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Fusarium graminearum pyruvate dehydrogenase kinase 1 (FgPDK1) Is Critical for Conidiation, Mycelium Growth, and Pathogenicity.

Gao T, Chen J, Shi Z - PLoS ONE (2016)

Bottom Line: The deletion of FgPDK1 in F. graminearum resulted in the increase in PDH activity, coinciding with several phenotypic defects, such as growth retardation, failure in perithecia and conidia production, and increase in pigment formation.The deletion of FgPDK1 also prohibited the production of deoxynivalenol (DON) and pathogenicity of F. graminearum, which may resulted from the decrease in the expression of Tri6.Taken together, this study firstly identified the vital roles of FgPDK1 in the development of phytopathogen F. graminearum, which may provide a potentially novel clue for target-directed development of agricultural fungicides.

View Article: PubMed Central - PubMed

Affiliation: Institute of Food Quality and Safety, Jiangsu Academy of Agricultural Sciences, Nanjing, China.

ABSTRACT
Pyruvate dehydrogenase kinase (PDK) is an important mitochondrial enzyme that blocks the production of acetyl-CoA by selectively inhibiting the activity of pyruvate dehydrogenase (PDH) through phosphorylation. PDK is an effectively therapeutic target in cancer cells, but the physiological roles of PDK in phytopathogens are largely unknown. To address these gaps, a PDK gene (FgPDK1) was isolated from Fusarium graminearum that is an economically important pathogen infecting cereals. The deletion of FgPDK1 in F. graminearum resulted in the increase in PDH activity, coinciding with several phenotypic defects, such as growth retardation, failure in perithecia and conidia production, and increase in pigment formation. The ΔFgPDK1 mutants showed enhanced sensitivity to osmotic stress and cell membrane-damaging agent. Physiological detection indicated that reactive oxygen species (ROS) accumulation and plasma membrane damage (indicated by PI staining, lipid peroxidation, and electrolyte leakage) occurred in ΔFgPDK1 mutants. The deletion of FgPDK1 also prohibited the production of deoxynivalenol (DON) and pathogenicity of F. graminearum, which may resulted from the decrease in the expression of Tri6. Taken together, this study firstly identified the vital roles of FgPDK1 in the development of phytopathogen F. graminearum, which may provide a potentially novel clue for target-directed development of agricultural fungicides.

No MeSH data available.


Related in: MedlinePlus

Effect of FgPDK1 deletion on PDH activity in F. graminearum.The PDH activity was determined in mycelia of wild-type strain PH-1, the FgPDK1 deletion mutant (ΔFgPDK1), and the complemented strain (ΔFgPDK1-C), respectively. The mean values of three replicates followed by different letters indicated significance of difference between the treatments (P<0.05, ANOVA, LSD).
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pone.0158077.g002: Effect of FgPDK1 deletion on PDH activity in F. graminearum.The PDH activity was determined in mycelia of wild-type strain PH-1, the FgPDK1 deletion mutant (ΔFgPDK1), and the complemented strain (ΔFgPDK1-C), respectively. The mean values of three replicates followed by different letters indicated significance of difference between the treatments (P<0.05, ANOVA, LSD).

Mentions: To address the question of whether the FgPDK1 is an essential prerequisite for the regulation of mitochondrial PDH activity, the mitochondria of the mutant strains were isolated and tested for PDH activity by monitoring the production of NADH in the presence of pyruvate as a substrate. As expect, the deletion of FgPDK1 resulted in the significant increase in the activity of mitochondrial PDH compared with wild-type and the complemented strain (Fig 2).


Fusarium graminearum pyruvate dehydrogenase kinase 1 (FgPDK1) Is Critical for Conidiation, Mycelium Growth, and Pathogenicity.

Gao T, Chen J, Shi Z - PLoS ONE (2016)

Effect of FgPDK1 deletion on PDH activity in F. graminearum.The PDH activity was determined in mycelia of wild-type strain PH-1, the FgPDK1 deletion mutant (ΔFgPDK1), and the complemented strain (ΔFgPDK1-C), respectively. The mean values of three replicates followed by different letters indicated significance of difference between the treatments (P<0.05, ANOVA, LSD).
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4920349&req=5

pone.0158077.g002: Effect of FgPDK1 deletion on PDH activity in F. graminearum.The PDH activity was determined in mycelia of wild-type strain PH-1, the FgPDK1 deletion mutant (ΔFgPDK1), and the complemented strain (ΔFgPDK1-C), respectively. The mean values of three replicates followed by different letters indicated significance of difference between the treatments (P<0.05, ANOVA, LSD).
Mentions: To address the question of whether the FgPDK1 is an essential prerequisite for the regulation of mitochondrial PDH activity, the mitochondria of the mutant strains were isolated and tested for PDH activity by monitoring the production of NADH in the presence of pyruvate as a substrate. As expect, the deletion of FgPDK1 resulted in the significant increase in the activity of mitochondrial PDH compared with wild-type and the complemented strain (Fig 2).

Bottom Line: The deletion of FgPDK1 in F. graminearum resulted in the increase in PDH activity, coinciding with several phenotypic defects, such as growth retardation, failure in perithecia and conidia production, and increase in pigment formation.The deletion of FgPDK1 also prohibited the production of deoxynivalenol (DON) and pathogenicity of F. graminearum, which may resulted from the decrease in the expression of Tri6.Taken together, this study firstly identified the vital roles of FgPDK1 in the development of phytopathogen F. graminearum, which may provide a potentially novel clue for target-directed development of agricultural fungicides.

View Article: PubMed Central - PubMed

Affiliation: Institute of Food Quality and Safety, Jiangsu Academy of Agricultural Sciences, Nanjing, China.

ABSTRACT
Pyruvate dehydrogenase kinase (PDK) is an important mitochondrial enzyme that blocks the production of acetyl-CoA by selectively inhibiting the activity of pyruvate dehydrogenase (PDH) through phosphorylation. PDK is an effectively therapeutic target in cancer cells, but the physiological roles of PDK in phytopathogens are largely unknown. To address these gaps, a PDK gene (FgPDK1) was isolated from Fusarium graminearum that is an economically important pathogen infecting cereals. The deletion of FgPDK1 in F. graminearum resulted in the increase in PDH activity, coinciding with several phenotypic defects, such as growth retardation, failure in perithecia and conidia production, and increase in pigment formation. The ΔFgPDK1 mutants showed enhanced sensitivity to osmotic stress and cell membrane-damaging agent. Physiological detection indicated that reactive oxygen species (ROS) accumulation and plasma membrane damage (indicated by PI staining, lipid peroxidation, and electrolyte leakage) occurred in ΔFgPDK1 mutants. The deletion of FgPDK1 also prohibited the production of deoxynivalenol (DON) and pathogenicity of F. graminearum, which may resulted from the decrease in the expression of Tri6. Taken together, this study firstly identified the vital roles of FgPDK1 in the development of phytopathogen F. graminearum, which may provide a potentially novel clue for target-directed development of agricultural fungicides.

No MeSH data available.


Related in: MedlinePlus