). Aloe-emodin also enhanced the protein expression of BMP-2 in a time-dependent manner. Thus, these results showed that aloe-emodin exhibited chodromodulating effects via the BMP-2 or ERK signaling pathway. Aloe-emodin may have potential future applications for the treatment of growth disorders.

No MeSH data available.


Related in: MedlinePlus

Effect of MAP kinase inhibitor on chondrogenesis with aloe-emodin in ATDC5 cells. ATDC5 cells were treated with aloe-emodin and seeded at a density of (5×104) cells per well for 14 days in the presence or absence of 20 μM PD 98059 (A). Stained cells were dissolved in 10% acetic acid for subsequent quantification of the absorbance at 650 nm (B). Each histogram represents the mean ± S.E.M. (n=3).
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f6-bt-24-395: Effect of MAP kinase inhibitor on chondrogenesis with aloe-emodin in ATDC5 cells. ATDC5 cells were treated with aloe-emodin and seeded at a density of (5×104) cells per well for 14 days in the presence or absence of 20 μM PD 98059 (A). Stained cells were dissolved in 10% acetic acid for subsequent quantification of the absorbance at 650 nm (B). Each histogram represents the mean ± S.E.M. (n=3).

Mentions: To demonstrate the effect of aloe-emodin on the induction of ERK during ATDC5 cell differentiation, cells were pretreated cells with 20 μM PD 98059 for 30 minutes, followed by treatment with aloe-emodin for 14 days. Alcian blue staining demonstrated that cells treated with aloe-emodin showed significant increases in the size and number of nodules while treatment with PD 98059 significantly inhibited the increase (Fig. 6). The data suggested that aloe-emodin can induce ATDC5 cells differentiation via the ERK signaling pathway.


Aloe-Emodin Induces Chondrogenic Differentiation of ATDC5 Cells via MAP Kinases and BMP-2 Signaling Pathways.

Yang M, Li L, Heo SM, Soh Y - Biomol Ther (Seoul) (2016)

Effect of MAP kinase inhibitor on chondrogenesis with aloe-emodin in ATDC5 cells. ATDC5 cells were treated with aloe-emodin and seeded at a density of (5×104) cells per well for 14 days in the presence or absence of 20 μM PD 98059 (A). Stained cells were dissolved in 10% acetic acid for subsequent quantification of the absorbance at 650 nm (B). Each histogram represents the mean ± S.E.M. (n=3).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4930283&req=5

f6-bt-24-395: Effect of MAP kinase inhibitor on chondrogenesis with aloe-emodin in ATDC5 cells. ATDC5 cells were treated with aloe-emodin and seeded at a density of (5×104) cells per well for 14 days in the presence or absence of 20 μM PD 98059 (A). Stained cells were dissolved in 10% acetic acid for subsequent quantification of the absorbance at 650 nm (B). Each histogram represents the mean ± S.E.M. (n=3).
Mentions: To demonstrate the effect of aloe-emodin on the induction of ERK during ATDC5 cell differentiation, cells were pretreated cells with 20 μM PD 98059 for 30 minutes, followed by treatment with aloe-emodin for 14 days. Alcian blue staining demonstrated that cells treated with aloe-emodin showed significant increases in the size and number of nodules while treatment with PD 98059 significantly inhibited the increase (Fig. 6). The data suggested that aloe-emodin can induce ATDC5 cells differentiation via the ERK signaling pathway.

Bottom Line: Furthermore, examination of the MAPK signaling pathway showed that aloe-emodin increased the activation of extracellular signal-regulated kinase (ERK), but had no effect on p38 and c-jun N-terminal kinase (JNK).Thus, these results showed that aloe-emodin exhibited chodromodulating effects via the BMP-2 or ERK signaling pathway.Aloe-emodin may have potential future applications for the treatment of growth disorders.

View Article: PubMed Central - PubMed

Affiliation: Department of Periodontology, School of Dentistry, Chonbuk National University, Jeonju 54896, Republic of Korea.

ABSTRACT
Endochondral bone formation is the process by which mesenchymal cells condense into chondrocytes, which are ultimately responsible for new bone formation. The processes of chondrogenic differentiation and hypertrophy are critical for bone formation and are therefore highly regulated. The present study was designed to investigate the effect of aloe-emodin on chondrogenic differentiation in clonal mouse chondrogenic ATDC5 cells. Aloe-emodin treatment stimulated the accumulation of cartilage nodules in a dose-dependent manner. ATDC5 cells were treated with aloe-emodin and stained with alcian blue. Compared with the control cells, the ATDC5 cells showed more intense alcian blue staining. This finding suggested that aloe-emodin induced the synthesis of matrix proteoglycans and increased the activity of alkaline phosphatase. Aloe-emodin also enhanced the expressions of chondrogenic marker genes such as collagen II, collagen X, BSP and RunX2 in a time-dependent manner. Furthermore, examination of the MAPK signaling pathway showed that aloe-emodin increased the activation of extracellular signal-regulated kinase (ERK), but had no effect on p38 and c-jun N-terminal kinase (JNK). Aloe-emodin also enhanced the protein expression of BMP-2 in a time-dependent manner. Thus, these results showed that aloe-emodin exhibited chodromodulating effects via the BMP-2 or ERK signaling pathway. Aloe-emodin may have potential future applications for the treatment of growth disorders.

No MeSH data available.


Related in: MedlinePlus