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Fusarium graminearum pyruvate dehydrogenase kinase 1 (FgPDK1) Is Critical for Conidiation, Mycelium Growth, and Pathogenicity.

Gao T, Chen J, Shi Z - PLoS ONE (2016)

Bottom Line: The deletion of FgPDK1 in F. graminearum resulted in the increase in PDH activity, coinciding with several phenotypic defects, such as growth retardation, failure in perithecia and conidia production, and increase in pigment formation.The deletion of FgPDK1 also prohibited the production of deoxynivalenol (DON) and pathogenicity of F. graminearum, which may resulted from the decrease in the expression of Tri6.Taken together, this study firstly identified the vital roles of FgPDK1 in the development of phytopathogen F. graminearum, which may provide a potentially novel clue for target-directed development of agricultural fungicides.

View Article: PubMed Central - PubMed

Affiliation: Institute of Food Quality and Safety, Jiangsu Academy of Agricultural Sciences, Nanjing, China.

ABSTRACT
Pyruvate dehydrogenase kinase (PDK) is an important mitochondrial enzyme that blocks the production of acetyl-CoA by selectively inhibiting the activity of pyruvate dehydrogenase (PDH) through phosphorylation. PDK is an effectively therapeutic target in cancer cells, but the physiological roles of PDK in phytopathogens are largely unknown. To address these gaps, a PDK gene (FgPDK1) was isolated from Fusarium graminearum that is an economically important pathogen infecting cereals. The deletion of FgPDK1 in F. graminearum resulted in the increase in PDH activity, coinciding with several phenotypic defects, such as growth retardation, failure in perithecia and conidia production, and increase in pigment formation. The ΔFgPDK1 mutants showed enhanced sensitivity to osmotic stress and cell membrane-damaging agent. Physiological detection indicated that reactive oxygen species (ROS) accumulation and plasma membrane damage (indicated by PI staining, lipid peroxidation, and electrolyte leakage) occurred in ΔFgPDK1 mutants. The deletion of FgPDK1 also prohibited the production of deoxynivalenol (DON) and pathogenicity of F. graminearum, which may resulted from the decrease in the expression of Tri6. Taken together, this study firstly identified the vital roles of FgPDK1 in the development of phytopathogen F. graminearum, which may provide a potentially novel clue for target-directed development of agricultural fungicides.

No MeSH data available.


Related in: MedlinePlus

The fluorescent staining of ROS in the mycelia of PH-1, the FgPDK1 deletion mutant (ΔFgPDK1), and the complemented strain (ΔFgPDK1-C).Mycelia were incubated with DCFH-DA followed by the fluorescent microscopic observation.
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pone.0158077.g006: The fluorescent staining of ROS in the mycelia of PH-1, the FgPDK1 deletion mutant (ΔFgPDK1), and the complemented strain (ΔFgPDK1-C).Mycelia were incubated with DCFH-DA followed by the fluorescent microscopic observation.

Mentions: To test the cell membrane integrity, we performed PI staining that can only enter membrane-compromised cells to bind nucleic acids [31]. The PI-stained hyphae of ΔFgPDK1 mutants showed more extensive red fluorescence than that of wild-type and the complemented strain (Fig 5). As an important factor for damaging cell membrane, the endogenous ROS detected by specific probe DCFH-DA increased in ΔFgPDK1 mutants than that of wild-type and the complemented strain (Fig 6).


Fusarium graminearum pyruvate dehydrogenase kinase 1 (FgPDK1) Is Critical for Conidiation, Mycelium Growth, and Pathogenicity.

Gao T, Chen J, Shi Z - PLoS ONE (2016)

The fluorescent staining of ROS in the mycelia of PH-1, the FgPDK1 deletion mutant (ΔFgPDK1), and the complemented strain (ΔFgPDK1-C).Mycelia were incubated with DCFH-DA followed by the fluorescent microscopic observation.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4920349&req=5

pone.0158077.g006: The fluorescent staining of ROS in the mycelia of PH-1, the FgPDK1 deletion mutant (ΔFgPDK1), and the complemented strain (ΔFgPDK1-C).Mycelia were incubated with DCFH-DA followed by the fluorescent microscopic observation.
Mentions: To test the cell membrane integrity, we performed PI staining that can only enter membrane-compromised cells to bind nucleic acids [31]. The PI-stained hyphae of ΔFgPDK1 mutants showed more extensive red fluorescence than that of wild-type and the complemented strain (Fig 5). As an important factor for damaging cell membrane, the endogenous ROS detected by specific probe DCFH-DA increased in ΔFgPDK1 mutants than that of wild-type and the complemented strain (Fig 6).

Bottom Line: The deletion of FgPDK1 in F. graminearum resulted in the increase in PDH activity, coinciding with several phenotypic defects, such as growth retardation, failure in perithecia and conidia production, and increase in pigment formation.The deletion of FgPDK1 also prohibited the production of deoxynivalenol (DON) and pathogenicity of F. graminearum, which may resulted from the decrease in the expression of Tri6.Taken together, this study firstly identified the vital roles of FgPDK1 in the development of phytopathogen F. graminearum, which may provide a potentially novel clue for target-directed development of agricultural fungicides.

View Article: PubMed Central - PubMed

Affiliation: Institute of Food Quality and Safety, Jiangsu Academy of Agricultural Sciences, Nanjing, China.

ABSTRACT
Pyruvate dehydrogenase kinase (PDK) is an important mitochondrial enzyme that blocks the production of acetyl-CoA by selectively inhibiting the activity of pyruvate dehydrogenase (PDH) through phosphorylation. PDK is an effectively therapeutic target in cancer cells, but the physiological roles of PDK in phytopathogens are largely unknown. To address these gaps, a PDK gene (FgPDK1) was isolated from Fusarium graminearum that is an economically important pathogen infecting cereals. The deletion of FgPDK1 in F. graminearum resulted in the increase in PDH activity, coinciding with several phenotypic defects, such as growth retardation, failure in perithecia and conidia production, and increase in pigment formation. The ΔFgPDK1 mutants showed enhanced sensitivity to osmotic stress and cell membrane-damaging agent. Physiological detection indicated that reactive oxygen species (ROS) accumulation and plasma membrane damage (indicated by PI staining, lipid peroxidation, and electrolyte leakage) occurred in ΔFgPDK1 mutants. The deletion of FgPDK1 also prohibited the production of deoxynivalenol (DON) and pathogenicity of F. graminearum, which may resulted from the decrease in the expression of Tri6. Taken together, this study firstly identified the vital roles of FgPDK1 in the development of phytopathogen F. graminearum, which may provide a potentially novel clue for target-directed development of agricultural fungicides.

No MeSH data available.


Related in: MedlinePlus