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Roles of microRNA-99 family in human glioma.

Zhang M, Guo Y, Wu J, Chen F, Dai Z, Fan S, Li P, Song T - Onco Targets Ther (2016)

Bottom Line: Deregulation of microRNA (miR)-99 family members (miR-99a, miR-99b, and miR-100) has been reported to play a crucial role in many cancer types.Compared with non-neoplastic brain tissues, miR-99a, miR-99b, and miR-100 expression levels were all significantly decreased in glioma tissues (all P<0.001). miR-99a-low, miR-99b-low, and miR-100-low expression more frequently occurred in glioma patients with low Karnofsky performance score (<90) and high World Health Organization grade (III-IV).Our results strongly suggest that the aberrant expression of miR-99a, miR-99b, and miR-100 may be a common feature in human gliomas with aggressive clinicopathological features and may participate in malignant phenotypes of the tumors.

View Article: PubMed Central - PubMed

Affiliation: Department of Neurosurgery, Xiangya Hospital of Central South University, Changsha.

ABSTRACT

Objective: Deregulation of microRNA (miR)-99 family members (miR-99a, miR-99b, and miR-100) has been reported to play a crucial role in many cancer types. However, their roles in human gliomas have not been fully elucidated. This study aimed to investigate the expression patterns of miR-99a, miR-99b, and miR-100 in glioma tissues and to evaluate their expression profiles with respect to tumor progression.

Methods: Quantitative real-time polymerase chain reaction was performed to detect the expression levels of miR-99a, miR-99b, and miR-100 in glioma and matched non-neoplastic brain tissues. Then, the associations of their expression with various clinicopathological features of glioma patients were statistically analyzed. Moreover, the roles of miR-99a, miR-99b, and miR-100 in regulating glioma cell migration and invasion were determined via transwell assay in vitro.

Results: Compared with non-neoplastic brain tissues, miR-99a, miR-99b, and miR-100 expression levels were all significantly decreased in glioma tissues (all P<0.001). miR-99a-low, miR-99b-low, and miR-100-low expression more frequently occurred in glioma patients with low Karnofsky performance score (<90) and high World Health Organization grade (III-IV). Further functional experiments revealed that the enforced expression of miR-99a, miR-99b, and miR-100 resulted in the inhibition of cellular migration and invasion in glioma cells.

Conclusion: Our results strongly suggest that the aberrant expression of miR-99a, miR-99b, and miR-100 may be a common feature in human gliomas with aggressive clinicopathological features and may participate in malignant phenotypes of the tumors. These findings highlight the potential of the three miR-99 family members as novel therapeutic targets for human gliomas.

No MeSH data available.


Related in: MedlinePlus

Relative expression levels of miR-99a (A), miR-99b (B), and miR-100 (C) in human glioma and non-neoplastic brain tissues.Abbreviation: miR, microRNA.
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f1-ott-9-3613: Relative expression levels of miR-99a (A), miR-99b (B), and miR-100 (C) in human glioma and non-neoplastic brain tissues.Abbreviation: miR, microRNA.

Mentions: Compared with non-neoplastic brain tissues, miR-99a (tumor vs normal: 2.67±1.07 vs 4.67±1.13, P<0.001), miR-99b (tumor vs normal: 2.57±0.91 vs 5.26±1.19, P<0.001), and miR-100 (tumor vs normal: 2.41±1.02 vs 5.74±0.81, P<0.001) expression levels were all significantly decreased in glioma tissues (Figure 1).


Roles of microRNA-99 family in human glioma.

Zhang M, Guo Y, Wu J, Chen F, Dai Z, Fan S, Li P, Song T - Onco Targets Ther (2016)

Relative expression levels of miR-99a (A), miR-99b (B), and miR-100 (C) in human glioma and non-neoplastic brain tissues.Abbreviation: miR, microRNA.
© Copyright Policy
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4920231&req=5

f1-ott-9-3613: Relative expression levels of miR-99a (A), miR-99b (B), and miR-100 (C) in human glioma and non-neoplastic brain tissues.Abbreviation: miR, microRNA.
Mentions: Compared with non-neoplastic brain tissues, miR-99a (tumor vs normal: 2.67±1.07 vs 4.67±1.13, P<0.001), miR-99b (tumor vs normal: 2.57±0.91 vs 5.26±1.19, P<0.001), and miR-100 (tumor vs normal: 2.41±1.02 vs 5.74±0.81, P<0.001) expression levels were all significantly decreased in glioma tissues (Figure 1).

Bottom Line: Deregulation of microRNA (miR)-99 family members (miR-99a, miR-99b, and miR-100) has been reported to play a crucial role in many cancer types.Compared with non-neoplastic brain tissues, miR-99a, miR-99b, and miR-100 expression levels were all significantly decreased in glioma tissues (all P<0.001). miR-99a-low, miR-99b-low, and miR-100-low expression more frequently occurred in glioma patients with low Karnofsky performance score (<90) and high World Health Organization grade (III-IV).Our results strongly suggest that the aberrant expression of miR-99a, miR-99b, and miR-100 may be a common feature in human gliomas with aggressive clinicopathological features and may participate in malignant phenotypes of the tumors.

View Article: PubMed Central - PubMed

Affiliation: Department of Neurosurgery, Xiangya Hospital of Central South University, Changsha.

ABSTRACT

Objective: Deregulation of microRNA (miR)-99 family members (miR-99a, miR-99b, and miR-100) has been reported to play a crucial role in many cancer types. However, their roles in human gliomas have not been fully elucidated. This study aimed to investigate the expression patterns of miR-99a, miR-99b, and miR-100 in glioma tissues and to evaluate their expression profiles with respect to tumor progression.

Methods: Quantitative real-time polymerase chain reaction was performed to detect the expression levels of miR-99a, miR-99b, and miR-100 in glioma and matched non-neoplastic brain tissues. Then, the associations of their expression with various clinicopathological features of glioma patients were statistically analyzed. Moreover, the roles of miR-99a, miR-99b, and miR-100 in regulating glioma cell migration and invasion were determined via transwell assay in vitro.

Results: Compared with non-neoplastic brain tissues, miR-99a, miR-99b, and miR-100 expression levels were all significantly decreased in glioma tissues (all P<0.001). miR-99a-low, miR-99b-low, and miR-100-low expression more frequently occurred in glioma patients with low Karnofsky performance score (<90) and high World Health Organization grade (III-IV). Further functional experiments revealed that the enforced expression of miR-99a, miR-99b, and miR-100 resulted in the inhibition of cellular migration and invasion in glioma cells.

Conclusion: Our results strongly suggest that the aberrant expression of miR-99a, miR-99b, and miR-100 may be a common feature in human gliomas with aggressive clinicopathological features and may participate in malignant phenotypes of the tumors. These findings highlight the potential of the three miR-99 family members as novel therapeutic targets for human gliomas.

No MeSH data available.


Related in: MedlinePlus