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Desiccation tolerance in Anopheles coluzzii: the effects of spiracle size and cuticular hydrocarbons.

Arcaz AC, Huestis DL, Dao A, Yaro AS, Diallo M, Andersen J, Blomquist GJ, Lehmann T - J. Exp. Biol. (2016)

Bottom Line: A smaller spiracle size appeared to increase A. coluzzii's desiccation tolerance, but was not statistically significant.Seasonal changes in CHC composition were detected in Sahelian A. coluzzii Stepwise regression models suggested the effect of particular CHCs on desiccation tolerance.In conclusion, the combination of particular CHCs along with the total amount of CHCs is a primary mechanism conferring desiccation tolerance in A. coluzzii, while variation in spiracle size might be a secondary mechanism.

View Article: PubMed Central - PubMed

Affiliation: Laboratory of Malaria and Vector Research, NIAID, NIH, Rockville, MD 20852, USA.

No MeSH data available.


Related in: MedlinePlus

Seasonal variation in CHC composition of A. coluzzii. (A) The proportion of mosquitoes exhibiting each CHC as a measure of its abundance. (B) The relative quantity of each CHC using log contrast-transformed values. Significant differences in frequency between seasons are indicated by asterisks below bars. Black and red asterisks indicate significance at the individual and multi-test level, respectively (‡P<0.1, *P<0.05, **P<0.01 and ***P<0.001). Ndry=23 and Nrainy=12.
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JEB135665F10: Seasonal variation in CHC composition of A. coluzzii. (A) The proportion of mosquitoes exhibiting each CHC as a measure of its abundance. (B) The relative quantity of each CHC using log contrast-transformed values. Significant differences in frequency between seasons are indicated by asterisks below bars. Black and red asterisks indicate significance at the individual and multi-test level, respectively (‡P<0.1, *P<0.05, **P<0.01 and ***P<0.001). Ndry=23 and Nrainy=12.

Mentions: The composition of CHCs was overall similar across seasons (Fig. 10A). Only C31:1 was season specific; it was present in all the RS specimens (N=10) but in none of the DS specimens (N=23, P<0.001, Fisher exact test; Fig. 10A). Similarly, the alkene C27:1 was present in a smaller fraction of DS mosquitoes (P<0.006, Fisher exact test; Fig. 10A), whereas 2MebrC37 and n-C28 were detected in higher proportions of DS mosquitoes (P<0.035, and P<0.054, respectively, Fisher exact test), suggesting these CHCs were more concentrated during the DS. Considering the log contrast-transformed values (Fig. 10B), significant seasonal differences in relative quantity were detected in nine of 21 CHCs, indicating a seasonal shift in composition (P<0.0001, binomial test). Further, six of the nine CHCs were also significant in the multi-test level (Fig. 10B).Fig. 10.


Desiccation tolerance in Anopheles coluzzii: the effects of spiracle size and cuticular hydrocarbons.

Arcaz AC, Huestis DL, Dao A, Yaro AS, Diallo M, Andersen J, Blomquist GJ, Lehmann T - J. Exp. Biol. (2016)

Seasonal variation in CHC composition of A. coluzzii. (A) The proportion of mosquitoes exhibiting each CHC as a measure of its abundance. (B) The relative quantity of each CHC using log contrast-transformed values. Significant differences in frequency between seasons are indicated by asterisks below bars. Black and red asterisks indicate significance at the individual and multi-test level, respectively (‡P<0.1, *P<0.05, **P<0.01 and ***P<0.001). Ndry=23 and Nrainy=12.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4920233&req=5

JEB135665F10: Seasonal variation in CHC composition of A. coluzzii. (A) The proportion of mosquitoes exhibiting each CHC as a measure of its abundance. (B) The relative quantity of each CHC using log contrast-transformed values. Significant differences in frequency between seasons are indicated by asterisks below bars. Black and red asterisks indicate significance at the individual and multi-test level, respectively (‡P<0.1, *P<0.05, **P<0.01 and ***P<0.001). Ndry=23 and Nrainy=12.
Mentions: The composition of CHCs was overall similar across seasons (Fig. 10A). Only C31:1 was season specific; it was present in all the RS specimens (N=10) but in none of the DS specimens (N=23, P<0.001, Fisher exact test; Fig. 10A). Similarly, the alkene C27:1 was present in a smaller fraction of DS mosquitoes (P<0.006, Fisher exact test; Fig. 10A), whereas 2MebrC37 and n-C28 were detected in higher proportions of DS mosquitoes (P<0.035, and P<0.054, respectively, Fisher exact test), suggesting these CHCs were more concentrated during the DS. Considering the log contrast-transformed values (Fig. 10B), significant seasonal differences in relative quantity were detected in nine of 21 CHCs, indicating a seasonal shift in composition (P<0.0001, binomial test). Further, six of the nine CHCs were also significant in the multi-test level (Fig. 10B).Fig. 10.

Bottom Line: A smaller spiracle size appeared to increase A. coluzzii's desiccation tolerance, but was not statistically significant.Seasonal changes in CHC composition were detected in Sahelian A. coluzzii Stepwise regression models suggested the effect of particular CHCs on desiccation tolerance.In conclusion, the combination of particular CHCs along with the total amount of CHCs is a primary mechanism conferring desiccation tolerance in A. coluzzii, while variation in spiracle size might be a secondary mechanism.

View Article: PubMed Central - PubMed

Affiliation: Laboratory of Malaria and Vector Research, NIAID, NIH, Rockville, MD 20852, USA.

No MeSH data available.


Related in: MedlinePlus