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Fusarium graminearum pyruvate dehydrogenase kinase 1 (FgPDK1) Is Critical for Conidiation, Mycelium Growth, and Pathogenicity.

Gao T, Chen J, Shi Z - PLoS ONE (2016)

Bottom Line: The deletion of FgPDK1 in F. graminearum resulted in the increase in PDH activity, coinciding with several phenotypic defects, such as growth retardation, failure in perithecia and conidia production, and increase in pigment formation.The deletion of FgPDK1 also prohibited the production of deoxynivalenol (DON) and pathogenicity of F. graminearum, which may resulted from the decrease in the expression of Tri6.Taken together, this study firstly identified the vital roles of FgPDK1 in the development of phytopathogen F. graminearum, which may provide a potentially novel clue for target-directed development of agricultural fungicides.

View Article: PubMed Central - PubMed

Affiliation: Institute of Food Quality and Safety, Jiangsu Academy of Agricultural Sciences, Nanjing, China.

ABSTRACT
Pyruvate dehydrogenase kinase (PDK) is an important mitochondrial enzyme that blocks the production of acetyl-CoA by selectively inhibiting the activity of pyruvate dehydrogenase (PDH) through phosphorylation. PDK is an effectively therapeutic target in cancer cells, but the physiological roles of PDK in phytopathogens are largely unknown. To address these gaps, a PDK gene (FgPDK1) was isolated from Fusarium graminearum that is an economically important pathogen infecting cereals. The deletion of FgPDK1 in F. graminearum resulted in the increase in PDH activity, coinciding with several phenotypic defects, such as growth retardation, failure in perithecia and conidia production, and increase in pigment formation. The ΔFgPDK1 mutants showed enhanced sensitivity to osmotic stress and cell membrane-damaging agent. Physiological detection indicated that reactive oxygen species (ROS) accumulation and plasma membrane damage (indicated by PI staining, lipid peroxidation, and electrolyte leakage) occurred in ΔFgPDK1 mutants. The deletion of FgPDK1 also prohibited the production of deoxynivalenol (DON) and pathogenicity of F. graminearum, which may resulted from the decrease in the expression of Tri6. Taken together, this study firstly identified the vital roles of FgPDK1 in the development of phytopathogen F. graminearum, which may provide a potentially novel clue for target-directed development of agricultural fungicides.

No MeSH data available.


Related in: MedlinePlus

Pathogenicity assays for strain PH-1, the FgPDK1 deletion mutant (ΔFgPDK1), and the complemented strain (ΔFgPDK1-C).(A) Symptoms on flowering wheat heads inoculated with water (mock) or with mycelium of strains. Wheat heads were photographed 2 weeks after inoculation. (B) Lesions caused by the infection of different strains as indicated. Lesions were photographed after 5 days at 100% RH at 30°C.
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pone.0158077.g009: Pathogenicity assays for strain PH-1, the FgPDK1 deletion mutant (ΔFgPDK1), and the complemented strain (ΔFgPDK1-C).(A) Symptoms on flowering wheat heads inoculated with water (mock) or with mycelium of strains. Wheat heads were photographed 2 weeks after inoculation. (B) Lesions caused by the infection of different strains as indicated. Lesions were photographed after 5 days at 100% RH at 30°C.

Mentions: Both wheat spikes and tomatoes were selected to test the pathogenicity of ΔFgPDK1 mutants. Fifteen days after flowering wheat heads were point inoculated with mycelial plug, the wild-type and the complemented strain induced scab symptoms on >50% of the inoculated wheat head while ΔFgPDK1 had reduced symptoms only near the point of inoculation (Fig 9A and Table 1). The similar results were also obtained from tomatoes, which wild-type and ΔFgPDK1-C produced serious lesions while ΔFgPDK1 mutants failed to infect tomatoes (Fig 9B). All these results demonstrated that the full virulence of F. graminearum required FgPDK1.


Fusarium graminearum pyruvate dehydrogenase kinase 1 (FgPDK1) Is Critical for Conidiation, Mycelium Growth, and Pathogenicity.

Gao T, Chen J, Shi Z - PLoS ONE (2016)

Pathogenicity assays for strain PH-1, the FgPDK1 deletion mutant (ΔFgPDK1), and the complemented strain (ΔFgPDK1-C).(A) Symptoms on flowering wheat heads inoculated with water (mock) or with mycelium of strains. Wheat heads were photographed 2 weeks after inoculation. (B) Lesions caused by the infection of different strains as indicated. Lesions were photographed after 5 days at 100% RH at 30°C.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4920349&req=5

pone.0158077.g009: Pathogenicity assays for strain PH-1, the FgPDK1 deletion mutant (ΔFgPDK1), and the complemented strain (ΔFgPDK1-C).(A) Symptoms on flowering wheat heads inoculated with water (mock) or with mycelium of strains. Wheat heads were photographed 2 weeks after inoculation. (B) Lesions caused by the infection of different strains as indicated. Lesions were photographed after 5 days at 100% RH at 30°C.
Mentions: Both wheat spikes and tomatoes were selected to test the pathogenicity of ΔFgPDK1 mutants. Fifteen days after flowering wheat heads were point inoculated with mycelial plug, the wild-type and the complemented strain induced scab symptoms on >50% of the inoculated wheat head while ΔFgPDK1 had reduced symptoms only near the point of inoculation (Fig 9A and Table 1). The similar results were also obtained from tomatoes, which wild-type and ΔFgPDK1-C produced serious lesions while ΔFgPDK1 mutants failed to infect tomatoes (Fig 9B). All these results demonstrated that the full virulence of F. graminearum required FgPDK1.

Bottom Line: The deletion of FgPDK1 in F. graminearum resulted in the increase in PDH activity, coinciding with several phenotypic defects, such as growth retardation, failure in perithecia and conidia production, and increase in pigment formation.The deletion of FgPDK1 also prohibited the production of deoxynivalenol (DON) and pathogenicity of F. graminearum, which may resulted from the decrease in the expression of Tri6.Taken together, this study firstly identified the vital roles of FgPDK1 in the development of phytopathogen F. graminearum, which may provide a potentially novel clue for target-directed development of agricultural fungicides.

View Article: PubMed Central - PubMed

Affiliation: Institute of Food Quality and Safety, Jiangsu Academy of Agricultural Sciences, Nanjing, China.

ABSTRACT
Pyruvate dehydrogenase kinase (PDK) is an important mitochondrial enzyme that blocks the production of acetyl-CoA by selectively inhibiting the activity of pyruvate dehydrogenase (PDH) through phosphorylation. PDK is an effectively therapeutic target in cancer cells, but the physiological roles of PDK in phytopathogens are largely unknown. To address these gaps, a PDK gene (FgPDK1) was isolated from Fusarium graminearum that is an economically important pathogen infecting cereals. The deletion of FgPDK1 in F. graminearum resulted in the increase in PDH activity, coinciding with several phenotypic defects, such as growth retardation, failure in perithecia and conidia production, and increase in pigment formation. The ΔFgPDK1 mutants showed enhanced sensitivity to osmotic stress and cell membrane-damaging agent. Physiological detection indicated that reactive oxygen species (ROS) accumulation and plasma membrane damage (indicated by PI staining, lipid peroxidation, and electrolyte leakage) occurred in ΔFgPDK1 mutants. The deletion of FgPDK1 also prohibited the production of deoxynivalenol (DON) and pathogenicity of F. graminearum, which may resulted from the decrease in the expression of Tri6. Taken together, this study firstly identified the vital roles of FgPDK1 in the development of phytopathogen F. graminearum, which may provide a potentially novel clue for target-directed development of agricultural fungicides.

No MeSH data available.


Related in: MedlinePlus