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Fusarium graminearum pyruvate dehydrogenase kinase 1 (FgPDK1) Is Critical for Conidiation, Mycelium Growth, and Pathogenicity.

Gao T, Chen J, Shi Z - PLoS ONE (2016)

Bottom Line: The deletion of FgPDK1 in F. graminearum resulted in the increase in PDH activity, coinciding with several phenotypic defects, such as growth retardation, failure in perithecia and conidia production, and increase in pigment formation.The deletion of FgPDK1 also prohibited the production of deoxynivalenol (DON) and pathogenicity of F. graminearum, which may resulted from the decrease in the expression of Tri6.Taken together, this study firstly identified the vital roles of FgPDK1 in the development of phytopathogen F. graminearum, which may provide a potentially novel clue for target-directed development of agricultural fungicides.

View Article: PubMed Central - PubMed

Affiliation: Institute of Food Quality and Safety, Jiangsu Academy of Agricultural Sciences, Nanjing, China.

ABSTRACT
Pyruvate dehydrogenase kinase (PDK) is an important mitochondrial enzyme that blocks the production of acetyl-CoA by selectively inhibiting the activity of pyruvate dehydrogenase (PDH) through phosphorylation. PDK is an effectively therapeutic target in cancer cells, but the physiological roles of PDK in phytopathogens are largely unknown. To address these gaps, a PDK gene (FgPDK1) was isolated from Fusarium graminearum that is an economically important pathogen infecting cereals. The deletion of FgPDK1 in F. graminearum resulted in the increase in PDH activity, coinciding with several phenotypic defects, such as growth retardation, failure in perithecia and conidia production, and increase in pigment formation. The ΔFgPDK1 mutants showed enhanced sensitivity to osmotic stress and cell membrane-damaging agent. Physiological detection indicated that reactive oxygen species (ROS) accumulation and plasma membrane damage (indicated by PI staining, lipid peroxidation, and electrolyte leakage) occurred in ΔFgPDK1 mutants. The deletion of FgPDK1 also prohibited the production of deoxynivalenol (DON) and pathogenicity of F. graminearum, which may resulted from the decrease in the expression of Tri6. Taken together, this study firstly identified the vital roles of FgPDK1 in the development of phytopathogen F. graminearum, which may provide a potentially novel clue for target-directed development of agricultural fungicides.

No MeSH data available.


Related in: MedlinePlus

Relative conductivity (a) and MDA content (b) of mycelia in PH-1, the FgPDK1 deletion mutant (ΔFgPDK1), and the complemented strain (ΔFgPDK1-C). The mean values of three replicates followed by different letters indicated significance of difference between the treatments (P<0.05, ANOVA, LSD).
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pone.0158077.g007: Relative conductivity (a) and MDA content (b) of mycelia in PH-1, the FgPDK1 deletion mutant (ΔFgPDK1), and the complemented strain (ΔFgPDK1-C). The mean values of three replicates followed by different letters indicated significance of difference between the treatments (P<0.05, ANOVA, LSD).

Mentions: The increase in cell membrane permeability results in the increase in electrolyte leakage, which can be indicated by relative conductivity. In a time-course experiment, the relative conductivities obtained from the distilled water containing mycelia of ΔFgPDK1 mutants were higher than that of wild-type progenitor and the complemented strain (Fig 7A). MDA concentration has been frequently used as an index of lipid peroxidation, indicating the oxidative injury of cell plasma membrane [35]. Compared to wild-type and the complemented strain, the deletion of FgPDK1 led to the significant increase in MDA content in mycelia (Fig 7B). These results suggested that FgPDK1 was important for maintaining cell membrane integrity of F. graminearum.


Fusarium graminearum pyruvate dehydrogenase kinase 1 (FgPDK1) Is Critical for Conidiation, Mycelium Growth, and Pathogenicity.

Gao T, Chen J, Shi Z - PLoS ONE (2016)

Relative conductivity (a) and MDA content (b) of mycelia in PH-1, the FgPDK1 deletion mutant (ΔFgPDK1), and the complemented strain (ΔFgPDK1-C). The mean values of three replicates followed by different letters indicated significance of difference between the treatments (P<0.05, ANOVA, LSD).
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4920349&req=5

pone.0158077.g007: Relative conductivity (a) and MDA content (b) of mycelia in PH-1, the FgPDK1 deletion mutant (ΔFgPDK1), and the complemented strain (ΔFgPDK1-C). The mean values of three replicates followed by different letters indicated significance of difference between the treatments (P<0.05, ANOVA, LSD).
Mentions: The increase in cell membrane permeability results in the increase in electrolyte leakage, which can be indicated by relative conductivity. In a time-course experiment, the relative conductivities obtained from the distilled water containing mycelia of ΔFgPDK1 mutants were higher than that of wild-type progenitor and the complemented strain (Fig 7A). MDA concentration has been frequently used as an index of lipid peroxidation, indicating the oxidative injury of cell plasma membrane [35]. Compared to wild-type and the complemented strain, the deletion of FgPDK1 led to the significant increase in MDA content in mycelia (Fig 7B). These results suggested that FgPDK1 was important for maintaining cell membrane integrity of F. graminearum.

Bottom Line: The deletion of FgPDK1 in F. graminearum resulted in the increase in PDH activity, coinciding with several phenotypic defects, such as growth retardation, failure in perithecia and conidia production, and increase in pigment formation.The deletion of FgPDK1 also prohibited the production of deoxynivalenol (DON) and pathogenicity of F. graminearum, which may resulted from the decrease in the expression of Tri6.Taken together, this study firstly identified the vital roles of FgPDK1 in the development of phytopathogen F. graminearum, which may provide a potentially novel clue for target-directed development of agricultural fungicides.

View Article: PubMed Central - PubMed

Affiliation: Institute of Food Quality and Safety, Jiangsu Academy of Agricultural Sciences, Nanjing, China.

ABSTRACT
Pyruvate dehydrogenase kinase (PDK) is an important mitochondrial enzyme that blocks the production of acetyl-CoA by selectively inhibiting the activity of pyruvate dehydrogenase (PDH) through phosphorylation. PDK is an effectively therapeutic target in cancer cells, but the physiological roles of PDK in phytopathogens are largely unknown. To address these gaps, a PDK gene (FgPDK1) was isolated from Fusarium graminearum that is an economically important pathogen infecting cereals. The deletion of FgPDK1 in F. graminearum resulted in the increase in PDH activity, coinciding with several phenotypic defects, such as growth retardation, failure in perithecia and conidia production, and increase in pigment formation. The ΔFgPDK1 mutants showed enhanced sensitivity to osmotic stress and cell membrane-damaging agent. Physiological detection indicated that reactive oxygen species (ROS) accumulation and plasma membrane damage (indicated by PI staining, lipid peroxidation, and electrolyte leakage) occurred in ΔFgPDK1 mutants. The deletion of FgPDK1 also prohibited the production of deoxynivalenol (DON) and pathogenicity of F. graminearum, which may resulted from the decrease in the expression of Tri6. Taken together, this study firstly identified the vital roles of FgPDK1 in the development of phytopathogen F. graminearum, which may provide a potentially novel clue for target-directed development of agricultural fungicides.

No MeSH data available.


Related in: MedlinePlus