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Frequency Responses of Rat Retinal Ganglion Cells.

Hadjinicolaou AE, Cloherty SL, Hung YS, Kameneva T, Ibbotson MR - PLoS ONE (2016)

Bottom Line: The least responsive cells were the B2 and C3 types (peaks: 2-5 Hz, cutoffs: 8-11 Hz).We found no difference between cells stratifying in the inner and outer IPL (i.e., ON and OFF cells) or between cells with large and small somas or dendritic fields.Intrinsic physiological properties (input resistance, spike width and sag) had little impact on frequency response at low frequencies, but account for 30-40% of response variability at frequencies >30 Hz.

View Article: PubMed Central - PubMed

Affiliation: National Vision Research Institute, Australian College of Optometry, Carlton, Victoria, Australia.

ABSTRACT
There are 15-20 different types of retinal ganglion cells (RGC) in the mammalian retina, each encoding different aspects of the visual scene. The mechanism by which post-synaptic signals from the retinal network generate spikes is determined by each cell's intrinsic electrical properties. Here we investigate the frequency responses of morphologically identified rat RGCs using intracellular injection of sinusoidal current waveforms, to assess their intrinsic capabilities with minimal contributions from the retinal network. Recorded cells were classified according to their morphological characteristics (A, B, C or D-type) and their stratification (inner (i), outer (o) or bistratified) in the inner plexiform layer (IPL). Most cell types had low- or band-pass frequency responses. A2, C1 and C4o cells were band-pass with peaks of 15-30 Hz and low-pass cutoffs above 56 Hz (A2 cells) and ~42 Hz (C1 and C4o cells). A1 and C2i/o cells were low-pass with peaks of 10-15 Hz (cutoffs 19-25 Hz). Bistratified D1 and D2 cells were also low-pass with peaks of 5-10 Hz (cutoffs ~16 Hz). The least responsive cells were the B2 and C3 types (peaks: 2-5 Hz, cutoffs: 8-11 Hz). We found no difference between cells stratifying in the inner and outer IPL (i.e., ON and OFF cells) or between cells with large and small somas or dendritic fields. Intrinsic physiological properties (input resistance, spike width and sag) had little impact on frequency response at low frequencies, but account for 30-40% of response variability at frequencies >30 Hz.

No MeSH data available.


Related in: MedlinePlus

Frequency response of retinal ganglion cells.Panels (A–D) show the recorded frequency response of each recorded cell grouped by morphological cell type (A, A-type; B, B-type; C, C-type and D, D-type). Panels (E–H) show the frequency response of each morphological cell type, averaged across all recorded cells of each type. Error bars indicate plus/minus one standard deviation. These data are summarized in panel (J), which shows the 3 dB cut-off frequency for each morphological cell type for which at least two cells were recorded. Panel (I) summarizes the significant differences between morphological cell types at each stimulation frequency (t-tests, p < 0.05). (K) Mean threshold currents for 10 Hz sinusoidal stimulation for each cell type. Error bars indicate plus/minus one standard deviation. The cell counts for each morphological cell type are indicated in parentheses in the figure legends.
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pone.0157676.g003: Frequency response of retinal ganglion cells.Panels (A–D) show the recorded frequency response of each recorded cell grouped by morphological cell type (A, A-type; B, B-type; C, C-type and D, D-type). Panels (E–H) show the frequency response of each morphological cell type, averaged across all recorded cells of each type. Error bars indicate plus/minus one standard deviation. These data are summarized in panel (J), which shows the 3 dB cut-off frequency for each morphological cell type for which at least two cells were recorded. Panel (I) summarizes the significant differences between morphological cell types at each stimulation frequency (t-tests, p < 0.05). (K) Mean threshold currents for 10 Hz sinusoidal stimulation for each cell type. Error bars indicate plus/minus one standard deviation. The cell counts for each morphological cell type are indicated in parentheses in the figure legends.

Mentions: Fig 2 shows patch-clamp recording of responses to intracellular injection of sinusoidal stimulus currents in an A2o-type RGC at 10 Hz (A), 25 Hz (B), and 60 Hz (C). It is evident for this cell that a membrane oscillation occurs for every cycle for all three frequencies. However, only for 10 Hz is a spike also generated by every cycle of the input stimulus. We found significant differences in the frequency spike-responses of different morphological RGC types (Fig 3). The frequency responses of each recorded cell, grouped by morphological cell type, are shown in Fig 3A–3D. For each cell, spiking frequency over the test period was averaged across trials for each stimulus frequency tested. Fig 3E–3H shows the mean frequency response, averaged over all cells, for each morphological cell type. We compared spiking frequency between cell subtypes within each broad morphological cell type (A-, B-, C-, and D-type), with significant differences at each stimulus frequency summarized in Fig 3I. Threshold currents (i.e., the minimum current amplitude required to elicit at least one spike per stimulus cycle for a 10 Hz sinusoid) for each subtype are shown in Fig 3K.


Frequency Responses of Rat Retinal Ganglion Cells.

Hadjinicolaou AE, Cloherty SL, Hung YS, Kameneva T, Ibbotson MR - PLoS ONE (2016)

Frequency response of retinal ganglion cells.Panels (A–D) show the recorded frequency response of each recorded cell grouped by morphological cell type (A, A-type; B, B-type; C, C-type and D, D-type). Panels (E–H) show the frequency response of each morphological cell type, averaged across all recorded cells of each type. Error bars indicate plus/minus one standard deviation. These data are summarized in panel (J), which shows the 3 dB cut-off frequency for each morphological cell type for which at least two cells were recorded. Panel (I) summarizes the significant differences between morphological cell types at each stimulation frequency (t-tests, p < 0.05). (K) Mean threshold currents for 10 Hz sinusoidal stimulation for each cell type. Error bars indicate plus/minus one standard deviation. The cell counts for each morphological cell type are indicated in parentheses in the figure legends.
© Copyright Policy
Related In: Results  -  Collection

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Show All Figures
getmorefigures.php?uid=PMC4920367&req=5

pone.0157676.g003: Frequency response of retinal ganglion cells.Panels (A–D) show the recorded frequency response of each recorded cell grouped by morphological cell type (A, A-type; B, B-type; C, C-type and D, D-type). Panels (E–H) show the frequency response of each morphological cell type, averaged across all recorded cells of each type. Error bars indicate plus/minus one standard deviation. These data are summarized in panel (J), which shows the 3 dB cut-off frequency for each morphological cell type for which at least two cells were recorded. Panel (I) summarizes the significant differences between morphological cell types at each stimulation frequency (t-tests, p < 0.05). (K) Mean threshold currents for 10 Hz sinusoidal stimulation for each cell type. Error bars indicate plus/minus one standard deviation. The cell counts for each morphological cell type are indicated in parentheses in the figure legends.
Mentions: Fig 2 shows patch-clamp recording of responses to intracellular injection of sinusoidal stimulus currents in an A2o-type RGC at 10 Hz (A), 25 Hz (B), and 60 Hz (C). It is evident for this cell that a membrane oscillation occurs for every cycle for all three frequencies. However, only for 10 Hz is a spike also generated by every cycle of the input stimulus. We found significant differences in the frequency spike-responses of different morphological RGC types (Fig 3). The frequency responses of each recorded cell, grouped by morphological cell type, are shown in Fig 3A–3D. For each cell, spiking frequency over the test period was averaged across trials for each stimulus frequency tested. Fig 3E–3H shows the mean frequency response, averaged over all cells, for each morphological cell type. We compared spiking frequency between cell subtypes within each broad morphological cell type (A-, B-, C-, and D-type), with significant differences at each stimulus frequency summarized in Fig 3I. Threshold currents (i.e., the minimum current amplitude required to elicit at least one spike per stimulus cycle for a 10 Hz sinusoid) for each subtype are shown in Fig 3K.

Bottom Line: The least responsive cells were the B2 and C3 types (peaks: 2-5 Hz, cutoffs: 8-11 Hz).We found no difference between cells stratifying in the inner and outer IPL (i.e., ON and OFF cells) or between cells with large and small somas or dendritic fields.Intrinsic physiological properties (input resistance, spike width and sag) had little impact on frequency response at low frequencies, but account for 30-40% of response variability at frequencies >30 Hz.

View Article: PubMed Central - PubMed

Affiliation: National Vision Research Institute, Australian College of Optometry, Carlton, Victoria, Australia.

ABSTRACT
There are 15-20 different types of retinal ganglion cells (RGC) in the mammalian retina, each encoding different aspects of the visual scene. The mechanism by which post-synaptic signals from the retinal network generate spikes is determined by each cell's intrinsic electrical properties. Here we investigate the frequency responses of morphologically identified rat RGCs using intracellular injection of sinusoidal current waveforms, to assess their intrinsic capabilities with minimal contributions from the retinal network. Recorded cells were classified according to their morphological characteristics (A, B, C or D-type) and their stratification (inner (i), outer (o) or bistratified) in the inner plexiform layer (IPL). Most cell types had low- or band-pass frequency responses. A2, C1 and C4o cells were band-pass with peaks of 15-30 Hz and low-pass cutoffs above 56 Hz (A2 cells) and ~42 Hz (C1 and C4o cells). A1 and C2i/o cells were low-pass with peaks of 10-15 Hz (cutoffs 19-25 Hz). Bistratified D1 and D2 cells were also low-pass with peaks of 5-10 Hz (cutoffs ~16 Hz). The least responsive cells were the B2 and C3 types (peaks: 2-5 Hz, cutoffs: 8-11 Hz). We found no difference between cells stratifying in the inner and outer IPL (i.e., ON and OFF cells) or between cells with large and small somas or dendritic fields. Intrinsic physiological properties (input resistance, spike width and sag) had little impact on frequency response at low frequencies, but account for 30-40% of response variability at frequencies >30 Hz.

No MeSH data available.


Related in: MedlinePlus