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Zebrafish fin regeneration after cryoinjury-induced tissue damage.

Chassot B, Pury D, Jaźwińska A - Biol Open (2016)

Bottom Line: In contrast to the common transection model, the damaged part of the fin was spontaneously shed within two days after cryoinjury.Between two and seven days after cryoinjury, this reparative/proliferative phase was morphologically featured by displaced fragments of broken bones.Live imaging of epithelial and osteoblastic transgenic reporter lines revealed that the tissue-specific regenerative programmes were initiated after the clearance of damaged material.

View Article: PubMed Central - PubMed

Affiliation: Department of Biology, University of Fribourg, Chemin du Musée 10, Fribourg 1700, Switzerland.

No MeSH data available.


Related in: MedlinePlus

Enhanced cell proliferation and upregulation of tissue remodelling protein during regeneration of cryoinjured fins. (A-D) TUNEL labelling (green) of whole-mount fins at 3 dpa (A) and at different time points after cryoinjury (B-D). At 3 dpa (A), TUNEL staining is nearly absent. At 3 dpci (B), 5 dpci (C) and 7 dpci (D), tissue debris (dark structures in bright-field images) are associated with TUNEL-positive cells. (E-H) Immunodetection of BrdU (red) in whole-mount caudal fins at 3 dpa (E) and at different time points after cryoinjury (F-H). As compared to 3 dpa (E), BrdU-incorporation is lower in cryoinjured fins at 3 dpci (F), especially at the position of necrotic cells (dark regions in the bright-field). Cell proliferation becomes more abundant at 5 dpci (G) and 7 dpci (H). The dashed yellow line indicates the plane of amputation. The edge of the fin is indicated with a white dotted line. N=5. Scale bar=100 µm.
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BIO016865F6: Enhanced cell proliferation and upregulation of tissue remodelling protein during regeneration of cryoinjured fins. (A-D) TUNEL labelling (green) of whole-mount fins at 3 dpa (A) and at different time points after cryoinjury (B-D). At 3 dpa (A), TUNEL staining is nearly absent. At 3 dpci (B), 5 dpci (C) and 7 dpci (D), tissue debris (dark structures in bright-field images) are associated with TUNEL-positive cells. (E-H) Immunodetection of BrdU (red) in whole-mount caudal fins at 3 dpa (E) and at different time points after cryoinjury (F-H). As compared to 3 dpa (E), BrdU-incorporation is lower in cryoinjured fins at 3 dpci (F), especially at the position of necrotic cells (dark regions in the bright-field). Cell proliferation becomes more abundant at 5 dpci (G) and 7 dpci (H). The dashed yellow line indicates the plane of amputation. The edge of the fin is indicated with a white dotted line. N=5. Scale bar=100 µm.

Mentions: To determine whether the formation of the outgrowth is associated with apoptosis, we first performed a TUNEL assay on whole-mount fins. In fins after amputation, at 3 dpa, we observed only few scattered TUNEL-positive cells (Fig. 6A). However, after cryoininjury, at 3, 5 and 7 dpci, the margin of the regenerating stump displayed the remarkable presence of TUNEL-labelled cells (Fig. 6B-D). Thus, the transition from the reparative to the regenerative phase is associated with the continuous apoptotic elimination of cells in the partially damaged part of the stump.Fig. 6.


Zebrafish fin regeneration after cryoinjury-induced tissue damage.

Chassot B, Pury D, Jaźwińska A - Biol Open (2016)

Enhanced cell proliferation and upregulation of tissue remodelling protein during regeneration of cryoinjured fins. (A-D) TUNEL labelling (green) of whole-mount fins at 3 dpa (A) and at different time points after cryoinjury (B-D). At 3 dpa (A), TUNEL staining is nearly absent. At 3 dpci (B), 5 dpci (C) and 7 dpci (D), tissue debris (dark structures in bright-field images) are associated with TUNEL-positive cells. (E-H) Immunodetection of BrdU (red) in whole-mount caudal fins at 3 dpa (E) and at different time points after cryoinjury (F-H). As compared to 3 dpa (E), BrdU-incorporation is lower in cryoinjured fins at 3 dpci (F), especially at the position of necrotic cells (dark regions in the bright-field). Cell proliferation becomes more abundant at 5 dpci (G) and 7 dpci (H). The dashed yellow line indicates the plane of amputation. The edge of the fin is indicated with a white dotted line. N=5. Scale bar=100 µm.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4920184&req=5

BIO016865F6: Enhanced cell proliferation and upregulation of tissue remodelling protein during regeneration of cryoinjured fins. (A-D) TUNEL labelling (green) of whole-mount fins at 3 dpa (A) and at different time points after cryoinjury (B-D). At 3 dpa (A), TUNEL staining is nearly absent. At 3 dpci (B), 5 dpci (C) and 7 dpci (D), tissue debris (dark structures in bright-field images) are associated with TUNEL-positive cells. (E-H) Immunodetection of BrdU (red) in whole-mount caudal fins at 3 dpa (E) and at different time points after cryoinjury (F-H). As compared to 3 dpa (E), BrdU-incorporation is lower in cryoinjured fins at 3 dpci (F), especially at the position of necrotic cells (dark regions in the bright-field). Cell proliferation becomes more abundant at 5 dpci (G) and 7 dpci (H). The dashed yellow line indicates the plane of amputation. The edge of the fin is indicated with a white dotted line. N=5. Scale bar=100 µm.
Mentions: To determine whether the formation of the outgrowth is associated with apoptosis, we first performed a TUNEL assay on whole-mount fins. In fins after amputation, at 3 dpa, we observed only few scattered TUNEL-positive cells (Fig. 6A). However, after cryoininjury, at 3, 5 and 7 dpci, the margin of the regenerating stump displayed the remarkable presence of TUNEL-labelled cells (Fig. 6B-D). Thus, the transition from the reparative to the regenerative phase is associated with the continuous apoptotic elimination of cells in the partially damaged part of the stump.Fig. 6.

Bottom Line: In contrast to the common transection model, the damaged part of the fin was spontaneously shed within two days after cryoinjury.Between two and seven days after cryoinjury, this reparative/proliferative phase was morphologically featured by displaced fragments of broken bones.Live imaging of epithelial and osteoblastic transgenic reporter lines revealed that the tissue-specific regenerative programmes were initiated after the clearance of damaged material.

View Article: PubMed Central - PubMed

Affiliation: Department of Biology, University of Fribourg, Chemin du Musée 10, Fribourg 1700, Switzerland.

No MeSH data available.


Related in: MedlinePlus