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Tissue- and stage-specific Wnt target gene expression is controlled subsequent to β-catenin recruitment to cis-regulatory modules.

Nakamura Y, de Paiva Alves E, Veenstra GJ, Hoppler S - Development (2016)

Bottom Line: Here, a direct comparison of genome-wide occupancy of β-catenin with a stage-matched Wnt-regulated transcriptome reveals that only a subset of β-catenin-bound genomic loci are transcriptionally regulated by Wnt signalling.The transcriptional response to Wnt signalling depends on additional mechanisms, such as BMP or FGF signalling for the particular genes we investigated, which do not influence β-catenin recruitment.Chromatin association of β-catenin, even to functional Wnt-response elements, can no longer be considered a proxy for identifying transcriptionally Wnt-regulated genes.

View Article: PubMed Central - PubMed

Affiliation: Institute of Medical Sciences, Foresterhill Health Campus, University of Aberdeen, Aberdeen AB25 2ZD, UK.

No MeSH data available.


Related in: MedlinePlus

BMP or FGF signalling is required for wnt8a target gene expression but not for β-catenin recruitment. (A) BMP signalling is required for context-specific transcriptional regulation by Wnt8a signalling, but only of some wnt8a target genes. Two- to four-cell stage embryos were injected with BMP antagonist noggin (nog) mRNA. CSKA-wnt8a DNA was injected additionally to reinstate Wnt8a expression (as endogenous wnt8a expression is itself regulated by BMP signalling). Expression was analysed by RT-qPCR at the early gastrula stage. When BMP signalling is blocked, expression of BMP-dependent genes remains reduced even when Wnt8a expression is reinstated. (B) FGF signalling is required for context-specific transcriptional regulation by Wnt8a signalling, but only of some wnt8a target genes. Embryos were treated with the FGFR inhibitor SU5402 from the 1000/2000-cell stage through the early gastrula and injected where indicated with CSKA-wnt8a DNA at the two- to four-cell stages (to reinstate Wnt8a expression, as endogenous wnt8a expression is itself regulated by FGF signalling). When FGF signalling is inhibited, expression of FGF-dependent genes remains reduced, even when Wnt8a expression is reinstated. (C) wnt8a targets can therefore be classified into BMP-dependent or FGF-dependent genes. Note that some genes belong to both groups and others are neither BMP nor FGF dependent. (D,E) In situ hybridisation shows expression of msx1 (D) and hoxd1 (E) in sagittal sections and lateral views (insets) of control uninjected and experimentally manipulated embryos as indicated (dorsal to the right). (F) BMP signalling is not required for wnt8a-regulated β-catenin recruitment to BMP-dependent wnt8a target gene loci. Embryos were treated as in A and analysed by β-catenin ChIP-qPCR at the early gastrula stage. (G) FGF signalling is not essential for wnt8a-regulated β-catenin recruitment to FGF-dependent wnt8a target gene loci. Embryos were treated as in B and analysed by β-catenin ChIP-qPCR at the early gastrula stage. Uninjected, untreated embryos were used as controls in A,B,D-G. *P<0.1, **P<0.05; two-tailed Student's t-test. Error bars represent s.d. of four biological replicates (A,B) or s.e.m. of three biological replicates (F,G). Note that wnt8a gene expression itself was decreased by BMP or FGF pathway inhibition (wnt8a blue bars in A,B) but restored by co-injection of CSKA-wnt8a DNA (wnt8a orange bars in A,B) compared with controls (wnt8a yellow bars in A,B), and that higher wnt8a expression levels with CSKA-wnt8a DNA (wnt8a green bars in A,B) reflect both expression of endogenous wnt8a and expression from CSKA-wnt8a DNA, resulting in upregulation of several wnt8a target genes (in A,B).
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DEV131664F5: BMP or FGF signalling is required for wnt8a target gene expression but not for β-catenin recruitment. (A) BMP signalling is required for context-specific transcriptional regulation by Wnt8a signalling, but only of some wnt8a target genes. Two- to four-cell stage embryos were injected with BMP antagonist noggin (nog) mRNA. CSKA-wnt8a DNA was injected additionally to reinstate Wnt8a expression (as endogenous wnt8a expression is itself regulated by BMP signalling). Expression was analysed by RT-qPCR at the early gastrula stage. When BMP signalling is blocked, expression of BMP-dependent genes remains reduced even when Wnt8a expression is reinstated. (B) FGF signalling is required for context-specific transcriptional regulation by Wnt8a signalling, but only of some wnt8a target genes. Embryos were treated with the FGFR inhibitor SU5402 from the 1000/2000-cell stage through the early gastrula and injected where indicated with CSKA-wnt8a DNA at the two- to four-cell stages (to reinstate Wnt8a expression, as endogenous wnt8a expression is itself regulated by FGF signalling). When FGF signalling is inhibited, expression of FGF-dependent genes remains reduced, even when Wnt8a expression is reinstated. (C) wnt8a targets can therefore be classified into BMP-dependent or FGF-dependent genes. Note that some genes belong to both groups and others are neither BMP nor FGF dependent. (D,E) In situ hybridisation shows expression of msx1 (D) and hoxd1 (E) in sagittal sections and lateral views (insets) of control uninjected and experimentally manipulated embryos as indicated (dorsal to the right). (F) BMP signalling is not required for wnt8a-regulated β-catenin recruitment to BMP-dependent wnt8a target gene loci. Embryos were treated as in A and analysed by β-catenin ChIP-qPCR at the early gastrula stage. (G) FGF signalling is not essential for wnt8a-regulated β-catenin recruitment to FGF-dependent wnt8a target gene loci. Embryos were treated as in B and analysed by β-catenin ChIP-qPCR at the early gastrula stage. Uninjected, untreated embryos were used as controls in A,B,D-G. *P<0.1, **P<0.05; two-tailed Student's t-test. Error bars represent s.d. of four biological replicates (A,B) or s.e.m. of three biological replicates (F,G). Note that wnt8a gene expression itself was decreased by BMP or FGF pathway inhibition (wnt8a blue bars in A,B) but restored by co-injection of CSKA-wnt8a DNA (wnt8a orange bars in A,B) compared with controls (wnt8a yellow bars in A,B), and that higher wnt8a expression levels with CSKA-wnt8a DNA (wnt8a green bars in A,B) reflect both expression of endogenous wnt8a and expression from CSKA-wnt8a DNA, resulting in upregulation of several wnt8a target genes (in A,B).

Mentions: Beyond the expected TCF/LEF motifs, de novo motif discovery among wnt8a target genes failed to identify further shared enriched motifs. We therefore sought to test earlier proposed hypotheses that combinatorial signalling underlies the context-specific expression of wnt8a-regulated genes. It has previously been suggested that, among our wnt8a-regulated genes, ventx1.2 is co-regulated by BMP signalling (e.g. Hoppler and Moon, 1998). To investigate whether co-regulation by Wnt and BMP signalling represents a shared mechanism for regulating context-specific expression of wnt8a targets (reviewed by Itasaki and Hoppler, 2010), we examined the requirement of BMP signalling for wnt8a target gene regulation by blocking the BMP pathway while maintaining constant levels of Wnt8a signalling. We found that the expression of another four genes, in addition to ventx1.2, is dependent on BMP signalling (Fig. 5A), but, importantly, not that of all 13 genes in the shortlist. Thus, although decisive for context-specific expression of some genes in this tissue, BMP signalling is not an indispensable element of any general mechanism for context-specific regulation of wnt8a target genes.Fig. 5.


Tissue- and stage-specific Wnt target gene expression is controlled subsequent to β-catenin recruitment to cis-regulatory modules.

Nakamura Y, de Paiva Alves E, Veenstra GJ, Hoppler S - Development (2016)

BMP or FGF signalling is required for wnt8a target gene expression but not for β-catenin recruitment. (A) BMP signalling is required for context-specific transcriptional regulation by Wnt8a signalling, but only of some wnt8a target genes. Two- to four-cell stage embryos were injected with BMP antagonist noggin (nog) mRNA. CSKA-wnt8a DNA was injected additionally to reinstate Wnt8a expression (as endogenous wnt8a expression is itself regulated by BMP signalling). Expression was analysed by RT-qPCR at the early gastrula stage. When BMP signalling is blocked, expression of BMP-dependent genes remains reduced even when Wnt8a expression is reinstated. (B) FGF signalling is required for context-specific transcriptional regulation by Wnt8a signalling, but only of some wnt8a target genes. Embryos were treated with the FGFR inhibitor SU5402 from the 1000/2000-cell stage through the early gastrula and injected where indicated with CSKA-wnt8a DNA at the two- to four-cell stages (to reinstate Wnt8a expression, as endogenous wnt8a expression is itself regulated by FGF signalling). When FGF signalling is inhibited, expression of FGF-dependent genes remains reduced, even when Wnt8a expression is reinstated. (C) wnt8a targets can therefore be classified into BMP-dependent or FGF-dependent genes. Note that some genes belong to both groups and others are neither BMP nor FGF dependent. (D,E) In situ hybridisation shows expression of msx1 (D) and hoxd1 (E) in sagittal sections and lateral views (insets) of control uninjected and experimentally manipulated embryos as indicated (dorsal to the right). (F) BMP signalling is not required for wnt8a-regulated β-catenin recruitment to BMP-dependent wnt8a target gene loci. Embryos were treated as in A and analysed by β-catenin ChIP-qPCR at the early gastrula stage. (G) FGF signalling is not essential for wnt8a-regulated β-catenin recruitment to FGF-dependent wnt8a target gene loci. Embryos were treated as in B and analysed by β-catenin ChIP-qPCR at the early gastrula stage. Uninjected, untreated embryos were used as controls in A,B,D-G. *P<0.1, **P<0.05; two-tailed Student's t-test. Error bars represent s.d. of four biological replicates (A,B) or s.e.m. of three biological replicates (F,G). Note that wnt8a gene expression itself was decreased by BMP or FGF pathway inhibition (wnt8a blue bars in A,B) but restored by co-injection of CSKA-wnt8a DNA (wnt8a orange bars in A,B) compared with controls (wnt8a yellow bars in A,B), and that higher wnt8a expression levels with CSKA-wnt8a DNA (wnt8a green bars in A,B) reflect both expression of endogenous wnt8a and expression from CSKA-wnt8a DNA, resulting in upregulation of several wnt8a target genes (in A,B).
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DEV131664F5: BMP or FGF signalling is required for wnt8a target gene expression but not for β-catenin recruitment. (A) BMP signalling is required for context-specific transcriptional regulation by Wnt8a signalling, but only of some wnt8a target genes. Two- to four-cell stage embryos were injected with BMP antagonist noggin (nog) mRNA. CSKA-wnt8a DNA was injected additionally to reinstate Wnt8a expression (as endogenous wnt8a expression is itself regulated by BMP signalling). Expression was analysed by RT-qPCR at the early gastrula stage. When BMP signalling is blocked, expression of BMP-dependent genes remains reduced even when Wnt8a expression is reinstated. (B) FGF signalling is required for context-specific transcriptional regulation by Wnt8a signalling, but only of some wnt8a target genes. Embryos were treated with the FGFR inhibitor SU5402 from the 1000/2000-cell stage through the early gastrula and injected where indicated with CSKA-wnt8a DNA at the two- to four-cell stages (to reinstate Wnt8a expression, as endogenous wnt8a expression is itself regulated by FGF signalling). When FGF signalling is inhibited, expression of FGF-dependent genes remains reduced, even when Wnt8a expression is reinstated. (C) wnt8a targets can therefore be classified into BMP-dependent or FGF-dependent genes. Note that some genes belong to both groups and others are neither BMP nor FGF dependent. (D,E) In situ hybridisation shows expression of msx1 (D) and hoxd1 (E) in sagittal sections and lateral views (insets) of control uninjected and experimentally manipulated embryos as indicated (dorsal to the right). (F) BMP signalling is not required for wnt8a-regulated β-catenin recruitment to BMP-dependent wnt8a target gene loci. Embryos were treated as in A and analysed by β-catenin ChIP-qPCR at the early gastrula stage. (G) FGF signalling is not essential for wnt8a-regulated β-catenin recruitment to FGF-dependent wnt8a target gene loci. Embryos were treated as in B and analysed by β-catenin ChIP-qPCR at the early gastrula stage. Uninjected, untreated embryos were used as controls in A,B,D-G. *P<0.1, **P<0.05; two-tailed Student's t-test. Error bars represent s.d. of four biological replicates (A,B) or s.e.m. of three biological replicates (F,G). Note that wnt8a gene expression itself was decreased by BMP or FGF pathway inhibition (wnt8a blue bars in A,B) but restored by co-injection of CSKA-wnt8a DNA (wnt8a orange bars in A,B) compared with controls (wnt8a yellow bars in A,B), and that higher wnt8a expression levels with CSKA-wnt8a DNA (wnt8a green bars in A,B) reflect both expression of endogenous wnt8a and expression from CSKA-wnt8a DNA, resulting in upregulation of several wnt8a target genes (in A,B).
Mentions: Beyond the expected TCF/LEF motifs, de novo motif discovery among wnt8a target genes failed to identify further shared enriched motifs. We therefore sought to test earlier proposed hypotheses that combinatorial signalling underlies the context-specific expression of wnt8a-regulated genes. It has previously been suggested that, among our wnt8a-regulated genes, ventx1.2 is co-regulated by BMP signalling (e.g. Hoppler and Moon, 1998). To investigate whether co-regulation by Wnt and BMP signalling represents a shared mechanism for regulating context-specific expression of wnt8a targets (reviewed by Itasaki and Hoppler, 2010), we examined the requirement of BMP signalling for wnt8a target gene regulation by blocking the BMP pathway while maintaining constant levels of Wnt8a signalling. We found that the expression of another four genes, in addition to ventx1.2, is dependent on BMP signalling (Fig. 5A), but, importantly, not that of all 13 genes in the shortlist. Thus, although decisive for context-specific expression of some genes in this tissue, BMP signalling is not an indispensable element of any general mechanism for context-specific regulation of wnt8a target genes.Fig. 5.

Bottom Line: Here, a direct comparison of genome-wide occupancy of β-catenin with a stage-matched Wnt-regulated transcriptome reveals that only a subset of β-catenin-bound genomic loci are transcriptionally regulated by Wnt signalling.The transcriptional response to Wnt signalling depends on additional mechanisms, such as BMP or FGF signalling for the particular genes we investigated, which do not influence β-catenin recruitment.Chromatin association of β-catenin, even to functional Wnt-response elements, can no longer be considered a proxy for identifying transcriptionally Wnt-regulated genes.

View Article: PubMed Central - PubMed

Affiliation: Institute of Medical Sciences, Foresterhill Health Campus, University of Aberdeen, Aberdeen AB25 2ZD, UK.

No MeSH data available.


Related in: MedlinePlus