Limits...
Spermidine, but not spermine, is essential for pigment pattern formation in zebrafish.

Frohnhöfer HG, Geiger-Rudolph S, Pattky M, Meixner M, Huhn C, Maischein HM, Geisler R, Gehring I, Maderspacher F, Nüsslein-Volhard C, Irion U - Biol Open (2016)

Bottom Line: Here we identify idefix, a mutation in the zebrafish gene encoding the enzyme spermidine synthase, leading to a severe reduction in spermidine levels as shown by capillary electrophoresis-mass spectrometry.This allows us to uncouple them from events occurring later during colour patterning.Thus, zebrafish provide a vertebrate model to study the in vivo effects of polyamines.

View Article: PubMed Central - PubMed

Affiliation: Max-Planck-Institut für Entwicklungsbiologie, Abteilung 3, Spemannstrasse 35, Tübingen 72076, Germany.

No MeSH data available.


Related in: MedlinePlus

idefix mutants show a maternal-effect lethal phenotype. Wild-type embryos and larvae (A-C) and maternal-zygotic idefix mutants (D-I, ide−/− mat. & zyg.) derived from an incross of two homozygous fish are shown at 4 h post fertilization (hpf), 26 hpf and 3 days post fertilization (dpf). A variable proportion of the mutants show morphological defects, which become apparent at different developmental stages; examples were selected to illustrate the most severe phenotypes. Scale bars: 0.5 mm.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4920196&req=5

BIO018721F5: idefix mutants show a maternal-effect lethal phenotype. Wild-type embryos and larvae (A-C) and maternal-zygotic idefix mutants (D-I, ide−/− mat. & zyg.) derived from an incross of two homozygous fish are shown at 4 h post fertilization (hpf), 26 hpf and 3 days post fertilization (dpf). A variable proportion of the mutants show morphological defects, which become apparent at different developmental stages; examples were selected to illustrate the most severe phenotypes. Scale bars: 0.5 mm.

Mentions: In addition to pigment patterning defects, ide mutants show a maternal-effect lethal phenotype. Homozygous mutant larvae derived from homozygous mothers (maternal-zygotic mutants) never survive beyond day 7. They display a large range of different phenotypes, with morphological defects becoming visible in a varying number of embryos during different stages of development. The earliest defects are apparent already during the first few hours of development and some embryos do not complete epiboly and gastrulation. However, often many of them look normal initially and only show defects during the next few days of development, e.g. patterning defects and malformations of the head, heart oedema or curved bodies (Fig. 5A-I). None of the larvae inflate the swim bladder and they do not start feeding.Fig. 5.


Spermidine, but not spermine, is essential for pigment pattern formation in zebrafish.

Frohnhöfer HG, Geiger-Rudolph S, Pattky M, Meixner M, Huhn C, Maischein HM, Geisler R, Gehring I, Maderspacher F, Nüsslein-Volhard C, Irion U - Biol Open (2016)

idefix mutants show a maternal-effect lethal phenotype. Wild-type embryos and larvae (A-C) and maternal-zygotic idefix mutants (D-I, ide−/− mat. & zyg.) derived from an incross of two homozygous fish are shown at 4 h post fertilization (hpf), 26 hpf and 3 days post fertilization (dpf). A variable proportion of the mutants show morphological defects, which become apparent at different developmental stages; examples were selected to illustrate the most severe phenotypes. Scale bars: 0.5 mm.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4920196&req=5

BIO018721F5: idefix mutants show a maternal-effect lethal phenotype. Wild-type embryos and larvae (A-C) and maternal-zygotic idefix mutants (D-I, ide−/− mat. & zyg.) derived from an incross of two homozygous fish are shown at 4 h post fertilization (hpf), 26 hpf and 3 days post fertilization (dpf). A variable proportion of the mutants show morphological defects, which become apparent at different developmental stages; examples were selected to illustrate the most severe phenotypes. Scale bars: 0.5 mm.
Mentions: In addition to pigment patterning defects, ide mutants show a maternal-effect lethal phenotype. Homozygous mutant larvae derived from homozygous mothers (maternal-zygotic mutants) never survive beyond day 7. They display a large range of different phenotypes, with morphological defects becoming visible in a varying number of embryos during different stages of development. The earliest defects are apparent already during the first few hours of development and some embryos do not complete epiboly and gastrulation. However, often many of them look normal initially and only show defects during the next few days of development, e.g. patterning defects and malformations of the head, heart oedema or curved bodies (Fig. 5A-I). None of the larvae inflate the swim bladder and they do not start feeding.Fig. 5.

Bottom Line: Here we identify idefix, a mutation in the zebrafish gene encoding the enzyme spermidine synthase, leading to a severe reduction in spermidine levels as shown by capillary electrophoresis-mass spectrometry.This allows us to uncouple them from events occurring later during colour patterning.Thus, zebrafish provide a vertebrate model to study the in vivo effects of polyamines.

View Article: PubMed Central - PubMed

Affiliation: Max-Planck-Institut für Entwicklungsbiologie, Abteilung 3, Spemannstrasse 35, Tübingen 72076, Germany.

No MeSH data available.


Related in: MedlinePlus