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Frequency Responses of Rat Retinal Ganglion Cells.

Hadjinicolaou AE, Cloherty SL, Hung YS, Kameneva T, Ibbotson MR - PLoS ONE (2016)

Bottom Line: The least responsive cells were the B2 and C3 types (peaks: 2-5 Hz, cutoffs: 8-11 Hz).We found no difference between cells stratifying in the inner and outer IPL (i.e., ON and OFF cells) or between cells with large and small somas or dendritic fields.Intrinsic physiological properties (input resistance, spike width and sag) had little impact on frequency response at low frequencies, but account for 30-40% of response variability at frequencies >30 Hz.

View Article: PubMed Central - PubMed

Affiliation: National Vision Research Institute, Australian College of Optometry, Carlton, Victoria, Australia.

ABSTRACT
There are 15-20 different types of retinal ganglion cells (RGC) in the mammalian retina, each encoding different aspects of the visual scene. The mechanism by which post-synaptic signals from the retinal network generate spikes is determined by each cell's intrinsic electrical properties. Here we investigate the frequency responses of morphologically identified rat RGCs using intracellular injection of sinusoidal current waveforms, to assess their intrinsic capabilities with minimal contributions from the retinal network. Recorded cells were classified according to their morphological characteristics (A, B, C or D-type) and their stratification (inner (i), outer (o) or bistratified) in the inner plexiform layer (IPL). Most cell types had low- or band-pass frequency responses. A2, C1 and C4o cells were band-pass with peaks of 15-30 Hz and low-pass cutoffs above 56 Hz (A2 cells) and ~42 Hz (C1 and C4o cells). A1 and C2i/o cells were low-pass with peaks of 10-15 Hz (cutoffs 19-25 Hz). Bistratified D1 and D2 cells were also low-pass with peaks of 5-10 Hz (cutoffs ~16 Hz). The least responsive cells were the B2 and C3 types (peaks: 2-5 Hz, cutoffs: 8-11 Hz). We found no difference between cells stratifying in the inner and outer IPL (i.e., ON and OFF cells) or between cells with large and small somas or dendritic fields. Intrinsic physiological properties (input resistance, spike width and sag) had little impact on frequency response at low frequencies, but account for 30-40% of response variability at frequencies >30 Hz.

No MeSH data available.


Related in: MedlinePlus

Reconstruction of recorded cell morphology.Representative confocal image stacks typical of those used for classification of recorded retinal ganglion cells (RGCs) according to their morphological cell type. Examples are shown for representative (A) A-type, (B) B-type, (C) C-type, (D) D-type, (E) ON [C2i], (F) OFF [A2o], and (G) ON-OFF [D2] RGCs. Panels (E–G) show the en face representation (top), revealing the scale and extent of the dendritic arborisation, and a cross-section (bottom), revealing where the dendrites of each cell stratify within the inner plexiform layer (IPL). Long-dashed lines indicate the boundary of the IPL and short-dashed lines indicate the approximate boundary between the two sublaminae. Recorded cells were labelled, via the patch pipette, with Alexa488 (green). Other cells in the ganglion cell layer (GCL) and inner nuclear layer (INL) were labelled with propidium iodide (red).
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pone.0157676.g001: Reconstruction of recorded cell morphology.Representative confocal image stacks typical of those used for classification of recorded retinal ganglion cells (RGCs) according to their morphological cell type. Examples are shown for representative (A) A-type, (B) B-type, (C) C-type, (D) D-type, (E) ON [C2i], (F) OFF [A2o], and (G) ON-OFF [D2] RGCs. Panels (E–G) show the en face representation (top), revealing the scale and extent of the dendritic arborisation, and a cross-section (bottom), revealing where the dendrites of each cell stratify within the inner plexiform layer (IPL). Long-dashed lines indicate the boundary of the IPL and short-dashed lines indicate the approximate boundary between the two sublaminae. Recorded cells were labelled, via the patch pipette, with Alexa488 (green). Other cells in the ganglion cell layer (GCL) and inner nuclear layer (INL) were labelled with propidium iodide (red).

Mentions: Ganglion cells were reconstructed in 3D with a confocal microscope (Zeiss PASCAL) and classified morphologically into types according to well-established criteria [5,7,10]. Reconstructions for 100 of the 150 recorded cells were collected—cells that could not be classified were excluded from further analysis. Generally, cells classified as A-type (A1, A2; n = 43) had large somata and large dendritic fields (Fig 1A and 1F), cells with small-to-medium-sized somata and dendritic fields were classified as B-type (B2, B3, B4; n = 4) (Fig 1B), and cells with small-to-medium-sized somata and medium-to-large dendritic fields were designated as C-type (C1, C2, C3, C4; n = 27) (Fig 1C and 1E). Bistratified cells were classified as D-type RGCs D1, D2; n = 26) (Fig 1D and 1G).


Frequency Responses of Rat Retinal Ganglion Cells.

Hadjinicolaou AE, Cloherty SL, Hung YS, Kameneva T, Ibbotson MR - PLoS ONE (2016)

Reconstruction of recorded cell morphology.Representative confocal image stacks typical of those used for classification of recorded retinal ganglion cells (RGCs) according to their morphological cell type. Examples are shown for representative (A) A-type, (B) B-type, (C) C-type, (D) D-type, (E) ON [C2i], (F) OFF [A2o], and (G) ON-OFF [D2] RGCs. Panels (E–G) show the en face representation (top), revealing the scale and extent of the dendritic arborisation, and a cross-section (bottom), revealing where the dendrites of each cell stratify within the inner plexiform layer (IPL). Long-dashed lines indicate the boundary of the IPL and short-dashed lines indicate the approximate boundary between the two sublaminae. Recorded cells were labelled, via the patch pipette, with Alexa488 (green). Other cells in the ganglion cell layer (GCL) and inner nuclear layer (INL) were labelled with propidium iodide (red).
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4920367&req=5

pone.0157676.g001: Reconstruction of recorded cell morphology.Representative confocal image stacks typical of those used for classification of recorded retinal ganglion cells (RGCs) according to their morphological cell type. Examples are shown for representative (A) A-type, (B) B-type, (C) C-type, (D) D-type, (E) ON [C2i], (F) OFF [A2o], and (G) ON-OFF [D2] RGCs. Panels (E–G) show the en face representation (top), revealing the scale and extent of the dendritic arborisation, and a cross-section (bottom), revealing where the dendrites of each cell stratify within the inner plexiform layer (IPL). Long-dashed lines indicate the boundary of the IPL and short-dashed lines indicate the approximate boundary between the two sublaminae. Recorded cells were labelled, via the patch pipette, with Alexa488 (green). Other cells in the ganglion cell layer (GCL) and inner nuclear layer (INL) were labelled with propidium iodide (red).
Mentions: Ganglion cells were reconstructed in 3D with a confocal microscope (Zeiss PASCAL) and classified morphologically into types according to well-established criteria [5,7,10]. Reconstructions for 100 of the 150 recorded cells were collected—cells that could not be classified were excluded from further analysis. Generally, cells classified as A-type (A1, A2; n = 43) had large somata and large dendritic fields (Fig 1A and 1F), cells with small-to-medium-sized somata and dendritic fields were classified as B-type (B2, B3, B4; n = 4) (Fig 1B), and cells with small-to-medium-sized somata and medium-to-large dendritic fields were designated as C-type (C1, C2, C3, C4; n = 27) (Fig 1C and 1E). Bistratified cells were classified as D-type RGCs D1, D2; n = 26) (Fig 1D and 1G).

Bottom Line: The least responsive cells were the B2 and C3 types (peaks: 2-5 Hz, cutoffs: 8-11 Hz).We found no difference between cells stratifying in the inner and outer IPL (i.e., ON and OFF cells) or between cells with large and small somas or dendritic fields.Intrinsic physiological properties (input resistance, spike width and sag) had little impact on frequency response at low frequencies, but account for 30-40% of response variability at frequencies >30 Hz.

View Article: PubMed Central - PubMed

Affiliation: National Vision Research Institute, Australian College of Optometry, Carlton, Victoria, Australia.

ABSTRACT
There are 15-20 different types of retinal ganglion cells (RGC) in the mammalian retina, each encoding different aspects of the visual scene. The mechanism by which post-synaptic signals from the retinal network generate spikes is determined by each cell's intrinsic electrical properties. Here we investigate the frequency responses of morphologically identified rat RGCs using intracellular injection of sinusoidal current waveforms, to assess their intrinsic capabilities with minimal contributions from the retinal network. Recorded cells were classified according to their morphological characteristics (A, B, C or D-type) and their stratification (inner (i), outer (o) or bistratified) in the inner plexiform layer (IPL). Most cell types had low- or band-pass frequency responses. A2, C1 and C4o cells were band-pass with peaks of 15-30 Hz and low-pass cutoffs above 56 Hz (A2 cells) and ~42 Hz (C1 and C4o cells). A1 and C2i/o cells were low-pass with peaks of 10-15 Hz (cutoffs 19-25 Hz). Bistratified D1 and D2 cells were also low-pass with peaks of 5-10 Hz (cutoffs ~16 Hz). The least responsive cells were the B2 and C3 types (peaks: 2-5 Hz, cutoffs: 8-11 Hz). We found no difference between cells stratifying in the inner and outer IPL (i.e., ON and OFF cells) or between cells with large and small somas or dendritic fields. Intrinsic physiological properties (input resistance, spike width and sag) had little impact on frequency response at low frequencies, but account for 30-40% of response variability at frequencies >30 Hz.

No MeSH data available.


Related in: MedlinePlus