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Zebrafish fin regeneration after cryoinjury-induced tissue damage.

Chassot B, Pury D, Jaźwińska A - Biol Open (2016)

Bottom Line: In contrast to the common transection model, the damaged part of the fin was spontaneously shed within two days after cryoinjury.Between two and seven days after cryoinjury, this reparative/proliferative phase was morphologically featured by displaced fragments of broken bones.Live imaging of epithelial and osteoblastic transgenic reporter lines revealed that the tissue-specific regenerative programmes were initiated after the clearance of damaged material.

View Article: PubMed Central - PubMed

Affiliation: Department of Biology, University of Fribourg, Chemin du Musée 10, Fribourg 1700, Switzerland.

No MeSH data available.


Related in: MedlinePlus

The blastemal marker msxB is upregulated in regenerating fins after cryoinjury. (A-D) In situ hybridization of longitudinal fin sections using msxB antisense probe (purple) at 3 dpa (A) and different time points after cryoinjury (B-D). Dashed line indicates the amputation plane. Bracket indicates the damaged tissue remaining in the cryoinjured stump. In the amputation model, msxB is upregulated in the mesenchyme of the outgrowth. After cryoinjury, msxB expression is induced below the damaged tissue. At 7 dpci (D), a normal blastema is formed, despite small amounts of distally remaining tissue debris. e, epidermis; m, mesenchyme. N=4. Scale bar=100 µm.
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BIO016865F7: The blastemal marker msxB is upregulated in regenerating fins after cryoinjury. (A-D) In situ hybridization of longitudinal fin sections using msxB antisense probe (purple) at 3 dpa (A) and different time points after cryoinjury (B-D). Dashed line indicates the amputation plane. Bracket indicates the damaged tissue remaining in the cryoinjured stump. In the amputation model, msxB is upregulated in the mesenchyme of the outgrowth. After cryoinjury, msxB expression is induced below the damaged tissue. At 7 dpci (D), a normal blastema is formed, despite small amounts of distally remaining tissue debris. e, epidermis; m, mesenchyme. N=4. Scale bar=100 µm.

Mentions: To determine whether cell proliferation was associated with upregulation of blastema genes, we analysed the expression of msxB, which is a well-established marker of the undifferentiated mesenchyme in the regenerative outgrowth in the fin amputation model (Fig. 7A) (Akimenko et al., 1995). We found that msxb was induced after cryoinjury in the proximal part of the stump below the partially damaged tissue at 3 and 5 dpci (Fig. 7B,C). These results suggest that the mesenchymal cells are activated to form the blastema, despite a massive demolition of the distal tissue. Interestingly, at 7 dpci, when the damaged area was nearly completely resolved, the msxB expression reproduced the normal pattern that resembled the blastema in the amputation model at 3 dpa (Fig. 7A,D). We concluded that after cryoinjury, cellular proliferation and blastema formation are resumed in a delayed fashion as compared to the amputation model.Fig. 7.


Zebrafish fin regeneration after cryoinjury-induced tissue damage.

Chassot B, Pury D, Jaźwińska A - Biol Open (2016)

The blastemal marker msxB is upregulated in regenerating fins after cryoinjury. (A-D) In situ hybridization of longitudinal fin sections using msxB antisense probe (purple) at 3 dpa (A) and different time points after cryoinjury (B-D). Dashed line indicates the amputation plane. Bracket indicates the damaged tissue remaining in the cryoinjured stump. In the amputation model, msxB is upregulated in the mesenchyme of the outgrowth. After cryoinjury, msxB expression is induced below the damaged tissue. At 7 dpci (D), a normal blastema is formed, despite small amounts of distally remaining tissue debris. e, epidermis; m, mesenchyme. N=4. Scale bar=100 µm.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4920184&req=5

BIO016865F7: The blastemal marker msxB is upregulated in regenerating fins after cryoinjury. (A-D) In situ hybridization of longitudinal fin sections using msxB antisense probe (purple) at 3 dpa (A) and different time points after cryoinjury (B-D). Dashed line indicates the amputation plane. Bracket indicates the damaged tissue remaining in the cryoinjured stump. In the amputation model, msxB is upregulated in the mesenchyme of the outgrowth. After cryoinjury, msxB expression is induced below the damaged tissue. At 7 dpci (D), a normal blastema is formed, despite small amounts of distally remaining tissue debris. e, epidermis; m, mesenchyme. N=4. Scale bar=100 µm.
Mentions: To determine whether cell proliferation was associated with upregulation of blastema genes, we analysed the expression of msxB, which is a well-established marker of the undifferentiated mesenchyme in the regenerative outgrowth in the fin amputation model (Fig. 7A) (Akimenko et al., 1995). We found that msxb was induced after cryoinjury in the proximal part of the stump below the partially damaged tissue at 3 and 5 dpci (Fig. 7B,C). These results suggest that the mesenchymal cells are activated to form the blastema, despite a massive demolition of the distal tissue. Interestingly, at 7 dpci, when the damaged area was nearly completely resolved, the msxB expression reproduced the normal pattern that resembled the blastema in the amputation model at 3 dpa (Fig. 7A,D). We concluded that after cryoinjury, cellular proliferation and blastema formation are resumed in a delayed fashion as compared to the amputation model.Fig. 7.

Bottom Line: In contrast to the common transection model, the damaged part of the fin was spontaneously shed within two days after cryoinjury.Between two and seven days after cryoinjury, this reparative/proliferative phase was morphologically featured by displaced fragments of broken bones.Live imaging of epithelial and osteoblastic transgenic reporter lines revealed that the tissue-specific regenerative programmes were initiated after the clearance of damaged material.

View Article: PubMed Central - PubMed

Affiliation: Department of Biology, University of Fribourg, Chemin du Musée 10, Fribourg 1700, Switzerland.

No MeSH data available.


Related in: MedlinePlus