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Sipa1l1 is an early biomarker of liver fibrosis in CCl4-treated rats.

Marfà S, Morales-Ruiz M, Oró D, Ribera J, Fernández-Varo G, Jiménez W - Biol Open (2016)

Bottom Line: However, these methods may involve clinical complications and/or present diagnostic uncertainty mainly in the early stages of the disease.In cirrhosis, more biological imbalances were detected as well as multi-organ alterations.In addition, hemopexin and signal-induced proliferation-associated 1 like 1 (SIPA1L1) were selected as potential serum markers of liver fibrogenesis among all the analyzed proteins.

View Article: PubMed Central - PubMed

Affiliation: Biochemistry and Molecular Genetics Service, Hospital Clinic, Centro de Investigación Biomédica en Red de Enfermedades Hepáticas y Digestivas (CIBEREHD), Institut d'Investigacions Biomèdiques August Pi i Sunyer (IDIBAPS), 08036 Barcelona, Spain.

No MeSH data available.


Related in: MedlinePlus

MS/MS analysis of the SIPA1L1 and hemopexin peptides identified. (A) Amino acid sequencing of SIPA1L1 and hemopexin. (B,C) Representative MS/MS spectra of the peptides identified from SIPA1L1 (B) and hemopexin (C).
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BIO018887F3: MS/MS analysis of the SIPA1L1 and hemopexin peptides identified. (A) Amino acid sequencing of SIPA1L1 and hemopexin. (B,C) Representative MS/MS spectra of the peptides identified from SIPA1L1 (B) and hemopexin (C).

Mentions: The ten different protein peaks showing the most statistically significant expression in samples from CCl4-treated rats as compared to controls were selected. Among these, four were specifically detected in samples from fibrotic animals whereas six were exclusively found in cirrhotic rats (Table 2). Only proteins showing at least a twofold change in expression were further considered for subsequent analysis. Moreover, high-abundant serum proteins and proteins not very conserved between rats and humans were also excluded. According to this data processing strategy, only SIPA1L1 and hemopexin were selected. In particular, SIPA1L1 was down expressed in fibrosis whereas hemopexin was increased in cirrhosis. Representative MS/MS spectra from the SIPA1L1 and hemopexin peptides are shown in Fig. 3.Table 2.


Sipa1l1 is an early biomarker of liver fibrosis in CCl4-treated rats.

Marfà S, Morales-Ruiz M, Oró D, Ribera J, Fernández-Varo G, Jiménez W - Biol Open (2016)

MS/MS analysis of the SIPA1L1 and hemopexin peptides identified. (A) Amino acid sequencing of SIPA1L1 and hemopexin. (B,C) Representative MS/MS spectra of the peptides identified from SIPA1L1 (B) and hemopexin (C).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4920198&req=5

BIO018887F3: MS/MS analysis of the SIPA1L1 and hemopexin peptides identified. (A) Amino acid sequencing of SIPA1L1 and hemopexin. (B,C) Representative MS/MS spectra of the peptides identified from SIPA1L1 (B) and hemopexin (C).
Mentions: The ten different protein peaks showing the most statistically significant expression in samples from CCl4-treated rats as compared to controls were selected. Among these, four were specifically detected in samples from fibrotic animals whereas six were exclusively found in cirrhotic rats (Table 2). Only proteins showing at least a twofold change in expression were further considered for subsequent analysis. Moreover, high-abundant serum proteins and proteins not very conserved between rats and humans were also excluded. According to this data processing strategy, only SIPA1L1 and hemopexin were selected. In particular, SIPA1L1 was down expressed in fibrosis whereas hemopexin was increased in cirrhosis. Representative MS/MS spectra from the SIPA1L1 and hemopexin peptides are shown in Fig. 3.Table 2.

Bottom Line: However, these methods may involve clinical complications and/or present diagnostic uncertainty mainly in the early stages of the disease.In cirrhosis, more biological imbalances were detected as well as multi-organ alterations.In addition, hemopexin and signal-induced proliferation-associated 1 like 1 (SIPA1L1) were selected as potential serum markers of liver fibrogenesis among all the analyzed proteins.

View Article: PubMed Central - PubMed

Affiliation: Biochemistry and Molecular Genetics Service, Hospital Clinic, Centro de Investigación Biomédica en Red de Enfermedades Hepáticas y Digestivas (CIBEREHD), Institut d'Investigacions Biomèdiques August Pi i Sunyer (IDIBAPS), 08036 Barcelona, Spain.

No MeSH data available.


Related in: MedlinePlus