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Brief Report: Proatherogenic Cytokine Microenvironment in the Aortic Adventitia of Patients With Rheumatoid Arthritis.

Ahmed A, Hollan I, Curran SA, Kitson SM, Riggio MP, Mikkelsen K, Almdahl SM, Aukrust P, McInnes IB, Goodyear CS - (2016)

Bottom Line: In RA patients, IL-33 expression in endothelial cells correlated positively with the number of swollen joints, suggesting a link between the systemic disease state and the local vascular tissue microlesion.The presence of the proinflammatory cytokines IL-18, IL-33, and TNF may play a role in the inflammatory process within the adventitia that contributes to plaque formation and destabilization.In theory, the amplified expression of these cytokines may contribute to the known increased occurrence and severity of CAD in patients with RA.

View Article: PubMed Central - PubMed

Affiliation: University of Glasgow, Glasgow, UK.

No MeSH data available.


Related in: MedlinePlus

Macrophage, interleukin‐18 (IL‐18), and tumor necrosis factor (TNF) evaluation in the aortic adventitia of late‐stage cardiovascular disease (CVD) patients with (n = 19) and those without (n = 20) rheumatoid arthritis (RA). CD68+ macrophages (A), IL‐18 (B), and TNF (C) were evaluated immunohistologically, and the results were scored as described in Patients and Methods. Data are shown as box plots. Each box represents the 25th to 75th percentiles. Lines inside the boxes represent the median. Lines outside the boxes represent the 5th and 95th percentiles. ∗ = P < 0.05.
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art39574-fig-0001: Macrophage, interleukin‐18 (IL‐18), and tumor necrosis factor (TNF) evaluation in the aortic adventitia of late‐stage cardiovascular disease (CVD) patients with (n = 19) and those without (n = 20) rheumatoid arthritis (RA). CD68+ macrophages (A), IL‐18 (B), and TNF (C) were evaluated immunohistologically, and the results were scored as described in Patients and Methods. Data are shown as box plots. Each box represents the 25th to 75th percentiles. Lines inside the boxes represent the median. Lines outside the boxes represent the 5th and 95th percentiles. ∗ = P < 0.05.

Mentions: The proportion of aortic adventitia biopsy specimens in which CD68+ macrophages were detectable (63% of RA specimens and 56% of non‐RA specimens) and the number of CD68+ cells per section (Figure 1A) were similar in CVD patients with RA and those without RA (additional information is available upon request from the corresponding author). TNF was expressed in 63% of biopsy specimens from RA patients compared with 30% of specimens from non‐RA patients (P = 0.04). In contrast, IL‐18 was present in all biopsy specimens, suggesting ubiquitous expression in the aortic vascular tissue lesion (additional information is available upon request from the corresponding author). Crucially, although no difference in the number of macrophages between RA and non‐RA patients was observed (Figure 1A), the absolute level of IL‐18 and TNF expression in aortic adventitia was higher in RA patients (Figures 1B and C) compared with non‐RA controls (P = 0.03 and P = 0.02, respectively).


Brief Report: Proatherogenic Cytokine Microenvironment in the Aortic Adventitia of Patients With Rheumatoid Arthritis.

Ahmed A, Hollan I, Curran SA, Kitson SM, Riggio MP, Mikkelsen K, Almdahl SM, Aukrust P, McInnes IB, Goodyear CS - (2016)

Macrophage, interleukin‐18 (IL‐18), and tumor necrosis factor (TNF) evaluation in the aortic adventitia of late‐stage cardiovascular disease (CVD) patients with (n = 19) and those without (n = 20) rheumatoid arthritis (RA). CD68+ macrophages (A), IL‐18 (B), and TNF (C) were evaluated immunohistologically, and the results were scored as described in Patients and Methods. Data are shown as box plots. Each box represents the 25th to 75th percentiles. Lines inside the boxes represent the median. Lines outside the boxes represent the 5th and 95th percentiles. ∗ = P < 0.05.
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Related In: Results  -  Collection

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getmorefigures.php?uid=PMC4920270&req=5

art39574-fig-0001: Macrophage, interleukin‐18 (IL‐18), and tumor necrosis factor (TNF) evaluation in the aortic adventitia of late‐stage cardiovascular disease (CVD) patients with (n = 19) and those without (n = 20) rheumatoid arthritis (RA). CD68+ macrophages (A), IL‐18 (B), and TNF (C) were evaluated immunohistologically, and the results were scored as described in Patients and Methods. Data are shown as box plots. Each box represents the 25th to 75th percentiles. Lines inside the boxes represent the median. Lines outside the boxes represent the 5th and 95th percentiles. ∗ = P < 0.05.
Mentions: The proportion of aortic adventitia biopsy specimens in which CD68+ macrophages were detectable (63% of RA specimens and 56% of non‐RA specimens) and the number of CD68+ cells per section (Figure 1A) were similar in CVD patients with RA and those without RA (additional information is available upon request from the corresponding author). TNF was expressed in 63% of biopsy specimens from RA patients compared with 30% of specimens from non‐RA patients (P = 0.04). In contrast, IL‐18 was present in all biopsy specimens, suggesting ubiquitous expression in the aortic vascular tissue lesion (additional information is available upon request from the corresponding author). Crucially, although no difference in the number of macrophages between RA and non‐RA patients was observed (Figure 1A), the absolute level of IL‐18 and TNF expression in aortic adventitia was higher in RA patients (Figures 1B and C) compared with non‐RA controls (P = 0.03 and P = 0.02, respectively).

Bottom Line: In RA patients, IL-33 expression in endothelial cells correlated positively with the number of swollen joints, suggesting a link between the systemic disease state and the local vascular tissue microlesion.The presence of the proinflammatory cytokines IL-18, IL-33, and TNF may play a role in the inflammatory process within the adventitia that contributes to plaque formation and destabilization.In theory, the amplified expression of these cytokines may contribute to the known increased occurrence and severity of CAD in patients with RA.

View Article: PubMed Central - PubMed

Affiliation: University of Glasgow, Glasgow, UK.

No MeSH data available.


Related in: MedlinePlus