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Desiccation tolerance in Anopheles coluzzii: the effects of spiracle size and cuticular hydrocarbons.

Arcaz AC, Huestis DL, Dao A, Yaro AS, Diallo M, Andersen J, Blomquist GJ, Lehmann T - J. Exp. Biol. (2016)

Bottom Line: A smaller spiracle size appeared to increase A. coluzzii's desiccation tolerance, but was not statistically significant.Seasonal changes in CHC composition were detected in Sahelian A. coluzzii Stepwise regression models suggested the effect of particular CHCs on desiccation tolerance.In conclusion, the combination of particular CHCs along with the total amount of CHCs is a primary mechanism conferring desiccation tolerance in A. coluzzii, while variation in spiracle size might be a secondary mechanism.

View Article: PubMed Central - PubMed

Affiliation: Laboratory of Malaria and Vector Research, NIAID, NIH, Rockville, MD 20852, USA.

No MeSH data available.


Related in: MedlinePlus

Differences in total cuticular hydrocarbons (CHCs) between treatments. The larger variance of the dry treatment (box plot and inset) was detected by Brown and Forsythe's test of homogeneity of variance (P<0.019). The difference between means was evaluated by Welch's ANOVA. Inset shows the relationship between total CHCs and the number of CHC peaks (note the 5 extreme values). Ndry=26 and Nwet=38.
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JEB135665F5: Differences in total cuticular hydrocarbons (CHCs) between treatments. The larger variance of the dry treatment (box plot and inset) was detected by Brown and Forsythe's test of homogeneity of variance (P<0.019). The difference between means was evaluated by Welch's ANOVA. Inset shows the relationship between total CHCs and the number of CHC peaks (note the 5 extreme values). Ndry=26 and Nwet=38.

Mentions: A total of 30 CHCs were putatively identified in 64 females that were subjected to the laboratory desiccation assay. Surprisingly, the total amount of CHCs in the mosquitoes subjected to the dry treatment was higher than in those subjected to the humid treatment (P<0.006, Welch's ANOVA) and a larger variance was also evident in that group (P<0.019, Brown and Forsythe's test; Fig. 5). In part, this difference was produced by five mosquitoes (20%, Ndry=26) that had considerably larger amounts of CHCs (Fig. 5), but even excluding these individuals, the total amount of CHCs was elevated in that group, suggesting that under harsh desiccation conditions, mosquitoes can respond by elevating the total amount of CHCs (Fig. 5). However, higher amounts of total CHCs (as well as body size-adjusted total CHCs) did not increase DT or decrease RWL (P>0.35 ANCOVA, not shown).Fig. 5.


Desiccation tolerance in Anopheles coluzzii: the effects of spiracle size and cuticular hydrocarbons.

Arcaz AC, Huestis DL, Dao A, Yaro AS, Diallo M, Andersen J, Blomquist GJ, Lehmann T - J. Exp. Biol. (2016)

Differences in total cuticular hydrocarbons (CHCs) between treatments. The larger variance of the dry treatment (box plot and inset) was detected by Brown and Forsythe's test of homogeneity of variance (P<0.019). The difference between means was evaluated by Welch's ANOVA. Inset shows the relationship between total CHCs and the number of CHC peaks (note the 5 extreme values). Ndry=26 and Nwet=38.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4920233&req=5

JEB135665F5: Differences in total cuticular hydrocarbons (CHCs) between treatments. The larger variance of the dry treatment (box plot and inset) was detected by Brown and Forsythe's test of homogeneity of variance (P<0.019). The difference between means was evaluated by Welch's ANOVA. Inset shows the relationship between total CHCs and the number of CHC peaks (note the 5 extreme values). Ndry=26 and Nwet=38.
Mentions: A total of 30 CHCs were putatively identified in 64 females that were subjected to the laboratory desiccation assay. Surprisingly, the total amount of CHCs in the mosquitoes subjected to the dry treatment was higher than in those subjected to the humid treatment (P<0.006, Welch's ANOVA) and a larger variance was also evident in that group (P<0.019, Brown and Forsythe's test; Fig. 5). In part, this difference was produced by five mosquitoes (20%, Ndry=26) that had considerably larger amounts of CHCs (Fig. 5), but even excluding these individuals, the total amount of CHCs was elevated in that group, suggesting that under harsh desiccation conditions, mosquitoes can respond by elevating the total amount of CHCs (Fig. 5). However, higher amounts of total CHCs (as well as body size-adjusted total CHCs) did not increase DT or decrease RWL (P>0.35 ANCOVA, not shown).Fig. 5.

Bottom Line: A smaller spiracle size appeared to increase A. coluzzii's desiccation tolerance, but was not statistically significant.Seasonal changes in CHC composition were detected in Sahelian A. coluzzii Stepwise regression models suggested the effect of particular CHCs on desiccation tolerance.In conclusion, the combination of particular CHCs along with the total amount of CHCs is a primary mechanism conferring desiccation tolerance in A. coluzzii, while variation in spiracle size might be a secondary mechanism.

View Article: PubMed Central - PubMed

Affiliation: Laboratory of Malaria and Vector Research, NIAID, NIH, Rockville, MD 20852, USA.

No MeSH data available.


Related in: MedlinePlus