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Quantitative analysis of 3D extracellular matrix remodelling by pancreatic stellate cells.

Robinson BK, Cortes E, Rice AJ, Sarper M, Del Río Hernández A - Biol Open (2016)

Bottom Line: We present an integrated methodology where cell-ECM interactions can be investigated in 3D environments via ECM remodelling.Pancreatic stellate cells (PSCs) are the key effectors of the stromal fibrosis associated to pancreatic cancer.We use PSCs to implement our methodology and demonstrate that PSC matrix remodelling capabilities depend on their contractile machinery and β1 integrin-mediated cell-ECM attachment.

View Article: PubMed Central - PubMed

Affiliation: Cellular and Molecular Biomechanics Laboratory, Department of Bioengineering, Faculty of Engineering, Imperial College London, South Kensington Campus, London SW7 2AZ, UK.

No MeSH data available.


Related in: MedlinePlus

Representation of SHG signal generation from collagen-I. (A) The peptide bonds of the collagen chains create a permanent dipole moment along the triple helix that allows second harmonic generation. (B) Jablonski diagram of SHG. Excitation with two photons with identical energy E, leads to a virtual energy state and emission of a photon with energy 2E.
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BIO017632F2: Representation of SHG signal generation from collagen-I. (A) The peptide bonds of the collagen chains create a permanent dipole moment along the triple helix that allows second harmonic generation. (B) Jablonski diagram of SHG. Excitation with two photons with identical energy E, leads to a virtual energy state and emission of a photon with energy 2E.

Mentions: SHG is a microscopy technique that is highly responsive to fibrillar collagen (Chen et al., 2012). Collagen-I is a triple helix, made up of three α-helical chains, with these individual helices self-assembling into fibrils and larger-scale fibres. The peptide bonds linking together amino acids in the chains have their own dipole which, when amplified along the helix length of collagen-I, gives the fibrillar structure a permanent dipole moment (Fig. 2A). The lack of centrosymmetry that necessarily accompanies this gives collagen-l the optical properties required for SHG. The coherent process of SHG absorbs two identical low energy photons and emits one high energy photon of double the energy of the incident photons (Fig. 2B). This can only occur in a non-centrosymmetric molecule such as collagen-I (Campagnola, 2011). SHG benefits from only using endogenous species to provide contrast in measurement, preventing artefacts from use of exogenous agents (Chen et al., 2012), as well as decreased photobleaching and phototoxicity (Campagnola, 2011). Additionally, SHG can be performed on tissue sections hundreds of microns thick, which prevents artefacts and errors created from the cutting process, required for standard histology procedures (Campagnola, 2011).Fig. 2.


Quantitative analysis of 3D extracellular matrix remodelling by pancreatic stellate cells.

Robinson BK, Cortes E, Rice AJ, Sarper M, Del Río Hernández A - Biol Open (2016)

Representation of SHG signal generation from collagen-I. (A) The peptide bonds of the collagen chains create a permanent dipole moment along the triple helix that allows second harmonic generation. (B) Jablonski diagram of SHG. Excitation with two photons with identical energy E, leads to a virtual energy state and emission of a photon with energy 2E.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4920190&req=5

BIO017632F2: Representation of SHG signal generation from collagen-I. (A) The peptide bonds of the collagen chains create a permanent dipole moment along the triple helix that allows second harmonic generation. (B) Jablonski diagram of SHG. Excitation with two photons with identical energy E, leads to a virtual energy state and emission of a photon with energy 2E.
Mentions: SHG is a microscopy technique that is highly responsive to fibrillar collagen (Chen et al., 2012). Collagen-I is a triple helix, made up of three α-helical chains, with these individual helices self-assembling into fibrils and larger-scale fibres. The peptide bonds linking together amino acids in the chains have their own dipole which, when amplified along the helix length of collagen-I, gives the fibrillar structure a permanent dipole moment (Fig. 2A). The lack of centrosymmetry that necessarily accompanies this gives collagen-l the optical properties required for SHG. The coherent process of SHG absorbs two identical low energy photons and emits one high energy photon of double the energy of the incident photons (Fig. 2B). This can only occur in a non-centrosymmetric molecule such as collagen-I (Campagnola, 2011). SHG benefits from only using endogenous species to provide contrast in measurement, preventing artefacts from use of exogenous agents (Chen et al., 2012), as well as decreased photobleaching and phototoxicity (Campagnola, 2011). Additionally, SHG can be performed on tissue sections hundreds of microns thick, which prevents artefacts and errors created from the cutting process, required for standard histology procedures (Campagnola, 2011).Fig. 2.

Bottom Line: We present an integrated methodology where cell-ECM interactions can be investigated in 3D environments via ECM remodelling.Pancreatic stellate cells (PSCs) are the key effectors of the stromal fibrosis associated to pancreatic cancer.We use PSCs to implement our methodology and demonstrate that PSC matrix remodelling capabilities depend on their contractile machinery and β1 integrin-mediated cell-ECM attachment.

View Article: PubMed Central - PubMed

Affiliation: Cellular and Molecular Biomechanics Laboratory, Department of Bioengineering, Faculty of Engineering, Imperial College London, South Kensington Campus, London SW7 2AZ, UK.

No MeSH data available.


Related in: MedlinePlus