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Zebrafish fin regeneration after cryoinjury-induced tissue damage.

Chassot B, Pury D, Jaźwińska A - Biol Open (2016)

Bottom Line: In contrast to the common transection model, the damaged part of the fin was spontaneously shed within two days after cryoinjury.Between two and seven days after cryoinjury, this reparative/proliferative phase was morphologically featured by displaced fragments of broken bones.Live imaging of epithelial and osteoblastic transgenic reporter lines revealed that the tissue-specific regenerative programmes were initiated after the clearance of damaged material.

View Article: PubMed Central - PubMed

Affiliation: Department of Biology, University of Fribourg, Chemin du Musée 10, Fribourg 1700, Switzerland.

No MeSH data available.


Related in: MedlinePlus

Tissue loss after cryoinjury is associated with massive apoptosis. (A-C) Whole-mount staining with DAPI (blue) and TUNEL (green) of the original fin (A), at 8 hpci (B) and at 48 hpci (C). (A′-C′) Magnifications of the framed areas of the upper images. (A,A′) Uninjured fins contain few apoptotic cells at the distal margin. (B,B′) Before sloughing of the cryoinjured fin part at 8 hpci, extensive apoptosis in the distal part of the extremity is observed. (C,C′) After truncation of the damaged fin part at 48 hpci, the margin of the remaining stump still contains apoptotic cells. N=4. Scale bar in A=100 µm.
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BIO016865F2: Tissue loss after cryoinjury is associated with massive apoptosis. (A-C) Whole-mount staining with DAPI (blue) and TUNEL (green) of the original fin (A), at 8 hpci (B) and at 48 hpci (C). (A′-C′) Magnifications of the framed areas of the upper images. (A,A′) Uninjured fins contain few apoptotic cells at the distal margin. (B,B′) Before sloughing of the cryoinjured fin part at 8 hpci, extensive apoptosis in the distal part of the extremity is observed. (C,C′) After truncation of the damaged fin part at 48 hpci, the margin of the remaining stump still contains apoptotic cells. N=4. Scale bar in A=100 µm.

Mentions: Live imaging of the fins at 12 hpci revealed that the tissue loss was initiated at the distal end of the fin, which had not been covered with ice crystals during the freezing procedure. To understand the cellular causes underlying tissue rupture at this position, we analysed cell apoptosis using the terminal deoxynucleotidyl transferase digoxigenin-UTP nick end-labelling (TUNEL) assay. In uninjured control fins, only a few TUNEL-positive cells were detected in the appendage (Fig. 2A,A′). At 8 hpci, numerous apoptotic cells appeared in the distal portion of the fin (Fig. 2B,B′). We concluded that the entire portion of the fin above the cryoinjury plane has been severely affected by cryoinjury, which explains the complete detachment of this tissue within two days. After truncation of the apoptotic fin part at 48 hpci, TUNEL-positive cells were still detected in the remaining stump, indicating a partial destruction of the tissue (Fig. 2C,C′). The level of damage was, however, sufficient for maintaining the integrity of the distorted stump with the rest of the body.Fig. 2.


Zebrafish fin regeneration after cryoinjury-induced tissue damage.

Chassot B, Pury D, Jaźwińska A - Biol Open (2016)

Tissue loss after cryoinjury is associated with massive apoptosis. (A-C) Whole-mount staining with DAPI (blue) and TUNEL (green) of the original fin (A), at 8 hpci (B) and at 48 hpci (C). (A′-C′) Magnifications of the framed areas of the upper images. (A,A′) Uninjured fins contain few apoptotic cells at the distal margin. (B,B′) Before sloughing of the cryoinjured fin part at 8 hpci, extensive apoptosis in the distal part of the extremity is observed. (C,C′) After truncation of the damaged fin part at 48 hpci, the margin of the remaining stump still contains apoptotic cells. N=4. Scale bar in A=100 µm.
© Copyright Policy - open-access
Related In: Results  -  Collection

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BIO016865F2: Tissue loss after cryoinjury is associated with massive apoptosis. (A-C) Whole-mount staining with DAPI (blue) and TUNEL (green) of the original fin (A), at 8 hpci (B) and at 48 hpci (C). (A′-C′) Magnifications of the framed areas of the upper images. (A,A′) Uninjured fins contain few apoptotic cells at the distal margin. (B,B′) Before sloughing of the cryoinjured fin part at 8 hpci, extensive apoptosis in the distal part of the extremity is observed. (C,C′) After truncation of the damaged fin part at 48 hpci, the margin of the remaining stump still contains apoptotic cells. N=4. Scale bar in A=100 µm.
Mentions: Live imaging of the fins at 12 hpci revealed that the tissue loss was initiated at the distal end of the fin, which had not been covered with ice crystals during the freezing procedure. To understand the cellular causes underlying tissue rupture at this position, we analysed cell apoptosis using the terminal deoxynucleotidyl transferase digoxigenin-UTP nick end-labelling (TUNEL) assay. In uninjured control fins, only a few TUNEL-positive cells were detected in the appendage (Fig. 2A,A′). At 8 hpci, numerous apoptotic cells appeared in the distal portion of the fin (Fig. 2B,B′). We concluded that the entire portion of the fin above the cryoinjury plane has been severely affected by cryoinjury, which explains the complete detachment of this tissue within two days. After truncation of the apoptotic fin part at 48 hpci, TUNEL-positive cells were still detected in the remaining stump, indicating a partial destruction of the tissue (Fig. 2C,C′). The level of damage was, however, sufficient for maintaining the integrity of the distorted stump with the rest of the body.Fig. 2.

Bottom Line: In contrast to the common transection model, the damaged part of the fin was spontaneously shed within two days after cryoinjury.Between two and seven days after cryoinjury, this reparative/proliferative phase was morphologically featured by displaced fragments of broken bones.Live imaging of epithelial and osteoblastic transgenic reporter lines revealed that the tissue-specific regenerative programmes were initiated after the clearance of damaged material.

View Article: PubMed Central - PubMed

Affiliation: Department of Biology, University of Fribourg, Chemin du Musée 10, Fribourg 1700, Switzerland.

No MeSH data available.


Related in: MedlinePlus