Limits...
Spermidine, but not spermine, is essential for pigment pattern formation in zebrafish.

Frohnhöfer HG, Geiger-Rudolph S, Pattky M, Meixner M, Huhn C, Maischein HM, Geisler R, Gehring I, Maderspacher F, Nüsslein-Volhard C, Irion U - Biol Open (2016)

Bottom Line: Here we identify idefix, a mutation in the zebrafish gene encoding the enzyme spermidine synthase, leading to a severe reduction in spermidine levels as shown by capillary electrophoresis-mass spectrometry.This allows us to uncouple them from events occurring later during colour patterning.Thus, zebrafish provide a vertebrate model to study the in vivo effects of polyamines.

View Article: PubMed Central - PubMed

Affiliation: Max-Planck-Institut für Entwicklungsbiologie, Abteilung 3, Spemannstrasse 35, Tübingen 72076, Germany.

No MeSH data available.


Related in: MedlinePlus

idefix mutant chromatophores contribute to a wild-type pattern in chimeric animals. Chimeric animals derived from blastomere transplantations of ide mutant cells into (A) pfeffer (n=8), (B) rose (n=6) and (C) nacre (n=4) hosts. In all three cases the normally striped wild-type pattern is restored in the regions of donor-derived xanthophores (A), iridophores (B) or melanophores (C), indicated by the brackets. Scale bar: 1 mm.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4920196&req=5

BIO018721F3: idefix mutant chromatophores contribute to a wild-type pattern in chimeric animals. Chimeric animals derived from blastomere transplantations of ide mutant cells into (A) pfeffer (n=8), (B) rose (n=6) and (C) nacre (n=4) hosts. In all three cases the normally striped wild-type pattern is restored in the regions of donor-derived xanthophores (A), iridophores (B) or melanophores (C), indicated by the brackets. Scale bar: 1 mm.

Mentions: To test whether the function of ide is autonomously required in pigment cells we created chimeric animals by transplanting blastomeres from homozygous ide mutant donor embryos into pfeffer, rose or nacre hosts, which specifically lack xanthophores, iridophores or melanophores, respectively, and therefore display only a residual, abnormal stripe pattern. In all three cases we found that the ide mutant chromatophores can restore the wild-type pattern in the chimeric animals and generate stripes of normal width (Fig. 3). This demonstrates that the gene function of ide is not required in pigment cells, but rather influences their behaviour indirectly.Fig. 3.


Spermidine, but not spermine, is essential for pigment pattern formation in zebrafish.

Frohnhöfer HG, Geiger-Rudolph S, Pattky M, Meixner M, Huhn C, Maischein HM, Geisler R, Gehring I, Maderspacher F, Nüsslein-Volhard C, Irion U - Biol Open (2016)

idefix mutant chromatophores contribute to a wild-type pattern in chimeric animals. Chimeric animals derived from blastomere transplantations of ide mutant cells into (A) pfeffer (n=8), (B) rose (n=6) and (C) nacre (n=4) hosts. In all three cases the normally striped wild-type pattern is restored in the regions of donor-derived xanthophores (A), iridophores (B) or melanophores (C), indicated by the brackets. Scale bar: 1 mm.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4920196&req=5

BIO018721F3: idefix mutant chromatophores contribute to a wild-type pattern in chimeric animals. Chimeric animals derived from blastomere transplantations of ide mutant cells into (A) pfeffer (n=8), (B) rose (n=6) and (C) nacre (n=4) hosts. In all three cases the normally striped wild-type pattern is restored in the regions of donor-derived xanthophores (A), iridophores (B) or melanophores (C), indicated by the brackets. Scale bar: 1 mm.
Mentions: To test whether the function of ide is autonomously required in pigment cells we created chimeric animals by transplanting blastomeres from homozygous ide mutant donor embryos into pfeffer, rose or nacre hosts, which specifically lack xanthophores, iridophores or melanophores, respectively, and therefore display only a residual, abnormal stripe pattern. In all three cases we found that the ide mutant chromatophores can restore the wild-type pattern in the chimeric animals and generate stripes of normal width (Fig. 3). This demonstrates that the gene function of ide is not required in pigment cells, but rather influences their behaviour indirectly.Fig. 3.

Bottom Line: Here we identify idefix, a mutation in the zebrafish gene encoding the enzyme spermidine synthase, leading to a severe reduction in spermidine levels as shown by capillary electrophoresis-mass spectrometry.This allows us to uncouple them from events occurring later during colour patterning.Thus, zebrafish provide a vertebrate model to study the in vivo effects of polyamines.

View Article: PubMed Central - PubMed

Affiliation: Max-Planck-Institut für Entwicklungsbiologie, Abteilung 3, Spemannstrasse 35, Tübingen 72076, Germany.

No MeSH data available.


Related in: MedlinePlus