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Spermidine, but not spermine, is essential for pigment pattern formation in zebrafish.

Frohnhöfer HG, Geiger-Rudolph S, Pattky M, Meixner M, Huhn C, Maischein HM, Geisler R, Gehring I, Maderspacher F, Nüsslein-Volhard C, Irion U - Biol Open (2016)

Bottom Line: Here we identify idefix, a mutation in the zebrafish gene encoding the enzyme spermidine synthase, leading to a severe reduction in spermidine levels as shown by capillary electrophoresis-mass spectrometry.This allows us to uncouple them from events occurring later during colour patterning.Thus, zebrafish provide a vertebrate model to study the in vivo effects of polyamines.

View Article: PubMed Central - PubMed

Affiliation: Max-Planck-Institut für Entwicklungsbiologie, Abteilung 3, Spemannstrasse 35, Tübingen 72076, Germany.

No MeSH data available.


Related in: MedlinePlus

idefix mutants show defects in pigment pattern formation. Wild-type (A,A′) zebrafish show a stereotypic pattern of horizontal dark and light stripes on their flanks and on the anal and caudal fins. In ide mutants (B,B′) the light stripe areas are expanded, there are fewer and less regular dark stripes, which frequently show interruptions. The striped pattern in the anal and caudal fins is also disrupted in ide mutants. Double mutants of ide with leo (C-D′), luc (E-F′) or obe heterozygous (G-H′) and homozygous (I-J′) show a superimposition of both phenotypes. The light stripe areas are expanded in all cases. Scale bars: 5 mm J, 1 mm in J′.
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BIO018721F1: idefix mutants show defects in pigment pattern formation. Wild-type (A,A′) zebrafish show a stereotypic pattern of horizontal dark and light stripes on their flanks and on the anal and caudal fins. In ide mutants (B,B′) the light stripe areas are expanded, there are fewer and less regular dark stripes, which frequently show interruptions. The striped pattern in the anal and caudal fins is also disrupted in ide mutants. Double mutants of ide with leo (C-D′), luc (E-F′) or obe heterozygous (G-H′) and homozygous (I-J′) show a superimposition of both phenotypes. The light stripe areas are expanded in all cases. Scale bars: 5 mm J, 1 mm in J′.

Mentions: During an ENU-mutagenesis and screen for mutations causing developmental defects in zebrafish larvae we serendipitously identified one recessive mutant where the stripe width of the adult fish is altered. We named this mutant idefix (ide), based on its superficial resemblance to two other mutants with defects in stripe width, obelix (obe, also known as jaguar) and asterix (ase). Homozygous ide mutants have fewer dark stripes, only 2-3 compared to 4-5 in wild type, in addition these stripes are less regular, usually narrower and often interrupted. The stripes in the anal and tail fins are also affected, they are only partially present in ide mutants (Fig. 1A,B). In double mutants, where ide is combined with leo, luc or obe, a superimposition of both phenotypes is visible. The light stripe areas are expanded between the spots or broadened dark stripes characteristic for the single mutants (Fig. 1C-J). These findings show that ide does not act exclusively through one of the pathways defined by obe or leo and luc; otherwise the phenotype of the loss-of-function mutants would not be altered in the double mutants.Fig. 1.


Spermidine, but not spermine, is essential for pigment pattern formation in zebrafish.

Frohnhöfer HG, Geiger-Rudolph S, Pattky M, Meixner M, Huhn C, Maischein HM, Geisler R, Gehring I, Maderspacher F, Nüsslein-Volhard C, Irion U - Biol Open (2016)

idefix mutants show defects in pigment pattern formation. Wild-type (A,A′) zebrafish show a stereotypic pattern of horizontal dark and light stripes on their flanks and on the anal and caudal fins. In ide mutants (B,B′) the light stripe areas are expanded, there are fewer and less regular dark stripes, which frequently show interruptions. The striped pattern in the anal and caudal fins is also disrupted in ide mutants. Double mutants of ide with leo (C-D′), luc (E-F′) or obe heterozygous (G-H′) and homozygous (I-J′) show a superimposition of both phenotypes. The light stripe areas are expanded in all cases. Scale bars: 5 mm J, 1 mm in J′.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4920196&req=5

BIO018721F1: idefix mutants show defects in pigment pattern formation. Wild-type (A,A′) zebrafish show a stereotypic pattern of horizontal dark and light stripes on their flanks and on the anal and caudal fins. In ide mutants (B,B′) the light stripe areas are expanded, there are fewer and less regular dark stripes, which frequently show interruptions. The striped pattern in the anal and caudal fins is also disrupted in ide mutants. Double mutants of ide with leo (C-D′), luc (E-F′) or obe heterozygous (G-H′) and homozygous (I-J′) show a superimposition of both phenotypes. The light stripe areas are expanded in all cases. Scale bars: 5 mm J, 1 mm in J′.
Mentions: During an ENU-mutagenesis and screen for mutations causing developmental defects in zebrafish larvae we serendipitously identified one recessive mutant where the stripe width of the adult fish is altered. We named this mutant idefix (ide), based on its superficial resemblance to two other mutants with defects in stripe width, obelix (obe, also known as jaguar) and asterix (ase). Homozygous ide mutants have fewer dark stripes, only 2-3 compared to 4-5 in wild type, in addition these stripes are less regular, usually narrower and often interrupted. The stripes in the anal and tail fins are also affected, they are only partially present in ide mutants (Fig. 1A,B). In double mutants, where ide is combined with leo, luc or obe, a superimposition of both phenotypes is visible. The light stripe areas are expanded between the spots or broadened dark stripes characteristic for the single mutants (Fig. 1C-J). These findings show that ide does not act exclusively through one of the pathways defined by obe or leo and luc; otherwise the phenotype of the loss-of-function mutants would not be altered in the double mutants.Fig. 1.

Bottom Line: Here we identify idefix, a mutation in the zebrafish gene encoding the enzyme spermidine synthase, leading to a severe reduction in spermidine levels as shown by capillary electrophoresis-mass spectrometry.This allows us to uncouple them from events occurring later during colour patterning.Thus, zebrafish provide a vertebrate model to study the in vivo effects of polyamines.

View Article: PubMed Central - PubMed

Affiliation: Max-Planck-Institut für Entwicklungsbiologie, Abteilung 3, Spemannstrasse 35, Tübingen 72076, Germany.

No MeSH data available.


Related in: MedlinePlus