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TAF4b Regulates Oocyte-Specific Genes Essential for Meiosis.

Grive KJ, Gustafson EA, Seymour KA, Baddoo M, Schorl C, Golnoski K, Rajkovic A, Brodsky AS, Freiman RN - PLoS Genet. (2016)

Bottom Line: To further explore the potential mechanism of TAF4b in promoting ovarian follicle development, we analyzed global gene expression at multiple time points in the human fetal ovary.This computational analysis revealed coordinate expression of human TAF4B and critical regulators and effectors of meiosis I including SYCP3, YBX2, STAG3, and DAZL.Accordingly, TAF4b occupies the proximal promoters of many essential meiosis and oogenesis regulators, including Stra8, Dazl, Figla, and Nobox, and is required for their proper expression.

View Article: PubMed Central - PubMed

Affiliation: MCB Graduate Program, Brown University, Providence, Rhode Island, United States of America.

ABSTRACT
TAF4b is a gonadal-enriched subunit of the general transcription factor TFIID that is implicated in promoting healthy ovarian aging and female fertility in mice and humans. To further explore the potential mechanism of TAF4b in promoting ovarian follicle development, we analyzed global gene expression at multiple time points in the human fetal ovary. This computational analysis revealed coordinate expression of human TAF4B and critical regulators and effectors of meiosis I including SYCP3, YBX2, STAG3, and DAZL. To address the functional relevance of this analysis, we turned to the embryonic Taf4b-deficient mouse ovary where, for the first time, we demonstrate, severe deficits in prophase I progression as well as asynapsis in Taf4b-deficient oocytes. Accordingly, TAF4b occupies the proximal promoters of many essential meiosis and oogenesis regulators, including Stra8, Dazl, Figla, and Nobox, and is required for their proper expression. These data reveal a novel TAF4b function in regulating a meiotic gene expression program in early mouse oogenesis, and support the existence of a highly conserved TAF4b-dependent gene regulatory network promoting early oocyte development in both mice and women.

No MeSH data available.


Related in: MedlinePlus

Model of TAF4b promoting a critical meiosis and oogenesis gene regulatory network: Data from chromatin immunoprecipitation experiments demonstrates that TAF4b occupies the proximal promoters of Dazl, Stra8, Figlα, and Nobox, ultimately resulting in their expression.Proper expression of these essential regulators facilitates expression of downstream meiosis and oogenesis genes, finally leading to the development of a healthy primordial follicle pool. Red arrows represent direct transcriptional regulation, gray arrows represent post-transcriptional regulation, and dashed lines indicate putative mechanisms.
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pgen.1006128.g008: Model of TAF4b promoting a critical meiosis and oogenesis gene regulatory network: Data from chromatin immunoprecipitation experiments demonstrates that TAF4b occupies the proximal promoters of Dazl, Stra8, Figlα, and Nobox, ultimately resulting in their expression.Proper expression of these essential regulators facilitates expression of downstream meiosis and oogenesis genes, finally leading to the development of a healthy primordial follicle pool. Red arrows represent direct transcriptional regulation, gray arrows represent post-transcriptional regulation, and dashed lines indicate putative mechanisms.

Mentions: We propose a model (Fig 8) for TAF4b function in which TAF4b occupies the proximal promoters of a subset of essential meiosis- and oogenesis-regulating genes, including Stra8, Figlα, Nobox, and Dazl, distinguishing TAF4b as a novel upstream “regulator of meiotic regulators”. Proper expression of these proteins can then lead to the faithful activation of downstream meiosis and oogenesis genes, including Sycp1/2/3, Msy2, and Figlα and Nobox target genes. Ultimately, we propose that TAF4b is required for a gene regulatory network essential for successful prophase I progression and for the establishment of a healthy primordial follicle reserve.


TAF4b Regulates Oocyte-Specific Genes Essential for Meiosis.

Grive KJ, Gustafson EA, Seymour KA, Baddoo M, Schorl C, Golnoski K, Rajkovic A, Brodsky AS, Freiman RN - PLoS Genet. (2016)

Model of TAF4b promoting a critical meiosis and oogenesis gene regulatory network: Data from chromatin immunoprecipitation experiments demonstrates that TAF4b occupies the proximal promoters of Dazl, Stra8, Figlα, and Nobox, ultimately resulting in their expression.Proper expression of these essential regulators facilitates expression of downstream meiosis and oogenesis genes, finally leading to the development of a healthy primordial follicle pool. Red arrows represent direct transcriptional regulation, gray arrows represent post-transcriptional regulation, and dashed lines indicate putative mechanisms.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4920394&req=5

pgen.1006128.g008: Model of TAF4b promoting a critical meiosis and oogenesis gene regulatory network: Data from chromatin immunoprecipitation experiments demonstrates that TAF4b occupies the proximal promoters of Dazl, Stra8, Figlα, and Nobox, ultimately resulting in their expression.Proper expression of these essential regulators facilitates expression of downstream meiosis and oogenesis genes, finally leading to the development of a healthy primordial follicle pool. Red arrows represent direct transcriptional regulation, gray arrows represent post-transcriptional regulation, and dashed lines indicate putative mechanisms.
Mentions: We propose a model (Fig 8) for TAF4b function in which TAF4b occupies the proximal promoters of a subset of essential meiosis- and oogenesis-regulating genes, including Stra8, Figlα, Nobox, and Dazl, distinguishing TAF4b as a novel upstream “regulator of meiotic regulators”. Proper expression of these proteins can then lead to the faithful activation of downstream meiosis and oogenesis genes, including Sycp1/2/3, Msy2, and Figlα and Nobox target genes. Ultimately, we propose that TAF4b is required for a gene regulatory network essential for successful prophase I progression and for the establishment of a healthy primordial follicle reserve.

Bottom Line: To further explore the potential mechanism of TAF4b in promoting ovarian follicle development, we analyzed global gene expression at multiple time points in the human fetal ovary.This computational analysis revealed coordinate expression of human TAF4B and critical regulators and effectors of meiosis I including SYCP3, YBX2, STAG3, and DAZL.Accordingly, TAF4b occupies the proximal promoters of many essential meiosis and oogenesis regulators, including Stra8, Dazl, Figla, and Nobox, and is required for their proper expression.

View Article: PubMed Central - PubMed

Affiliation: MCB Graduate Program, Brown University, Providence, Rhode Island, United States of America.

ABSTRACT
TAF4b is a gonadal-enriched subunit of the general transcription factor TFIID that is implicated in promoting healthy ovarian aging and female fertility in mice and humans. To further explore the potential mechanism of TAF4b in promoting ovarian follicle development, we analyzed global gene expression at multiple time points in the human fetal ovary. This computational analysis revealed coordinate expression of human TAF4B and critical regulators and effectors of meiosis I including SYCP3, YBX2, STAG3, and DAZL. To address the functional relevance of this analysis, we turned to the embryonic Taf4b-deficient mouse ovary where, for the first time, we demonstrate, severe deficits in prophase I progression as well as asynapsis in Taf4b-deficient oocytes. Accordingly, TAF4b occupies the proximal promoters of many essential meiosis and oogenesis regulators, including Stra8, Dazl, Figla, and Nobox, and is required for their proper expression. These data reveal a novel TAF4b function in regulating a meiotic gene expression program in early mouse oogenesis, and support the existence of a highly conserved TAF4b-dependent gene regulatory network promoting early oocyte development in both mice and women.

No MeSH data available.


Related in: MedlinePlus