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Desiccation tolerance in Anopheles coluzzii: the effects of spiracle size and cuticular hydrocarbons.

Arcaz AC, Huestis DL, Dao A, Yaro AS, Diallo M, Andersen J, Blomquist GJ, Lehmann T - J. Exp. Biol. (2016)

Bottom Line: A smaller spiracle size appeared to increase A. coluzzii's desiccation tolerance, but was not statistically significant.Seasonal changes in CHC composition were detected in Sahelian A. coluzzii Stepwise regression models suggested the effect of particular CHCs on desiccation tolerance.In conclusion, the combination of particular CHCs along with the total amount of CHCs is a primary mechanism conferring desiccation tolerance in A. coluzzii, while variation in spiracle size might be a secondary mechanism.

View Article: PubMed Central - PubMed

Affiliation: Laboratory of Malaria and Vector Research, NIAID, NIH, Rockville, MD 20852, USA.

No MeSH data available.


Related in: MedlinePlus

Seasonal difference in DT in wild gravid Anophelescoluzzii and variation in body size in relation to DT. (A) DT in the rainy season (RS) and dry season (DS). Black and blue distributions represent, respectively, the full number and a subset of specimens used in the desiccation assay and in the morphometric and CHC analyses. The seasonal difference is highly significant over the total data (P<0.0003, N=81, Wilcoxon test one-way) and significant in the subset (P<0.047, N=34, Wilcoxon test one-way). (B) Seasonal variation in body size (measured as WL) is summarized in the horizontal box whisker (top; see Results). Red and blue dots represent DT values of gravid mosquitoes from the DS and RS, respectively. The regression line shows the relationship of DT and WL regardless of season (see Results).
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JEB135665F7: Seasonal difference in DT in wild gravid Anophelescoluzzii and variation in body size in relation to DT. (A) DT in the rainy season (RS) and dry season (DS). Black and blue distributions represent, respectively, the full number and a subset of specimens used in the desiccation assay and in the morphometric and CHC analyses. The seasonal difference is highly significant over the total data (P<0.0003, N=81, Wilcoxon test one-way) and significant in the subset (P<0.047, N=34, Wilcoxon test one-way). (B) Seasonal variation in body size (measured as WL) is summarized in the horizontal box whisker (top; see Results). Red and blue dots represent DT values of gravid mosquitoes from the DS and RS, respectively. The regression line shows the relationship of DT and WL regardless of season (see Results).

Mentions: A total of 81 gravid A. coluzzii mosquitoes were subjected to the desiccation assay in the field but only 34 intact specimens were available for morphometric and CHC analyses. They represented 23 and 11 gravid females collected during the DS and the RS, respectively. In agreement with predictions (Introduction), the mean DT of females collected in the DS (15.7) was higher than that of females collected in the RS (11.1, P<0.0003, N=81, one-way Wilcoxon test; Fig. 7A). In the subset of mosquitoes used in the morphometric and CHC analyses, however, the difference was only marginally significant (P<0.093, N=34, one-way Wilcoxon test; Fig. 7A), probably reflecting the smaller sample size.Fig. 7.


Desiccation tolerance in Anopheles coluzzii: the effects of spiracle size and cuticular hydrocarbons.

Arcaz AC, Huestis DL, Dao A, Yaro AS, Diallo M, Andersen J, Blomquist GJ, Lehmann T - J. Exp. Biol. (2016)

Seasonal difference in DT in wild gravid Anophelescoluzzii and variation in body size in relation to DT. (A) DT in the rainy season (RS) and dry season (DS). Black and blue distributions represent, respectively, the full number and a subset of specimens used in the desiccation assay and in the morphometric and CHC analyses. The seasonal difference is highly significant over the total data (P<0.0003, N=81, Wilcoxon test one-way) and significant in the subset (P<0.047, N=34, Wilcoxon test one-way). (B) Seasonal variation in body size (measured as WL) is summarized in the horizontal box whisker (top; see Results). Red and blue dots represent DT values of gravid mosquitoes from the DS and RS, respectively. The regression line shows the relationship of DT and WL regardless of season (see Results).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4920233&req=5

JEB135665F7: Seasonal difference in DT in wild gravid Anophelescoluzzii and variation in body size in relation to DT. (A) DT in the rainy season (RS) and dry season (DS). Black and blue distributions represent, respectively, the full number and a subset of specimens used in the desiccation assay and in the morphometric and CHC analyses. The seasonal difference is highly significant over the total data (P<0.0003, N=81, Wilcoxon test one-way) and significant in the subset (P<0.047, N=34, Wilcoxon test one-way). (B) Seasonal variation in body size (measured as WL) is summarized in the horizontal box whisker (top; see Results). Red and blue dots represent DT values of gravid mosquitoes from the DS and RS, respectively. The regression line shows the relationship of DT and WL regardless of season (see Results).
Mentions: A total of 81 gravid A. coluzzii mosquitoes were subjected to the desiccation assay in the field but only 34 intact specimens were available for morphometric and CHC analyses. They represented 23 and 11 gravid females collected during the DS and the RS, respectively. In agreement with predictions (Introduction), the mean DT of females collected in the DS (15.7) was higher than that of females collected in the RS (11.1, P<0.0003, N=81, one-way Wilcoxon test; Fig. 7A). In the subset of mosquitoes used in the morphometric and CHC analyses, however, the difference was only marginally significant (P<0.093, N=34, one-way Wilcoxon test; Fig. 7A), probably reflecting the smaller sample size.Fig. 7.

Bottom Line: A smaller spiracle size appeared to increase A. coluzzii's desiccation tolerance, but was not statistically significant.Seasonal changes in CHC composition were detected in Sahelian A. coluzzii Stepwise regression models suggested the effect of particular CHCs on desiccation tolerance.In conclusion, the combination of particular CHCs along with the total amount of CHCs is a primary mechanism conferring desiccation tolerance in A. coluzzii, while variation in spiracle size might be a secondary mechanism.

View Article: PubMed Central - PubMed

Affiliation: Laboratory of Malaria and Vector Research, NIAID, NIH, Rockville, MD 20852, USA.

No MeSH data available.


Related in: MedlinePlus