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Disruption of a cystine transporter downregulates expression of genes involved in sulfur regulation and cellular respiration.

Simpkins JA, Rickel KE, Madeo M, Ahlers BA, Carlisle GB, Nelson HJ, Cardillo AL, Weber EA, Vitiello PF, Pearce DA, Vitiello SP - Biol Open (2016)

Bottom Line: Upon comparison of the transcriptomes of isogenic ERS1+ and ers1-Δ strains of S. cerevisiae by DNA microarray followed by targeted qPCR, sixteen genes were identified as being differentially expressed between the two genotypes.Genes that encode proteins functioning in sulfur regulation, cellular respiration, and general transport were enriched in our screen, demonstrating pleiotropic effects of ers1-Δ.These results give insight into yeast cystine regulation and the multiple, seemingly distal, pathways that involve proper cystine recycling.

View Article: PubMed Central - PubMed

Affiliation: Biology Department, Augustana University, Sioux Falls, SD, USA 57197.

No MeSH data available.


Related in: MedlinePlus

Genetic interactions with ERS1. Genes found to have differential expression in ers1-Δ were categorized into several functional groups, with energy and sulfur homeostasis as the two major groups. Most genes fell into more than one group.
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BIO017517F2: Genetic interactions with ERS1. Genes found to have differential expression in ers1-Δ were categorized into several functional groups, with energy and sulfur homeostasis as the two major groups. Most genes fell into more than one group.

Mentions: The genes identified in the screen could be categorized into several key processes: cellular respiration and redox-related, sulfur regulation, and the DAL5 subfamily of transporters (Fig. 2). Many of these genes are important in multiple overlapping cellular processes; for example, genes involved in sulfur regulation could also play a role in redox regulation. Genes involved in mating and sporulation and cell wall composition may be differentially expressed as a stress response, but can also be functionally categorized in the other groups.Fig. 2.


Disruption of a cystine transporter downregulates expression of genes involved in sulfur regulation and cellular respiration.

Simpkins JA, Rickel KE, Madeo M, Ahlers BA, Carlisle GB, Nelson HJ, Cardillo AL, Weber EA, Vitiello PF, Pearce DA, Vitiello SP - Biol Open (2016)

Genetic interactions with ERS1. Genes found to have differential expression in ers1-Δ were categorized into several functional groups, with energy and sulfur homeostasis as the two major groups. Most genes fell into more than one group.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4920189&req=5

BIO017517F2: Genetic interactions with ERS1. Genes found to have differential expression in ers1-Δ were categorized into several functional groups, with energy and sulfur homeostasis as the two major groups. Most genes fell into more than one group.
Mentions: The genes identified in the screen could be categorized into several key processes: cellular respiration and redox-related, sulfur regulation, and the DAL5 subfamily of transporters (Fig. 2). Many of these genes are important in multiple overlapping cellular processes; for example, genes involved in sulfur regulation could also play a role in redox regulation. Genes involved in mating and sporulation and cell wall composition may be differentially expressed as a stress response, but can also be functionally categorized in the other groups.Fig. 2.

Bottom Line: Upon comparison of the transcriptomes of isogenic ERS1+ and ers1-Δ strains of S. cerevisiae by DNA microarray followed by targeted qPCR, sixteen genes were identified as being differentially expressed between the two genotypes.Genes that encode proteins functioning in sulfur regulation, cellular respiration, and general transport were enriched in our screen, demonstrating pleiotropic effects of ers1-Δ.These results give insight into yeast cystine regulation and the multiple, seemingly distal, pathways that involve proper cystine recycling.

View Article: PubMed Central - PubMed

Affiliation: Biology Department, Augustana University, Sioux Falls, SD, USA 57197.

No MeSH data available.


Related in: MedlinePlus