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A phenomenological description of BslA assemblies across multiple length scales.

Morris RJ, Bromley KM, Stanley-Wall N, MacPhee CE - Philos Trans A Math Phys Eng Sci (2016)

Bottom Line: Here we describe several self-assembled structures formed by BslA, both at interfaces and in bulk solution, over a range of length scales spanning from nanometres to millimetres.First, we observe transiently stable and highly elongated air bubbles formed in agitated BslA samples.Second, we describe elongated tubules formed by BslA interfacial films when shear stresses are applied in both a Langmuir trough and a rheometer.

View Article: PubMed Central - PubMed

Affiliation: School of Physics and Astronomy, University of Edinburgh, James Clerk Maxwell Building, Peter Guthrie Tait Road, Edinburgh EH9 3FD, UK.

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(a) SEM image of a tubule obtained from a glutaraldehyde cross-linked sample lifted from a Langmuir trough. The quantity of BslA initially applied to the trough was 254 μg. The tubule appears to be a rolled piece of film with a hollow interior. Scale bar, 50 μm. (b,c) Examples of tubular structures formed under shear in a cone and plate rheometer. An initial concentration of 0.8 mg ml−1 was used for these experiments. Scale bar, 100 μm.
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RSTA20150131F8: (a) SEM image of a tubule obtained from a glutaraldehyde cross-linked sample lifted from a Langmuir trough. The quantity of BslA initially applied to the trough was 254 μg. The tubule appears to be a rolled piece of film with a hollow interior. Scale bar, 50 μm. (b,c) Examples of tubular structures formed under shear in a cone and plate rheometer. An initial concentration of 0.8 mg ml−1 was used for these experiments. Scale bar, 100 μm.

Mentions: To further image the tubule structures, we chemically cross-linked the film using glutaraldehyde. The cross-linked structures were then lifted onto an SEM stub for imaging. Figure 8a shows a tubule imaged using the SEM. From this image, it appears that the tubule consists of a rolled and hollow piece of film.Figure 8.


A phenomenological description of BslA assemblies across multiple length scales.

Morris RJ, Bromley KM, Stanley-Wall N, MacPhee CE - Philos Trans A Math Phys Eng Sci (2016)

(a) SEM image of a tubule obtained from a glutaraldehyde cross-linked sample lifted from a Langmuir trough. The quantity of BslA initially applied to the trough was 254 μg. The tubule appears to be a rolled piece of film with a hollow interior. Scale bar, 50 μm. (b,c) Examples of tubular structures formed under shear in a cone and plate rheometer. An initial concentration of 0.8 mg ml−1 was used for these experiments. Scale bar, 100 μm.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4920280&req=5

RSTA20150131F8: (a) SEM image of a tubule obtained from a glutaraldehyde cross-linked sample lifted from a Langmuir trough. The quantity of BslA initially applied to the trough was 254 μg. The tubule appears to be a rolled piece of film with a hollow interior. Scale bar, 50 μm. (b,c) Examples of tubular structures formed under shear in a cone and plate rheometer. An initial concentration of 0.8 mg ml−1 was used for these experiments. Scale bar, 100 μm.
Mentions: To further image the tubule structures, we chemically cross-linked the film using glutaraldehyde. The cross-linked structures were then lifted onto an SEM stub for imaging. Figure 8a shows a tubule imaged using the SEM. From this image, it appears that the tubule consists of a rolled and hollow piece of film.Figure 8.

Bottom Line: Here we describe several self-assembled structures formed by BslA, both at interfaces and in bulk solution, over a range of length scales spanning from nanometres to millimetres.First, we observe transiently stable and highly elongated air bubbles formed in agitated BslA samples.Second, we describe elongated tubules formed by BslA interfacial films when shear stresses are applied in both a Langmuir trough and a rheometer.

View Article: PubMed Central - PubMed

Affiliation: School of Physics and Astronomy, University of Edinburgh, James Clerk Maxwell Building, Peter Guthrie Tait Road, Edinburgh EH9 3FD, UK.

Show MeSH
Related in: MedlinePlus