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Aloe-Emodin Induces Chondrogenic Differentiation of ATDC5 Cells via MAP Kinases and BMP-2 Signaling Pathways.

Yang M, Li L, Heo SM, Soh Y - Biomol Ther (Seoul) (2016)

Bottom Line: Furthermore, examination of the MAPK signaling pathway showed that aloe-emodin increased the activation of extracellular signal-regulated kinase (ERK), but had no effect on p38 and c-jun N-terminal kinase (JNK).Thus, these results showed that aloe-emodin exhibited chodromodulating effects via the BMP-2 or ERK signaling pathway.Aloe-emodin may have potential future applications for the treatment of growth disorders.

View Article: PubMed Central - PubMed

Affiliation: Department of Periodontology, School of Dentistry, Chonbuk National University, Jeonju 54896, Republic of Korea.

ABSTRACT
Endochondral bone formation is the process by which mesenchymal cells condense into chondrocytes, which are ultimately responsible for new bone formation. The processes of chondrogenic differentiation and hypertrophy are critical for bone formation and are therefore highly regulated. The present study was designed to investigate the effect of aloe-emodin on chondrogenic differentiation in clonal mouse chondrogenic ATDC5 cells. Aloe-emodin treatment stimulated the accumulation of cartilage nodules in a dose-dependent manner. ATDC5 cells were treated with aloe-emodin and stained with alcian blue. Compared with the control cells, the ATDC5 cells showed more intense alcian blue staining. This finding suggested that aloe-emodin induced the synthesis of matrix proteoglycans and increased the activity of alkaline phosphatase. Aloe-emodin also enhanced the expressions of chondrogenic marker genes such as collagen II, collagen X, BSP and RunX2 in a time-dependent manner. Furthermore, examination of the MAPK signaling pathway showed that aloe-emodin increased the activation of extracellular signal-regulated kinase (ERK), but had no effect on p38 and c-jun N-terminal kinase (JNK). Aloe-emodin also enhanced the protein expression of BMP-2 in a time-dependent manner. Thus, these results showed that aloe-emodin exhibited chodromodulating effects via the BMP-2 or ERK signaling pathway. Aloe-emodin may have potential future applications for the treatment of growth disorders.

No MeSH data available.


Related in: MedlinePlus

Expression of chondrogenic marker genes in ATDC5 cells treated with aloe-emodin. ATDC5 cells were treated with 2.5 μM, 5 μM, 10 μM aloe-emodin for 21 days. The mRNA levels of different chondrogenic marker molecules, including Aggrecan, BSP and Runx2 were determined by RT-PCR analysis and compared to the levels of GAPDH (A). ATDC5 cells were treated with 5 μM aloe-emodin or 10 μg/ml insulin were incubated for the indicated time periods (B). Relative expression of type II collagen, Aggrecan, type I collagen, type X collagen, Runx2, BSP and Osterix were observed by RT-PCR analysis and compared to GAPDH.
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f4-bt-24-395: Expression of chondrogenic marker genes in ATDC5 cells treated with aloe-emodin. ATDC5 cells were treated with 2.5 μM, 5 μM, 10 μM aloe-emodin for 21 days. The mRNA levels of different chondrogenic marker molecules, including Aggrecan, BSP and Runx2 were determined by RT-PCR analysis and compared to the levels of GAPDH (A). ATDC5 cells were treated with 5 μM aloe-emodin or 10 μg/ml insulin were incubated for the indicated time periods (B). Relative expression of type II collagen, Aggrecan, type I collagen, type X collagen, Runx2, BSP and Osterix were observed by RT-PCR analysis and compared to GAPDH.

Mentions: Aloe-emodin significantly increased the expression of chondrogenic marker genes such as Aggrecan, bone sialoprotein (BSP) and Runx2 (Fig. 4A). To determine the stimulatory effect of aloe-emodin on chondrogenesis, the expressions of type II collagen, Aggrecan, type I collagen, type X collagen, Runx2, BSP, and Osterix were measured using real-time PCR (Fig. 4B). ATDC5 cells were treated with 5 μM aloe-emodin at different time intervals. The expressions of type II collagen, Aggrecan, type I collagen, and Osterix were significantly increased at 14 days. Similarly, type X collagen and Runx2 were also increased at 14 days and still in the stable state observation at 21 days. The expression pattern was similar to that of insulin, which was used as a positive control. In addition, BSP had significant expression at 21 days.These results suggest that aloe-emodin induces chondrogenic differentiation in ATDC5 cells.


Aloe-Emodin Induces Chondrogenic Differentiation of ATDC5 Cells via MAP Kinases and BMP-2 Signaling Pathways.

Yang M, Li L, Heo SM, Soh Y - Biomol Ther (Seoul) (2016)

Expression of chondrogenic marker genes in ATDC5 cells treated with aloe-emodin. ATDC5 cells were treated with 2.5 μM, 5 μM, 10 μM aloe-emodin for 21 days. The mRNA levels of different chondrogenic marker molecules, including Aggrecan, BSP and Runx2 were determined by RT-PCR analysis and compared to the levels of GAPDH (A). ATDC5 cells were treated with 5 μM aloe-emodin or 10 μg/ml insulin were incubated for the indicated time periods (B). Relative expression of type II collagen, Aggrecan, type I collagen, type X collagen, Runx2, BSP and Osterix were observed by RT-PCR analysis and compared to GAPDH.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4930283&req=5

f4-bt-24-395: Expression of chondrogenic marker genes in ATDC5 cells treated with aloe-emodin. ATDC5 cells were treated with 2.5 μM, 5 μM, 10 μM aloe-emodin for 21 days. The mRNA levels of different chondrogenic marker molecules, including Aggrecan, BSP and Runx2 were determined by RT-PCR analysis and compared to the levels of GAPDH (A). ATDC5 cells were treated with 5 μM aloe-emodin or 10 μg/ml insulin were incubated for the indicated time periods (B). Relative expression of type II collagen, Aggrecan, type I collagen, type X collagen, Runx2, BSP and Osterix were observed by RT-PCR analysis and compared to GAPDH.
Mentions: Aloe-emodin significantly increased the expression of chondrogenic marker genes such as Aggrecan, bone sialoprotein (BSP) and Runx2 (Fig. 4A). To determine the stimulatory effect of aloe-emodin on chondrogenesis, the expressions of type II collagen, Aggrecan, type I collagen, type X collagen, Runx2, BSP, and Osterix were measured using real-time PCR (Fig. 4B). ATDC5 cells were treated with 5 μM aloe-emodin at different time intervals. The expressions of type II collagen, Aggrecan, type I collagen, and Osterix were significantly increased at 14 days. Similarly, type X collagen and Runx2 were also increased at 14 days and still in the stable state observation at 21 days. The expression pattern was similar to that of insulin, which was used as a positive control. In addition, BSP had significant expression at 21 days.These results suggest that aloe-emodin induces chondrogenic differentiation in ATDC5 cells.

Bottom Line: Furthermore, examination of the MAPK signaling pathway showed that aloe-emodin increased the activation of extracellular signal-regulated kinase (ERK), but had no effect on p38 and c-jun N-terminal kinase (JNK).Thus, these results showed that aloe-emodin exhibited chodromodulating effects via the BMP-2 or ERK signaling pathway.Aloe-emodin may have potential future applications for the treatment of growth disorders.

View Article: PubMed Central - PubMed

Affiliation: Department of Periodontology, School of Dentistry, Chonbuk National University, Jeonju 54896, Republic of Korea.

ABSTRACT
Endochondral bone formation is the process by which mesenchymal cells condense into chondrocytes, which are ultimately responsible for new bone formation. The processes of chondrogenic differentiation and hypertrophy are critical for bone formation and are therefore highly regulated. The present study was designed to investigate the effect of aloe-emodin on chondrogenic differentiation in clonal mouse chondrogenic ATDC5 cells. Aloe-emodin treatment stimulated the accumulation of cartilage nodules in a dose-dependent manner. ATDC5 cells were treated with aloe-emodin and stained with alcian blue. Compared with the control cells, the ATDC5 cells showed more intense alcian blue staining. This finding suggested that aloe-emodin induced the synthesis of matrix proteoglycans and increased the activity of alkaline phosphatase. Aloe-emodin also enhanced the expressions of chondrogenic marker genes such as collagen II, collagen X, BSP and RunX2 in a time-dependent manner. Furthermore, examination of the MAPK signaling pathway showed that aloe-emodin increased the activation of extracellular signal-regulated kinase (ERK), but had no effect on p38 and c-jun N-terminal kinase (JNK). Aloe-emodin also enhanced the protein expression of BMP-2 in a time-dependent manner. Thus, these results showed that aloe-emodin exhibited chodromodulating effects via the BMP-2 or ERK signaling pathway. Aloe-emodin may have potential future applications for the treatment of growth disorders.

No MeSH data available.


Related in: MedlinePlus