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A vanillic acid inducible expression system for Trypanosoma brucei.

Sunter JD - Mol. Biochem. Parasitol. (2016)

Bottom Line: Reverse genetics in Trypanosoma brucei is dependent on the tetracycline inducible system for the precise control over the expression of both genes and dsRNA.Another independent inducible system for trypanosomes would enable the control of the activities of two different genes in the same cell, providing greater experimental sophistication.Here, I describe the development of the vanillic acid based inducible expression system for T. brucei, which operates independently of, and can be used in parallel with the tetracycline inducible system.

View Article: PubMed Central - PubMed

Affiliation: Sir William Dunn School of Pathology, University of Oxford, South Parks Road, OX1 3RE, UK. Electronic address: jack.sunter@path.ox.ac.uk.

No MeSH data available.


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A vanillic acid inducible expression system for Trypanosoma brucei.

Sunter JD - Mol. Biochem. Parasitol. (2016)

© Copyright Policy - CC BY
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4920640&req=5

Bottom Line: Reverse genetics in Trypanosoma brucei is dependent on the tetracycline inducible system for the precise control over the expression of both genes and dsRNA.Another independent inducible system for trypanosomes would enable the control of the activities of two different genes in the same cell, providing greater experimental sophistication.Here, I describe the development of the vanillic acid based inducible expression system for T. brucei, which operates independently of, and can be used in parallel with the tetracycline inducible system.

View Article: PubMed Central - PubMed

Affiliation: Sir William Dunn School of Pathology, University of Oxford, South Parks Road, OX1 3RE, UK. Electronic address: jack.sunter@path.ox.ac.uk.

No MeSH data available.