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Nitric oxide and asymmetric dimethyl arginine (ADMA) levels in an experimental hydronephrotic kidney caused by unilateral partial ureteral obstruction.

Alan C, Kurt HA, Topaloğlu N, Ersay AR, Cakir DU, Başturk G - Int Braz J Urol (2016 May-Jun)

Bottom Line: In the experimental group tissue SOD and blood ADMA levels were higher than the control and sham groups (p<0.05) while tissue NO and plasma NO values were lower than in the sham and control groups (p<0.05).Oxidative stress and disruption of NO synthesis play an important role in renal function and histopathological changes after obstructive renal disease.To prevent renal complications developing after obstructive nephropathy we believe that a new strategy may be research on reducing ADMA.

View Article: PubMed Central - PubMed

Affiliation: Department of Urology, Medical Faculty, Canakkale Onsekiz Mart University, Turkey.

ABSTRACT

Aim: Our aim is to measure asymmetric dimethyl arginine and nitric oxide levels in rats with induced unilateral acute ureteral obstruction to research the effects on the kidney.

Material and methods: The study included 21 adolescent (average age 6 weeks) Sprague-Dawley male rats weighing between 240-290g divided at random into 3 groups. Group-1: Control group (n=6): underwent no procedures. Group-2: Sham group (n=6): underwent the same procedures as the experimental group without ureter and psoas muscle dissection. Group-3: Group with induced partial unilateral ureteral obstruction (n=9). All rats were sacrificed after 12 weeks. Superoxide dismutase enzyme activity and nitrite and nitrate salt levels were measured in renal tissue. Plasma nitrite-nitrate and ADMA levels were examined.

Results: In the experimental group histopathological changes observed included renal pelvis dilatation, flattened papillae, sclerotic glomerulus and fibrosis. In the experimental group tissue SOD and blood ADMA levels were higher than the control and sham groups (p<0.05) while tissue NO and plasma NO values were lower than in the sham and control groups (p<0.05).

Conclusion: Oxidative stress and disruption of NO synthesis play an important role in renal function and histopathological changes after obstructive renal disease. To prevent renal complications developing after obstructive nephropathy we believe that a new strategy may be research on reducing ADMA.

No MeSH data available.


Related in: MedlinePlus

SOD values measured in tissue samples of the groups (μmoL/g tissue).
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f02: SOD values measured in tissue samples of the groups (μmoL/g tissue).

Mentions: Linked to increased oxidative stress the tissue SOD values, the enzyme with primary antioxidant properties, are summarized in Table-1. In the experimental group with induced PUUO, tissue levels of SOD were higher than in the sham and control groups (p=0.001) (Figure-2). Comparing the groups, there were differences between the experimental and control groups (p=0.001) and between the experimental and sham groups (p=0.003) (Table-1).


Nitric oxide and asymmetric dimethyl arginine (ADMA) levels in an experimental hydronephrotic kidney caused by unilateral partial ureteral obstruction.

Alan C, Kurt HA, Topaloğlu N, Ersay AR, Cakir DU, Başturk G - Int Braz J Urol (2016 May-Jun)

SOD values measured in tissue samples of the groups (μmoL/g tissue).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4920583&req=5

f02: SOD values measured in tissue samples of the groups (μmoL/g tissue).
Mentions: Linked to increased oxidative stress the tissue SOD values, the enzyme with primary antioxidant properties, are summarized in Table-1. In the experimental group with induced PUUO, tissue levels of SOD were higher than in the sham and control groups (p=0.001) (Figure-2). Comparing the groups, there were differences between the experimental and control groups (p=0.001) and between the experimental and sham groups (p=0.003) (Table-1).

Bottom Line: In the experimental group tissue SOD and blood ADMA levels were higher than the control and sham groups (p<0.05) while tissue NO and plasma NO values were lower than in the sham and control groups (p<0.05).Oxidative stress and disruption of NO synthesis play an important role in renal function and histopathological changes after obstructive renal disease.To prevent renal complications developing after obstructive nephropathy we believe that a new strategy may be research on reducing ADMA.

View Article: PubMed Central - PubMed

Affiliation: Department of Urology, Medical Faculty, Canakkale Onsekiz Mart University, Turkey.

ABSTRACT

Aim: Our aim is to measure asymmetric dimethyl arginine and nitric oxide levels in rats with induced unilateral acute ureteral obstruction to research the effects on the kidney.

Material and methods: The study included 21 adolescent (average age 6 weeks) Sprague-Dawley male rats weighing between 240-290g divided at random into 3 groups. Group-1: Control group (n=6): underwent no procedures. Group-2: Sham group (n=6): underwent the same procedures as the experimental group without ureter and psoas muscle dissection. Group-3: Group with induced partial unilateral ureteral obstruction (n=9). All rats were sacrificed after 12 weeks. Superoxide dismutase enzyme activity and nitrite and nitrate salt levels were measured in renal tissue. Plasma nitrite-nitrate and ADMA levels were examined.

Results: In the experimental group histopathological changes observed included renal pelvis dilatation, flattened papillae, sclerotic glomerulus and fibrosis. In the experimental group tissue SOD and blood ADMA levels were higher than the control and sham groups (p<0.05) while tissue NO and plasma NO values were lower than in the sham and control groups (p<0.05).

Conclusion: Oxidative stress and disruption of NO synthesis play an important role in renal function and histopathological changes after obstructive renal disease. To prevent renal complications developing after obstructive nephropathy we believe that a new strategy may be research on reducing ADMA.

No MeSH data available.


Related in: MedlinePlus