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Amyloids assemble as part of recognizable structures during oogenesis in Xenopus.

Hayes MH, Weeks DL - Biol Open (2016)

Bottom Line: A hallmark of Alzheimer's, Huntington's and similar diseases is the assembly of proteins into amyloids rather than folding into their native state.There is an increasing appreciation that amyloids, under specific conditions, may be non-pathogenic.In the nucleus, we find particles associated with transcription by RNA polymerase I, II and III and RNA processing contain amyloids.

View Article: PubMed Central - PubMed

Affiliation: Molecular and Cellular Biology Program, Carver College of Medicine, University of Iowa, Iowa City, IA 52242, USA Medical Scientist Training Program, Carver College of Medicine, University of Iowa, Iowa City, IA 52242, USA.

No MeSH data available.


Related in: MedlinePlus

Isolated Xenopus nuclei (GVs) can be used for combinatorial identification of nuclear particles with thioflavin T and particle specific antibodies. (A) Manual removal of a GV from a stage VI oocyte. Scale bar: 500 µm. (B) Isolated GVs demonstrate amyloid containing particles seconds after thio-T staining. Scale bar: 200 µm. (C) Overlay of nucleolin immunofluorescence (red, D) and thio-T (green, E) staining of an isolated GV. Scale bar: 100 µm. (F) Overlay of images (G, green) stained with thio-T and (H, red) coilin immunofluorescence. A pearl particle is circled with a solid line, a histone locus body circled with a dashed line. Scale bar: 5 µm. (I) Overlay of panels (J, green) stained with thio-T and (K, red) SC35 immunofluorescence. Dashed lines indicate some thio-T-positive SC35-negative particles, dotted lines indicate a thio-T- and SC35-positive particle. The arrowhead points to a nucleolus in I-K. Scale bars: 5 µm.
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BIO017384F2: Isolated Xenopus nuclei (GVs) can be used for combinatorial identification of nuclear particles with thioflavin T and particle specific antibodies. (A) Manual removal of a GV from a stage VI oocyte. Scale bar: 500 µm. (B) Isolated GVs demonstrate amyloid containing particles seconds after thio-T staining. Scale bar: 200 µm. (C) Overlay of nucleolin immunofluorescence (red, D) and thio-T (green, E) staining of an isolated GV. Scale bar: 100 µm. (F) Overlay of images (G, green) stained with thio-T and (H, red) coilin immunofluorescence. A pearl particle is circled with a solid line, a histone locus body circled with a dashed line. Scale bar: 5 µm. (I) Overlay of panels (J, green) stained with thio-T and (K, red) SC35 immunofluorescence. Dashed lines indicate some thio-T-positive SC35-negative particles, dotted lines indicate a thio-T- and SC35-positive particle. The arrowhead points to a nucleolus in I-K. Scale bars: 5 µm.

Mentions: Due to their large size, Xenopus oocyte nuclei, known as germinal vesicles (GVs), are an ideal system to study sub-nuclear organelles. To identify and characterize the amyloid content of nuclear structures we analyzed unfixed GVs (Fig. 2A).Fig. 2.


Amyloids assemble as part of recognizable structures during oogenesis in Xenopus.

Hayes MH, Weeks DL - Biol Open (2016)

Isolated Xenopus nuclei (GVs) can be used for combinatorial identification of nuclear particles with thioflavin T and particle specific antibodies. (A) Manual removal of a GV from a stage VI oocyte. Scale bar: 500 µm. (B) Isolated GVs demonstrate amyloid containing particles seconds after thio-T staining. Scale bar: 200 µm. (C) Overlay of nucleolin immunofluorescence (red, D) and thio-T (green, E) staining of an isolated GV. Scale bar: 100 µm. (F) Overlay of images (G, green) stained with thio-T and (H, red) coilin immunofluorescence. A pearl particle is circled with a solid line, a histone locus body circled with a dashed line. Scale bar: 5 µm. (I) Overlay of panels (J, green) stained with thio-T and (K, red) SC35 immunofluorescence. Dashed lines indicate some thio-T-positive SC35-negative particles, dotted lines indicate a thio-T- and SC35-positive particle. The arrowhead points to a nucleolus in I-K. Scale bars: 5 µm.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4920187&req=5

BIO017384F2: Isolated Xenopus nuclei (GVs) can be used for combinatorial identification of nuclear particles with thioflavin T and particle specific antibodies. (A) Manual removal of a GV from a stage VI oocyte. Scale bar: 500 µm. (B) Isolated GVs demonstrate amyloid containing particles seconds after thio-T staining. Scale bar: 200 µm. (C) Overlay of nucleolin immunofluorescence (red, D) and thio-T (green, E) staining of an isolated GV. Scale bar: 100 µm. (F) Overlay of images (G, green) stained with thio-T and (H, red) coilin immunofluorescence. A pearl particle is circled with a solid line, a histone locus body circled with a dashed line. Scale bar: 5 µm. (I) Overlay of panels (J, green) stained with thio-T and (K, red) SC35 immunofluorescence. Dashed lines indicate some thio-T-positive SC35-negative particles, dotted lines indicate a thio-T- and SC35-positive particle. The arrowhead points to a nucleolus in I-K. Scale bars: 5 µm.
Mentions: Due to their large size, Xenopus oocyte nuclei, known as germinal vesicles (GVs), are an ideal system to study sub-nuclear organelles. To identify and characterize the amyloid content of nuclear structures we analyzed unfixed GVs (Fig. 2A).Fig. 2.

Bottom Line: A hallmark of Alzheimer's, Huntington's and similar diseases is the assembly of proteins into amyloids rather than folding into their native state.There is an increasing appreciation that amyloids, under specific conditions, may be non-pathogenic.In the nucleus, we find particles associated with transcription by RNA polymerase I, II and III and RNA processing contain amyloids.

View Article: PubMed Central - PubMed

Affiliation: Molecular and Cellular Biology Program, Carver College of Medicine, University of Iowa, Iowa City, IA 52242, USA Medical Scientist Training Program, Carver College of Medicine, University of Iowa, Iowa City, IA 52242, USA.

No MeSH data available.


Related in: MedlinePlus