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Sipa1l1 is an early biomarker of liver fibrosis in CCl4-treated rats.

Marfà S, Morales-Ruiz M, Oró D, Ribera J, Fernández-Varo G, Jiménez W - Biol Open (2016)

Bottom Line: However, these methods may involve clinical complications and/or present diagnostic uncertainty mainly in the early stages of the disease.In cirrhosis, more biological imbalances were detected as well as multi-organ alterations.In addition, hemopexin and signal-induced proliferation-associated 1 like 1 (SIPA1L1) were selected as potential serum markers of liver fibrogenesis among all the analyzed proteins.

View Article: PubMed Central - PubMed

Affiliation: Biochemistry and Molecular Genetics Service, Hospital Clinic, Centro de Investigación Biomédica en Red de Enfermedades Hepáticas y Digestivas (CIBEREHD), Institut d'Investigacions Biomèdiques August Pi i Sunyer (IDIBAPS), 08036 Barcelona, Spain.

No MeSH data available.


Related in: MedlinePlus

Diagnostic accuracy of hemopexin and SIPA1L1 and diagnostic tests of SIPA1L1. (A) Diagnostic accuracy of hemopexin and SIPA1L1 to differentiate cirrhosis and fibrosis, respectively. When using the hemopexin serum concentration, the AUROC was 0.702 but not statistically significant (P>0.05). The AUROC obtained from SIPA1L1 serum concentration was 0.865 and statistically significant (P<0.0001). (B) Several diagnostic tests (Sensitivity, Specificity, NPV and PPV) were performed in SIPA1L1 after determining 475.5 pg/ml as the optimal cutoff.
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BIO018887F5: Diagnostic accuracy of hemopexin and SIPA1L1 and diagnostic tests of SIPA1L1. (A) Diagnostic accuracy of hemopexin and SIPA1L1 to differentiate cirrhosis and fibrosis, respectively. When using the hemopexin serum concentration, the AUROC was 0.702 but not statistically significant (P>0.05). The AUROC obtained from SIPA1L1 serum concentration was 0.865 and statistically significant (P<0.0001). (B) Several diagnostic tests (Sensitivity, Specificity, NPV and PPV) were performed in SIPA1L1 after determining 475.5 pg/ml as the optimal cutoff.

Mentions: The results for the accuracy of SIPA1L1 and hemopexin in fibrotic and cirrhotic rats are presented as receiver-operating characteristic (ROC) curves in Fig. 5A. Animals belonging to the fibrotic groups (n=50) were considered in the analysis for SIPA1L1, whereas only cirrhotic rats were considered at assessing hemopexin (n=17). The ROC of SIPA1L1 showed an excellent diagnostic accuracy to discriminate rats with fibrosis from control animals [area under the ROC (AUROC): 0.865, P<0.0001]. In contrast, the ROC of hemopexin to discriminate cirrhotic from control rats displayed a considerably lower diagnostic efficacy (AUROC: 0.702), which lacked statistical significance. In addition, a serum concentration of SIPAL1L1 of 475.5 pg/ml was selected as the optimal cutoff value to differentiate normal from fibrotic animals. This estimation was based on the maximum value of the likelihood ratio, which minimizes the number of false positive and false negative cases. Above this cutoff, 86% of rats did not show significant fibrosis. Below this cutoff, 97% of rats had fibrosis. In addition, we correctly classified 74% of the animals with fibrosis. Finally, the specificity was also determined and reached the 95% (Fig. 5B).Fig. 5.


Sipa1l1 is an early biomarker of liver fibrosis in CCl4-treated rats.

Marfà S, Morales-Ruiz M, Oró D, Ribera J, Fernández-Varo G, Jiménez W - Biol Open (2016)

Diagnostic accuracy of hemopexin and SIPA1L1 and diagnostic tests of SIPA1L1. (A) Diagnostic accuracy of hemopexin and SIPA1L1 to differentiate cirrhosis and fibrosis, respectively. When using the hemopexin serum concentration, the AUROC was 0.702 but not statistically significant (P>0.05). The AUROC obtained from SIPA1L1 serum concentration was 0.865 and statistically significant (P<0.0001). (B) Several diagnostic tests (Sensitivity, Specificity, NPV and PPV) were performed in SIPA1L1 after determining 475.5 pg/ml as the optimal cutoff.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4920198&req=5

BIO018887F5: Diagnostic accuracy of hemopexin and SIPA1L1 and diagnostic tests of SIPA1L1. (A) Diagnostic accuracy of hemopexin and SIPA1L1 to differentiate cirrhosis and fibrosis, respectively. When using the hemopexin serum concentration, the AUROC was 0.702 but not statistically significant (P>0.05). The AUROC obtained from SIPA1L1 serum concentration was 0.865 and statistically significant (P<0.0001). (B) Several diagnostic tests (Sensitivity, Specificity, NPV and PPV) were performed in SIPA1L1 after determining 475.5 pg/ml as the optimal cutoff.
Mentions: The results for the accuracy of SIPA1L1 and hemopexin in fibrotic and cirrhotic rats are presented as receiver-operating characteristic (ROC) curves in Fig. 5A. Animals belonging to the fibrotic groups (n=50) were considered in the analysis for SIPA1L1, whereas only cirrhotic rats were considered at assessing hemopexin (n=17). The ROC of SIPA1L1 showed an excellent diagnostic accuracy to discriminate rats with fibrosis from control animals [area under the ROC (AUROC): 0.865, P<0.0001]. In contrast, the ROC of hemopexin to discriminate cirrhotic from control rats displayed a considerably lower diagnostic efficacy (AUROC: 0.702), which lacked statistical significance. In addition, a serum concentration of SIPAL1L1 of 475.5 pg/ml was selected as the optimal cutoff value to differentiate normal from fibrotic animals. This estimation was based on the maximum value of the likelihood ratio, which minimizes the number of false positive and false negative cases. Above this cutoff, 86% of rats did not show significant fibrosis. Below this cutoff, 97% of rats had fibrosis. In addition, we correctly classified 74% of the animals with fibrosis. Finally, the specificity was also determined and reached the 95% (Fig. 5B).Fig. 5.

Bottom Line: However, these methods may involve clinical complications and/or present diagnostic uncertainty mainly in the early stages of the disease.In cirrhosis, more biological imbalances were detected as well as multi-organ alterations.In addition, hemopexin and signal-induced proliferation-associated 1 like 1 (SIPA1L1) were selected as potential serum markers of liver fibrogenesis among all the analyzed proteins.

View Article: PubMed Central - PubMed

Affiliation: Biochemistry and Molecular Genetics Service, Hospital Clinic, Centro de Investigación Biomédica en Red de Enfermedades Hepáticas y Digestivas (CIBEREHD), Institut d'Investigacions Biomèdiques August Pi i Sunyer (IDIBAPS), 08036 Barcelona, Spain.

No MeSH data available.


Related in: MedlinePlus