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Fusarium graminearum pyruvate dehydrogenase kinase 1 (FgPDK1) Is Critical for Conidiation, Mycelium Growth, and Pathogenicity.

Gao T, Chen J, Shi Z - PLoS ONE (2016)

Bottom Line: The deletion of FgPDK1 in F. graminearum resulted in the increase in PDH activity, coinciding with several phenotypic defects, such as growth retardation, failure in perithecia and conidia production, and increase in pigment formation.The deletion of FgPDK1 also prohibited the production of deoxynivalenol (DON) and pathogenicity of F. graminearum, which may resulted from the decrease in the expression of Tri6.Taken together, this study firstly identified the vital roles of FgPDK1 in the development of phytopathogen F. graminearum, which may provide a potentially novel clue for target-directed development of agricultural fungicides.

View Article: PubMed Central - PubMed

Affiliation: Institute of Food Quality and Safety, Jiangsu Academy of Agricultural Sciences, Nanjing, China.

ABSTRACT
Pyruvate dehydrogenase kinase (PDK) is an important mitochondrial enzyme that blocks the production of acetyl-CoA by selectively inhibiting the activity of pyruvate dehydrogenase (PDH) through phosphorylation. PDK is an effectively therapeutic target in cancer cells, but the physiological roles of PDK in phytopathogens are largely unknown. To address these gaps, a PDK gene (FgPDK1) was isolated from Fusarium graminearum that is an economically important pathogen infecting cereals. The deletion of FgPDK1 in F. graminearum resulted in the increase in PDH activity, coinciding with several phenotypic defects, such as growth retardation, failure in perithecia and conidia production, and increase in pigment formation. The ΔFgPDK1 mutants showed enhanced sensitivity to osmotic stress and cell membrane-damaging agent. Physiological detection indicated that reactive oxygen species (ROS) accumulation and plasma membrane damage (indicated by PI staining, lipid peroxidation, and electrolyte leakage) occurred in ΔFgPDK1 mutants. The deletion of FgPDK1 also prohibited the production of deoxynivalenol (DON) and pathogenicity of F. graminearum, which may resulted from the decrease in the expression of Tri6. Taken together, this study firstly identified the vital roles of FgPDK1 in the development of phytopathogen F. graminearum, which may provide a potentially novel clue for target-directed development of agricultural fungicides.

No MeSH data available.


Related in: MedlinePlus

Stress response of the wild-type strain PH-1, the FgPDK1 deletion mutant (ΔFgPDK1), and the complemented strain (ΔFgPDK1-C).The strains were grown in medium containing different chemicals as indicated. Then the photographs were taken (A), and the inhibition of mycelial growth were measured (B). The mean values of three replicates followed by different letters indicated significance of difference between the treatments (P<0.05, ANOVA, LSD).
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pone.0158077.g004: Stress response of the wild-type strain PH-1, the FgPDK1 deletion mutant (ΔFgPDK1), and the complemented strain (ΔFgPDK1-C).The strains were grown in medium containing different chemicals as indicated. Then the photographs were taken (A), and the inhibition of mycelial growth were measured (B). The mean values of three replicates followed by different letters indicated significance of difference between the treatments (P<0.05, ANOVA, LSD).

Mentions: In order to ascertain the possible role of FgPDK1 in stress responses of F. graminearum, the ΔFgPDK1 mutants were exposed to several stress-induced reagents (Fig 4). The growth of ΔFgPDK1 mutants was inhibited pronouncedly compared to wild-type strain and the complemented strain under the exposure of NaCl and KCl (Fig 4A), suggesting that the deletion of FgPDK1 increased the sensitivity of F. graminearum to osmotic stress. Treatment with SDS, an inducer of cell membrane damage, resulted in the significant increase in the inhibition rate of mycelial growth of ΔFgPDK1 mutants than that of wild-type and the complemented strain (Fig 4B). However, there were no remarkable changes in mycelial growth among all the tested strains under the exposure of Congo red that can destroy cell wall integrity (Fig 4B). These results suggested that FgPDK1 may be an important regulator of cell membrane in stress responses of F. graminearum.


Fusarium graminearum pyruvate dehydrogenase kinase 1 (FgPDK1) Is Critical for Conidiation, Mycelium Growth, and Pathogenicity.

Gao T, Chen J, Shi Z - PLoS ONE (2016)

Stress response of the wild-type strain PH-1, the FgPDK1 deletion mutant (ΔFgPDK1), and the complemented strain (ΔFgPDK1-C).The strains were grown in medium containing different chemicals as indicated. Then the photographs were taken (A), and the inhibition of mycelial growth were measured (B). The mean values of three replicates followed by different letters indicated significance of difference between the treatments (P<0.05, ANOVA, LSD).
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4920349&req=5

pone.0158077.g004: Stress response of the wild-type strain PH-1, the FgPDK1 deletion mutant (ΔFgPDK1), and the complemented strain (ΔFgPDK1-C).The strains were grown in medium containing different chemicals as indicated. Then the photographs were taken (A), and the inhibition of mycelial growth were measured (B). The mean values of three replicates followed by different letters indicated significance of difference between the treatments (P<0.05, ANOVA, LSD).
Mentions: In order to ascertain the possible role of FgPDK1 in stress responses of F. graminearum, the ΔFgPDK1 mutants were exposed to several stress-induced reagents (Fig 4). The growth of ΔFgPDK1 mutants was inhibited pronouncedly compared to wild-type strain and the complemented strain under the exposure of NaCl and KCl (Fig 4A), suggesting that the deletion of FgPDK1 increased the sensitivity of F. graminearum to osmotic stress. Treatment with SDS, an inducer of cell membrane damage, resulted in the significant increase in the inhibition rate of mycelial growth of ΔFgPDK1 mutants than that of wild-type and the complemented strain (Fig 4B). However, there were no remarkable changes in mycelial growth among all the tested strains under the exposure of Congo red that can destroy cell wall integrity (Fig 4B). These results suggested that FgPDK1 may be an important regulator of cell membrane in stress responses of F. graminearum.

Bottom Line: The deletion of FgPDK1 in F. graminearum resulted in the increase in PDH activity, coinciding with several phenotypic defects, such as growth retardation, failure in perithecia and conidia production, and increase in pigment formation.The deletion of FgPDK1 also prohibited the production of deoxynivalenol (DON) and pathogenicity of F. graminearum, which may resulted from the decrease in the expression of Tri6.Taken together, this study firstly identified the vital roles of FgPDK1 in the development of phytopathogen F. graminearum, which may provide a potentially novel clue for target-directed development of agricultural fungicides.

View Article: PubMed Central - PubMed

Affiliation: Institute of Food Quality and Safety, Jiangsu Academy of Agricultural Sciences, Nanjing, China.

ABSTRACT
Pyruvate dehydrogenase kinase (PDK) is an important mitochondrial enzyme that blocks the production of acetyl-CoA by selectively inhibiting the activity of pyruvate dehydrogenase (PDH) through phosphorylation. PDK is an effectively therapeutic target in cancer cells, but the physiological roles of PDK in phytopathogens are largely unknown. To address these gaps, a PDK gene (FgPDK1) was isolated from Fusarium graminearum that is an economically important pathogen infecting cereals. The deletion of FgPDK1 in F. graminearum resulted in the increase in PDH activity, coinciding with several phenotypic defects, such as growth retardation, failure in perithecia and conidia production, and increase in pigment formation. The ΔFgPDK1 mutants showed enhanced sensitivity to osmotic stress and cell membrane-damaging agent. Physiological detection indicated that reactive oxygen species (ROS) accumulation and plasma membrane damage (indicated by PI staining, lipid peroxidation, and electrolyte leakage) occurred in ΔFgPDK1 mutants. The deletion of FgPDK1 also prohibited the production of deoxynivalenol (DON) and pathogenicity of F. graminearum, which may resulted from the decrease in the expression of Tri6. Taken together, this study firstly identified the vital roles of FgPDK1 in the development of phytopathogen F. graminearum, which may provide a potentially novel clue for target-directed development of agricultural fungicides.

No MeSH data available.


Related in: MedlinePlus