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MicroRNA-190 regulates FOXP2 genes in human gastric cancer.

Jia WZ, Yu T, An Q, Yang H, Zhang Z, Liu X, Xiao G - Onco Targets Ther (2016)

Bottom Line: FOXP2-3'-untranslated region (UTR) in miR-190 transfection group was significantly decreased as compared with other groups.There were no significant differences in fluorescence signals of FOXP2mut-3'-UTR in each group.Western blotting results showed the expression level of FOXP2 was significantly lower in GC cell line SGC7901 than in GES-1 cells.

View Article: PubMed Central - PubMed

Affiliation: Department of General Surgery.

ABSTRACT

Objective: To investigate how microRNA-190 (miR-190) regulates FOXP2 genes in gastric cancer (GC) cell line SGC7901.

Methods: We identified that miR-190 could target FOXP2 genes by using dual luciferase enzyme assay. Precursor fragment transfection of miR-190 was performed with GC cell line SGC7901 and human gastric mucosal cell line GES-1. miR-190 expression was detected by reverse transcription-polymerase chain reaction (RT-PCR) and FOXP2 protein expression was measured by Western blotting.

Results: FOXP2-3'-untranslated region (UTR) in miR-190 transfection group was significantly decreased as compared with other groups. There were no significant differences in fluorescence signals of FOXP2mut-3'-UTR in each group. Therefore, it was assumed that miR-190 can target FOXP2 genes. Through RT-PCR verification, it was observed that the expression level of miR-190 was significantly higher in GC cell line SGC7901 than in human gastric mucosa cell line GES-1 after transfection with miR-190 mimics. The expression level of miR-190 was significantly higher in GES-1 cells than in SGC7901 cells after transfection with miR-190 inhibitors. Western blotting results showed the expression level of FOXP2 was significantly lower in GC cell line SGC7901 than in GES-1 cells. Compared with blank, mimics control, and inhibitors control groups, the miR-190 mimics group showed significantly enhanced proliferation, migration, and invasion abilities, while miR-190 inhibitors group showed decreased abilities toward proliferation, migration, and invasion (P<0.05). The transcription level of miR-190 and the expression level of FOXP2 in tumor tissues and adjacent normal tissues in GC patients were verified to be consistent with those of cell line experiments.

Conclusion: Upregulation of miR-190 can lead to downregulation of FOXP2 protein expression. miR-190 may serve as a potential target for GC diagnosis.

No MeSH data available.


Related in: MedlinePlus

Expression of FOXP2 protein detected by Western blotting.Notes: (A) FOXP2 protein expression in SGC7901 cells; (B) FOXP2 protein expression in SGC7901 cells; 1, blank; 2, miR-190 mimics; 3, miR-190 inhibitors; 4, mimics control; 5, inhibitors control.Abbreviation: miR-190, microRNA-190.
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f4-ott-9-3643: Expression of FOXP2 protein detected by Western blotting.Notes: (A) FOXP2 protein expression in SGC7901 cells; (B) FOXP2 protein expression in SGC7901 cells; 1, blank; 2, miR-190 mimics; 3, miR-190 inhibitors; 4, mimics control; 5, inhibitors control.Abbreviation: miR-190, microRNA-190.

Mentions: The results of Western blotting showed that there were no significant differences when mimics control group, inhibitors control group, and blank control group were compared in pairs (all P>0.05) (Table 2, Figures 3 and 4). The expression of FOXP2 significantly decreased (P<0.05) in the miR-190 mimics group when compared with the blank control group.


MicroRNA-190 regulates FOXP2 genes in human gastric cancer.

Jia WZ, Yu T, An Q, Yang H, Zhang Z, Liu X, Xiao G - Onco Targets Ther (2016)

Expression of FOXP2 protein detected by Western blotting.Notes: (A) FOXP2 protein expression in SGC7901 cells; (B) FOXP2 protein expression in SGC7901 cells; 1, blank; 2, miR-190 mimics; 3, miR-190 inhibitors; 4, mimics control; 5, inhibitors control.Abbreviation: miR-190, microRNA-190.
© Copyright Policy
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4920243&req=5

f4-ott-9-3643: Expression of FOXP2 protein detected by Western blotting.Notes: (A) FOXP2 protein expression in SGC7901 cells; (B) FOXP2 protein expression in SGC7901 cells; 1, blank; 2, miR-190 mimics; 3, miR-190 inhibitors; 4, mimics control; 5, inhibitors control.Abbreviation: miR-190, microRNA-190.
Mentions: The results of Western blotting showed that there were no significant differences when mimics control group, inhibitors control group, and blank control group were compared in pairs (all P>0.05) (Table 2, Figures 3 and 4). The expression of FOXP2 significantly decreased (P<0.05) in the miR-190 mimics group when compared with the blank control group.

Bottom Line: FOXP2-3'-untranslated region (UTR) in miR-190 transfection group was significantly decreased as compared with other groups.There were no significant differences in fluorescence signals of FOXP2mut-3'-UTR in each group.Western blotting results showed the expression level of FOXP2 was significantly lower in GC cell line SGC7901 than in GES-1 cells.

View Article: PubMed Central - PubMed

Affiliation: Department of General Surgery.

ABSTRACT

Objective: To investigate how microRNA-190 (miR-190) regulates FOXP2 genes in gastric cancer (GC) cell line SGC7901.

Methods: We identified that miR-190 could target FOXP2 genes by using dual luciferase enzyme assay. Precursor fragment transfection of miR-190 was performed with GC cell line SGC7901 and human gastric mucosal cell line GES-1. miR-190 expression was detected by reverse transcription-polymerase chain reaction (RT-PCR) and FOXP2 protein expression was measured by Western blotting.

Results: FOXP2-3'-untranslated region (UTR) in miR-190 transfection group was significantly decreased as compared with other groups. There were no significant differences in fluorescence signals of FOXP2mut-3'-UTR in each group. Therefore, it was assumed that miR-190 can target FOXP2 genes. Through RT-PCR verification, it was observed that the expression level of miR-190 was significantly higher in GC cell line SGC7901 than in human gastric mucosa cell line GES-1 after transfection with miR-190 mimics. The expression level of miR-190 was significantly higher in GES-1 cells than in SGC7901 cells after transfection with miR-190 inhibitors. Western blotting results showed the expression level of FOXP2 was significantly lower in GC cell line SGC7901 than in GES-1 cells. Compared with blank, mimics control, and inhibitors control groups, the miR-190 mimics group showed significantly enhanced proliferation, migration, and invasion abilities, while miR-190 inhibitors group showed decreased abilities toward proliferation, migration, and invasion (P<0.05). The transcription level of miR-190 and the expression level of FOXP2 in tumor tissues and adjacent normal tissues in GC patients were verified to be consistent with those of cell line experiments.

Conclusion: Upregulation of miR-190 can lead to downregulation of FOXP2 protein expression. miR-190 may serve as a potential target for GC diagnosis.

No MeSH data available.


Related in: MedlinePlus