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Desiccation tolerance in Anopheles coluzzii: the effects of spiracle size and cuticular hydrocarbons.

Arcaz AC, Huestis DL, Dao A, Yaro AS, Diallo M, Andersen J, Blomquist GJ, Lehmann T - J. Exp. Biol. (2016)

Bottom Line: A smaller spiracle size appeared to increase A. coluzzii's desiccation tolerance, but was not statistically significant.Seasonal changes in CHC composition were detected in Sahelian A. coluzzii Stepwise regression models suggested the effect of particular CHCs on desiccation tolerance.In conclusion, the combination of particular CHCs along with the total amount of CHCs is a primary mechanism conferring desiccation tolerance in A. coluzzii, while variation in spiracle size might be a secondary mechanism.

View Article: PubMed Central - PubMed

Affiliation: Laboratory of Malaria and Vector Research, NIAID, NIH, Rockville, MD 20852, USA.

No MeSH data available.


Related in: MedlinePlus

Variation in desiccation tolerance and changes in body mass values in 3 day old G3 colony mosquitoes under dry and humid treatments. (A) Variation between treatments in desiccation tolerance (DT), measured as survival time (Ndry=39 and Nwet=44). The mean and median survival time are denoted by a diamond and a line (dividing the box), respectively. (B) Changes in body mass distribution before the assay (wet mass) and at the end of the assay (end mass), and dry mass under the dry treatment (20% relative humidity, RH). Arrows (black) point to the mean mass (, shown above the arrow with sample size) of each group. (C) Same as in B for the humid treatment (80% RH).
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JEB135665F1: Variation in desiccation tolerance and changes in body mass values in 3 day old G3 colony mosquitoes under dry and humid treatments. (A) Variation between treatments in desiccation tolerance (DT), measured as survival time (Ndry=39 and Nwet=44). The mean and median survival time are denoted by a diamond and a line (dividing the box), respectively. (B) Changes in body mass distribution before the assay (wet mass) and at the end of the assay (end mass), and dry mass under the dry treatment (20% relative humidity, RH). Arrows (black) point to the mean mass (, shown above the arrow with sample size) of each group. (C) Same as in B for the humid treatment (80% RH).

Mentions: Mosquitoes subjected to the desiccation assay survived longer in the high humidity treatment: 36.2 versus 16.3 h (P<0.0001, ANOVA, F1,95=47.8). Considerable variation in survival (DT) within treatment was indicated by the spread of the values (Fig. 1A). On average, mosquitoes lost 36% of their initial wet mass during the assay, corresponding to a reduction of ∼13% in body water content (from 78% to 65%). There was no significant difference in initial (wet) mass and in the end mass between treatments (P>0.062, F1,81>3.56, ANOVA; Fig. 1B,C); however, the dry mass of the mosquitoes subjected to the dry treatment was higher than that of the mosquitoes subjected to the humid treatment (P<0.001, F1,81>40.8, ANOVA; Fig. 1B,C), indicating that a small part of the mass lost during the assays was likely nutritional reserves rather than water.Fig. 1.


Desiccation tolerance in Anopheles coluzzii: the effects of spiracle size and cuticular hydrocarbons.

Arcaz AC, Huestis DL, Dao A, Yaro AS, Diallo M, Andersen J, Blomquist GJ, Lehmann T - J. Exp. Biol. (2016)

Variation in desiccation tolerance and changes in body mass values in 3 day old G3 colony mosquitoes under dry and humid treatments. (A) Variation between treatments in desiccation tolerance (DT), measured as survival time (Ndry=39 and Nwet=44). The mean and median survival time are denoted by a diamond and a line (dividing the box), respectively. (B) Changes in body mass distribution before the assay (wet mass) and at the end of the assay (end mass), and dry mass under the dry treatment (20% relative humidity, RH). Arrows (black) point to the mean mass (, shown above the arrow with sample size) of each group. (C) Same as in B for the humid treatment (80% RH).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4920233&req=5

JEB135665F1: Variation in desiccation tolerance and changes in body mass values in 3 day old G3 colony mosquitoes under dry and humid treatments. (A) Variation between treatments in desiccation tolerance (DT), measured as survival time (Ndry=39 and Nwet=44). The mean and median survival time are denoted by a diamond and a line (dividing the box), respectively. (B) Changes in body mass distribution before the assay (wet mass) and at the end of the assay (end mass), and dry mass under the dry treatment (20% relative humidity, RH). Arrows (black) point to the mean mass (, shown above the arrow with sample size) of each group. (C) Same as in B for the humid treatment (80% RH).
Mentions: Mosquitoes subjected to the desiccation assay survived longer in the high humidity treatment: 36.2 versus 16.3 h (P<0.0001, ANOVA, F1,95=47.8). Considerable variation in survival (DT) within treatment was indicated by the spread of the values (Fig. 1A). On average, mosquitoes lost 36% of their initial wet mass during the assay, corresponding to a reduction of ∼13% in body water content (from 78% to 65%). There was no significant difference in initial (wet) mass and in the end mass between treatments (P>0.062, F1,81>3.56, ANOVA; Fig. 1B,C); however, the dry mass of the mosquitoes subjected to the dry treatment was higher than that of the mosquitoes subjected to the humid treatment (P<0.001, F1,81>40.8, ANOVA; Fig. 1B,C), indicating that a small part of the mass lost during the assays was likely nutritional reserves rather than water.Fig. 1.

Bottom Line: A smaller spiracle size appeared to increase A. coluzzii's desiccation tolerance, but was not statistically significant.Seasonal changes in CHC composition were detected in Sahelian A. coluzzii Stepwise regression models suggested the effect of particular CHCs on desiccation tolerance.In conclusion, the combination of particular CHCs along with the total amount of CHCs is a primary mechanism conferring desiccation tolerance in A. coluzzii, while variation in spiracle size might be a secondary mechanism.

View Article: PubMed Central - PubMed

Affiliation: Laboratory of Malaria and Vector Research, NIAID, NIH, Rockville, MD 20852, USA.

No MeSH data available.


Related in: MedlinePlus