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Host inflammatory response to polypropylene implants: insights from a quantitative immunohistochemical and birefringence analysis in a rat subcutaneous model.

Prudente A, Favaro WJ, Latuf P, Riccetto CL - Int Braz J Urol (2016 May-Jun)

Bottom Line: After 30 days, a reduction of IL-1 (p=0.010) and TNF (p=0.016) for PP and of IL-1 (p=0.010) for sham were observed.Moreover, area of MMP-2 immunoreactivity decreased over time for PP group (p=0.018).The implantation of monofilament and macroporous polypropylene in the subcutaneous of rats resulted in increased inflammatory activity and higher TNF production in the early post implant phase.

View Article: PubMed Central - PubMed

Affiliation: Faculdade de Ciências Médicas da Universidade de Campinas, Campinas, SP, Brasil.

ABSTRACT

Objectives: To describe acute and sub acute aspects of histological and immunohistochemical response to PP implant in a rat subcutaneous model based on objective methods.

Materials and methods: Thirty rats had a PP mesh subcutaneously implanted and the same dissection on the other side of abdomen but without mesh (sham). The animals were euthanized after 4 and 30 days. Six slides were prepared using the tissue removed: one stained with hematoxylin-eosin (inflammation assessment); one unstained (birefringence evaluation) and four slides for immunohistochemical processing: IL-1 and TNF-α (pro-inflammatory cytokines), MMP-2 (collagen metabolism) and CD-31 (angiogenesis). The area of inflammation, the birefringence index, the area of immunoreactivity and the number of vessels were objectively measured.

Results: A larger area of inflammatory reaction was observed in PP compared to sham on the 4th and on the 30th day (p=0.0002). After 4 days, PP presented higher TNF (p=0.0001) immunoreactivity than sham and no differences were observed in MMP-2 (p=0.06) and IL-1 (p=0.08). After 30 days, a reduction of IL-1 (p=0.010) and TNF (p=0.016) for PP and of IL-1 (p=0.010) for sham were observed. Moreover, area of MMP-2 immunoreactivity decreased over time for PP group (p=0.018). Birefringence index and vessel counting showed no differences between PP and sham (p=0.27 and p=0.58, respectively).

Conclusions: The implantation of monofilament and macroporous polypropylene in the subcutaneous of rats resulted in increased inflammatory activity and higher TNF production in the early post implant phase. After 30 days, PP has similar cytokines immunoreactivity, vessel density and extracellular matrix organization.

No MeSH data available.


Related in: MedlinePlus

Example of MMP2 immunoreactivity in an implant sample. Blank rounded area indicates the PP filament. (A) Before software selection. (B) After selection, note MMP2 immunoreactivity colored in blue (200x).
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f03: Example of MMP2 immunoreactivity in an implant sample. Blank rounded area indicates the PP filament. (A) Before software selection. (B) After selection, note MMP2 immunoreactivity colored in blue (200x).

Mentions: A Primo StarTM Zeiss microscope (Carl Zeiss Microscopy, Jena, Germany) was used for histological evaluation. The entire slide was scanned using a 200x magnification (400x for vessel density), and three fields for each slide were randomly selected for subsequent image acquisition using a Zeiss AxioCam camera ICC1TM. Objective analysis of immunoreaction (percentage area of immunoreactivity and vessel density) was carried out with AxioVision V 4.8.0.0 Software Microscope (Karl Zeiss-Germany) (Figure-3).


Host inflammatory response to polypropylene implants: insights from a quantitative immunohistochemical and birefringence analysis in a rat subcutaneous model.

Prudente A, Favaro WJ, Latuf P, Riccetto CL - Int Braz J Urol (2016 May-Jun)

Example of MMP2 immunoreactivity in an implant sample. Blank rounded area indicates the PP filament. (A) Before software selection. (B) After selection, note MMP2 immunoreactivity colored in blue (200x).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4920579&req=5

f03: Example of MMP2 immunoreactivity in an implant sample. Blank rounded area indicates the PP filament. (A) Before software selection. (B) After selection, note MMP2 immunoreactivity colored in blue (200x).
Mentions: A Primo StarTM Zeiss microscope (Carl Zeiss Microscopy, Jena, Germany) was used for histological evaluation. The entire slide was scanned using a 200x magnification (400x for vessel density), and three fields for each slide were randomly selected for subsequent image acquisition using a Zeiss AxioCam camera ICC1TM. Objective analysis of immunoreaction (percentage area of immunoreactivity and vessel density) was carried out with AxioVision V 4.8.0.0 Software Microscope (Karl Zeiss-Germany) (Figure-3).

Bottom Line: After 30 days, a reduction of IL-1 (p=0.010) and TNF (p=0.016) for PP and of IL-1 (p=0.010) for sham were observed.Moreover, area of MMP-2 immunoreactivity decreased over time for PP group (p=0.018).The implantation of monofilament and macroporous polypropylene in the subcutaneous of rats resulted in increased inflammatory activity and higher TNF production in the early post implant phase.

View Article: PubMed Central - PubMed

Affiliation: Faculdade de Ciências Médicas da Universidade de Campinas, Campinas, SP, Brasil.

ABSTRACT

Objectives: To describe acute and sub acute aspects of histological and immunohistochemical response to PP implant in a rat subcutaneous model based on objective methods.

Materials and methods: Thirty rats had a PP mesh subcutaneously implanted and the same dissection on the other side of abdomen but without mesh (sham). The animals were euthanized after 4 and 30 days. Six slides were prepared using the tissue removed: one stained with hematoxylin-eosin (inflammation assessment); one unstained (birefringence evaluation) and four slides for immunohistochemical processing: IL-1 and TNF-α (pro-inflammatory cytokines), MMP-2 (collagen metabolism) and CD-31 (angiogenesis). The area of inflammation, the birefringence index, the area of immunoreactivity and the number of vessels were objectively measured.

Results: A larger area of inflammatory reaction was observed in PP compared to sham on the 4th and on the 30th day (p=0.0002). After 4 days, PP presented higher TNF (p=0.0001) immunoreactivity than sham and no differences were observed in MMP-2 (p=0.06) and IL-1 (p=0.08). After 30 days, a reduction of IL-1 (p=0.010) and TNF (p=0.016) for PP and of IL-1 (p=0.010) for sham were observed. Moreover, area of MMP-2 immunoreactivity decreased over time for PP group (p=0.018). Birefringence index and vessel counting showed no differences between PP and sham (p=0.27 and p=0.58, respectively).

Conclusions: The implantation of monofilament and macroporous polypropylene in the subcutaneous of rats resulted in increased inflammatory activity and higher TNF production in the early post implant phase. After 30 days, PP has similar cytokines immunoreactivity, vessel density and extracellular matrix organization.

No MeSH data available.


Related in: MedlinePlus