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Nitric oxide and asymmetric dimethyl arginine (ADMA) levels in an experimental hydronephrotic kidney caused by unilateral partial ureteral obstruction.

Alan C, Kurt HA, Topaloğlu N, Ersay AR, Cakir DU, Başturk G - Int Braz J Urol (2016 May-Jun)

Bottom Line: In the experimental group tissue SOD and blood ADMA levels were higher than the control and sham groups (p<0.05) while tissue NO and plasma NO values were lower than in the sham and control groups (p<0.05).Oxidative stress and disruption of NO synthesis play an important role in renal function and histopathological changes after obstructive renal disease.To prevent renal complications developing after obstructive nephropathy we believe that a new strategy may be research on reducing ADMA.

View Article: PubMed Central - PubMed

Affiliation: Department of Urology, Medical Faculty, Canakkale Onsekiz Mart University, Turkey.

ABSTRACT

Aim: Our aim is to measure asymmetric dimethyl arginine and nitric oxide levels in rats with induced unilateral acute ureteral obstruction to research the effects on the kidney.

Material and methods: The study included 21 adolescent (average age 6 weeks) Sprague-Dawley male rats weighing between 240-290g divided at random into 3 groups. Group-1: Control group (n=6): underwent no procedures. Group-2: Sham group (n=6): underwent the same procedures as the experimental group without ureter and psoas muscle dissection. Group-3: Group with induced partial unilateral ureteral obstruction (n=9). All rats were sacrificed after 12 weeks. Superoxide dismutase enzyme activity and nitrite and nitrate salt levels were measured in renal tissue. Plasma nitrite-nitrate and ADMA levels were examined.

Results: In the experimental group histopathological changes observed included renal pelvis dilatation, flattened papillae, sclerotic glomerulus and fibrosis. In the experimental group tissue SOD and blood ADMA levels were higher than the control and sham groups (p<0.05) while tissue NO and plasma NO values were lower than in the sham and control groups (p<0.05).

Conclusion: Oxidative stress and disruption of NO synthesis play an important role in renal function and histopathological changes after obstructive renal disease. To prevent renal complications developing after obstructive nephropathy we believe that a new strategy may be research on reducing ADMA.

No MeSH data available.


Related in: MedlinePlus

a) Macroscopic view of kidney tissue of experimental group; b) Macroscopic view of kidney tissue of control group; c) Hematoxilin and eosin (H&E) stained histopathological images of kidney tissue of experimental group; renal pelvis dilatation and flattening; d) Hematoxilin and eosin stained histopathological images of kidney tissue of control group; there was no pathological findings on renal papilla, cortex and medulla.
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f01: a) Macroscopic view of kidney tissue of experimental group; b) Macroscopic view of kidney tissue of control group; c) Hematoxilin and eosin (H&E) stained histopathological images of kidney tissue of experimental group; renal pelvis dilatation and flattening; d) Hematoxilin and eosin stained histopathological images of kidney tissue of control group; there was no pathological findings on renal papilla, cortex and medulla.

Mentions: Partial unilateral ureteral obstruction (PUUO) model: A 2cm incision was performed at the anterior abdomen region. Skin above the linea alba, subdermis, abdominal anterior wall and peritoneum was incised to reach abdominal cavity and the left kidney was located. Fat and connective tissues were dissected to reveal the left ureter and psoas muscle. For PUUO rats, similar to the technique of Ulm and Miller (12), the psoas muscle under the left ureter was longitudinally dissected to form a groove (~15mm) and a small part of the left ureter was placed into the groove. Later the edges of the psoas muscle were fixed above the ureter with 5/0 silk suture. Thus the ureter was enclosed in a tunnel (Figure-1). In the sham model rats after the left ureter and psoas muscle were located, the procedure was completed without ureter and psoas muscle dissection. Later 3/0 catgut and 3/0 silk suture were used to close the abdomen in 2 layers. All rats were sacrificed after 12 weeks.


Nitric oxide and asymmetric dimethyl arginine (ADMA) levels in an experimental hydronephrotic kidney caused by unilateral partial ureteral obstruction.

Alan C, Kurt HA, Topaloğlu N, Ersay AR, Cakir DU, Başturk G - Int Braz J Urol (2016 May-Jun)

a) Macroscopic view of kidney tissue of experimental group; b) Macroscopic view of kidney tissue of control group; c) Hematoxilin and eosin (H&E) stained histopathological images of kidney tissue of experimental group; renal pelvis dilatation and flattening; d) Hematoxilin and eosin stained histopathological images of kidney tissue of control group; there was no pathological findings on renal papilla, cortex and medulla.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4920583&req=5

f01: a) Macroscopic view of kidney tissue of experimental group; b) Macroscopic view of kidney tissue of control group; c) Hematoxilin and eosin (H&E) stained histopathological images of kidney tissue of experimental group; renal pelvis dilatation and flattening; d) Hematoxilin and eosin stained histopathological images of kidney tissue of control group; there was no pathological findings on renal papilla, cortex and medulla.
Mentions: Partial unilateral ureteral obstruction (PUUO) model: A 2cm incision was performed at the anterior abdomen region. Skin above the linea alba, subdermis, abdominal anterior wall and peritoneum was incised to reach abdominal cavity and the left kidney was located. Fat and connective tissues were dissected to reveal the left ureter and psoas muscle. For PUUO rats, similar to the technique of Ulm and Miller (12), the psoas muscle under the left ureter was longitudinally dissected to form a groove (~15mm) and a small part of the left ureter was placed into the groove. Later the edges of the psoas muscle were fixed above the ureter with 5/0 silk suture. Thus the ureter was enclosed in a tunnel (Figure-1). In the sham model rats after the left ureter and psoas muscle were located, the procedure was completed without ureter and psoas muscle dissection. Later 3/0 catgut and 3/0 silk suture were used to close the abdomen in 2 layers. All rats were sacrificed after 12 weeks.

Bottom Line: In the experimental group tissue SOD and blood ADMA levels were higher than the control and sham groups (p<0.05) while tissue NO and plasma NO values were lower than in the sham and control groups (p<0.05).Oxidative stress and disruption of NO synthesis play an important role in renal function and histopathological changes after obstructive renal disease.To prevent renal complications developing after obstructive nephropathy we believe that a new strategy may be research on reducing ADMA.

View Article: PubMed Central - PubMed

Affiliation: Department of Urology, Medical Faculty, Canakkale Onsekiz Mart University, Turkey.

ABSTRACT

Aim: Our aim is to measure asymmetric dimethyl arginine and nitric oxide levels in rats with induced unilateral acute ureteral obstruction to research the effects on the kidney.

Material and methods: The study included 21 adolescent (average age 6 weeks) Sprague-Dawley male rats weighing between 240-290g divided at random into 3 groups. Group-1: Control group (n=6): underwent no procedures. Group-2: Sham group (n=6): underwent the same procedures as the experimental group without ureter and psoas muscle dissection. Group-3: Group with induced partial unilateral ureteral obstruction (n=9). All rats were sacrificed after 12 weeks. Superoxide dismutase enzyme activity and nitrite and nitrate salt levels were measured in renal tissue. Plasma nitrite-nitrate and ADMA levels were examined.

Results: In the experimental group histopathological changes observed included renal pelvis dilatation, flattened papillae, sclerotic glomerulus and fibrosis. In the experimental group tissue SOD and blood ADMA levels were higher than the control and sham groups (p<0.05) while tissue NO and plasma NO values were lower than in the sham and control groups (p<0.05).

Conclusion: Oxidative stress and disruption of NO synthesis play an important role in renal function and histopathological changes after obstructive renal disease. To prevent renal complications developing after obstructive nephropathy we believe that a new strategy may be research on reducing ADMA.

No MeSH data available.


Related in: MedlinePlus