Limits...
miR156a Mimic Represses the Epithelial-Mesenchymal Transition of Human Nasopharyngeal Cancer Cells by Targeting Junctional Adhesion Molecule A.

Tian Y, Cai L, Tian Y, Tu Y, Qiu H, Xie G, Huang D, Zheng R, Zhang W - PLoS ONE (2016)

Bottom Line: We demonstrated that a total of 84 conserved miRNAs and 184 putative novel miRNAs were found in broccoli by sequencing technology.Knockdown of JAMA was consistent with the effects of miR156a mimic on the EMT of NPC, and the up-regulation of JAMA could partially restore EMT repressed by miR156a mimic.In conclusion, these results indicate that the miR156a mimic inhibits the EMT of NPC cells by targeting the 3' UTR of JAMA.

View Article: PubMed Central - PubMed

Affiliation: Department of Radiation Oncology, Cancer Center of Guangzhou Medical University, Guangzhou, Guangdong Province, People's Republic of China.

ABSTRACT
MicroRNAs (miRNAs) have been documented as having an important role in the development of cancer. Broccoli is very popular in large groups of the population and has anticancer properties. Junctional adhesion molecule A (JAMA) is preferentially concentrated at tight junctions and influences cell morphology and migration. Epithelial-mesenchymal transition (EMT) is a developmental program associated with cancer progression and metastasis. In this study we aimed to investigate the role of miRNAs from broccoli in human nasopharyngeal cancer (NPC). We demonstrated that a total of 84 conserved miRNAs and 184 putative novel miRNAs were found in broccoli by sequencing technology. Among these, miR156a was expressed the most. In addition, synthetic miR156a mimic inhibited the EMT of NPC cells in vitro. Furthermore, it was confirmed that JAMA was the target of miR156a mimic as validated by 3' UTR luciferase reporter assays and western blotting. Knockdown of JAMA was consistent with the effects of miR156a mimic on the EMT of NPC, and the up-regulation of JAMA could partially restore EMT repressed by miR156a mimic. In conclusion, these results indicate that the miR156a mimic inhibits the EMT of NPC cells by targeting the 3' UTR of JAMA. These miRNA profiles of broccoli provide a fundamental basis for further research. Moreover, the discovery of miR156a may have clinical implications for the treatment of patients with NPC.

No MeSH data available.


Related in: MedlinePlus

Broccoli miR156a mimic represses the EMT phenotype of NPC cells in vitro.Cells are harvested 24 hours after transfection. (A) The invasive properties of the CNE2 (A), HONE1 (B) and C666-1 (C) cells were measured by transwell and Boyden chamber assays. A wound-healing assay of CNE2 (D) and HONE1 (E) cells was performed. The total distance migrated by wounded cells is expressed as a percentage of the initial distance (F). (C) The expressions of epithelial (E-cadherin and α-catenin) and mesenchymal markers (vimentin and Fibronectin) in CNE2 (G) and C666-1 (H) were measured by western blotting. Data are presented as mean ± SD (n = 3). Significant differences between the negative controls and miRNA156a mimics indicated by *P < 0.05 and **P < 0.01.
© Copyright Policy
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4920421&req=5

pone.0157686.g002: Broccoli miR156a mimic represses the EMT phenotype of NPC cells in vitro.Cells are harvested 24 hours after transfection. (A) The invasive properties of the CNE2 (A), HONE1 (B) and C666-1 (C) cells were measured by transwell and Boyden chamber assays. A wound-healing assay of CNE2 (D) and HONE1 (E) cells was performed. The total distance migrated by wounded cells is expressed as a percentage of the initial distance (F). (C) The expressions of epithelial (E-cadherin and α-catenin) and mesenchymal markers (vimentin and Fibronectin) in CNE2 (G) and C666-1 (H) were measured by western blotting. Data are presented as mean ± SD (n = 3). Significant differences between the negative controls and miRNA156a mimics indicated by *P < 0.05 and **P < 0.01.

Mentions: To address the question whether miR156a from broccoli exhibits anticancer activity, we conducted an analysis of miR156a mimic on the EMT of NPC in vitro. The cellular migration and invasive activities of NPC cells upon stimulation with miR156a mimic were investigated by transwell and Boyden assays. We found that miR156a mimic significantly suppressed the invasion of CNE2, HONE1 and C666-1 cells in Boyden assays and reduced migration in transwell assays (Fig 2A, 2B and 2C). Moreover, the migration index decreased significantly in CNE2 and HONE1 cells when miR156a mimics were transfected (Fig 2D, 2E and 2F). In miR156a mimic-transfected cell lines, western blotting revealed an increase in expression levels of α-catenin and E-cadherin, and a decrease in expression levels of the mesenchymal cell markers fibronectin and vimentin (Fig 2G and 2H). These results indicate that miR156a mimic represses the EMT of NPC cells in vitro.


miR156a Mimic Represses the Epithelial-Mesenchymal Transition of Human Nasopharyngeal Cancer Cells by Targeting Junctional Adhesion Molecule A.

Tian Y, Cai L, Tian Y, Tu Y, Qiu H, Xie G, Huang D, Zheng R, Zhang W - PLoS ONE (2016)

Broccoli miR156a mimic represses the EMT phenotype of NPC cells in vitro.Cells are harvested 24 hours after transfection. (A) The invasive properties of the CNE2 (A), HONE1 (B) and C666-1 (C) cells were measured by transwell and Boyden chamber assays. A wound-healing assay of CNE2 (D) and HONE1 (E) cells was performed. The total distance migrated by wounded cells is expressed as a percentage of the initial distance (F). (C) The expressions of epithelial (E-cadherin and α-catenin) and mesenchymal markers (vimentin and Fibronectin) in CNE2 (G) and C666-1 (H) were measured by western blotting. Data are presented as mean ± SD (n = 3). Significant differences between the negative controls and miRNA156a mimics indicated by *P < 0.05 and **P < 0.01.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4920421&req=5

pone.0157686.g002: Broccoli miR156a mimic represses the EMT phenotype of NPC cells in vitro.Cells are harvested 24 hours after transfection. (A) The invasive properties of the CNE2 (A), HONE1 (B) and C666-1 (C) cells were measured by transwell and Boyden chamber assays. A wound-healing assay of CNE2 (D) and HONE1 (E) cells was performed. The total distance migrated by wounded cells is expressed as a percentage of the initial distance (F). (C) The expressions of epithelial (E-cadherin and α-catenin) and mesenchymal markers (vimentin and Fibronectin) in CNE2 (G) and C666-1 (H) were measured by western blotting. Data are presented as mean ± SD (n = 3). Significant differences between the negative controls and miRNA156a mimics indicated by *P < 0.05 and **P < 0.01.
Mentions: To address the question whether miR156a from broccoli exhibits anticancer activity, we conducted an analysis of miR156a mimic on the EMT of NPC in vitro. The cellular migration and invasive activities of NPC cells upon stimulation with miR156a mimic were investigated by transwell and Boyden assays. We found that miR156a mimic significantly suppressed the invasion of CNE2, HONE1 and C666-1 cells in Boyden assays and reduced migration in transwell assays (Fig 2A, 2B and 2C). Moreover, the migration index decreased significantly in CNE2 and HONE1 cells when miR156a mimics were transfected (Fig 2D, 2E and 2F). In miR156a mimic-transfected cell lines, western blotting revealed an increase in expression levels of α-catenin and E-cadherin, and a decrease in expression levels of the mesenchymal cell markers fibronectin and vimentin (Fig 2G and 2H). These results indicate that miR156a mimic represses the EMT of NPC cells in vitro.

Bottom Line: We demonstrated that a total of 84 conserved miRNAs and 184 putative novel miRNAs were found in broccoli by sequencing technology.Knockdown of JAMA was consistent with the effects of miR156a mimic on the EMT of NPC, and the up-regulation of JAMA could partially restore EMT repressed by miR156a mimic.In conclusion, these results indicate that the miR156a mimic inhibits the EMT of NPC cells by targeting the 3' UTR of JAMA.

View Article: PubMed Central - PubMed

Affiliation: Department of Radiation Oncology, Cancer Center of Guangzhou Medical University, Guangzhou, Guangdong Province, People's Republic of China.

ABSTRACT
MicroRNAs (miRNAs) have been documented as having an important role in the development of cancer. Broccoli is very popular in large groups of the population and has anticancer properties. Junctional adhesion molecule A (JAMA) is preferentially concentrated at tight junctions and influences cell morphology and migration. Epithelial-mesenchymal transition (EMT) is a developmental program associated with cancer progression and metastasis. In this study we aimed to investigate the role of miRNAs from broccoli in human nasopharyngeal cancer (NPC). We demonstrated that a total of 84 conserved miRNAs and 184 putative novel miRNAs were found in broccoli by sequencing technology. Among these, miR156a was expressed the most. In addition, synthetic miR156a mimic inhibited the EMT of NPC cells in vitro. Furthermore, it was confirmed that JAMA was the target of miR156a mimic as validated by 3' UTR luciferase reporter assays and western blotting. Knockdown of JAMA was consistent with the effects of miR156a mimic on the EMT of NPC, and the up-regulation of JAMA could partially restore EMT repressed by miR156a mimic. In conclusion, these results indicate that the miR156a mimic inhibits the EMT of NPC cells by targeting the 3' UTR of JAMA. These miRNA profiles of broccoli provide a fundamental basis for further research. Moreover, the discovery of miR156a may have clinical implications for the treatment of patients with NPC.

No MeSH data available.


Related in: MedlinePlus