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Exposure to Sevoflurane Affects the Development of Parvalbumin Interneurons in the Main Olfactory Bulb in Mice.

Yang J, Chen J, Cai G, Lu R, Sun T, Luo T, Wu S, Ling S - Front Neuroanat (2016)

Bottom Line: Sevoflurane is widely used in adult and pediatric patients during clinical surgeries.This study detected the effect of sevoflurane exposure on the development of calcium-binding proteins-expressing interneurons in the main olfactory bulb (MOB).We exposed neonatal mice to 2% sevoflurane at two different developmental time points and found that exposing mice to sevoflurane at postnatal day (PD) 7 significantly decreased the expression of GAD67 and parvalbumin (PV) in the olfactory bulb (OB) but did not alter the expression of calretinin (CR) or calbindin D28k (CB).

View Article: PubMed Central - PubMed

Affiliation: Institute of Neuroscience and Anatomy, School of Medicine, Zhejiang University Hangzhou, China.

ABSTRACT
Sevoflurane is widely used in adult and pediatric patients during clinical surgeries. Although studies have shown that exposure to sevoflurane impairs solfactory memory after an operation, the neuropathological changes underlying this effect are not clear. This study detected the effect of sevoflurane exposure on the development of calcium-binding proteins-expressing interneurons in the main olfactory bulb (MOB). We exposed neonatal mice to 2% sevoflurane at two different developmental time points and found that exposing mice to sevoflurane at postnatal day (PD) 7 significantly decreased the expression of GAD67 and parvalbumin (PV) in the olfactory bulb (OB) but did not alter the expression of calretinin (CR) or calbindin D28k (CB). The number and dendritic morphology of PV-expressing interneurons in the MOB were impaired by exposure to sevoflurane at PD7. However, exposure to sevoflurane at PD10 had no effect on calcium-binding protein expression or the number and dendritic morphology of PV-expressing interneurons in the MOB. These results suggest that exposing neonatal mice to sevoflurane during a critical period of olfactory development affects the development of PV-expressing interneurons in the MOB.

No MeSH data available.


Related in: MedlinePlus

The effect of exposure to sevoflurane at PD10 on the number and dendritic morphology of PV interneurons. Compared to the control group (A–C), the number and dendritic morphologies of PV interneurons were not impaired by exposure to sevoflurane at PD10 (D–F). (C1,C2,F1,F2) High magnification images of PV interneurons in the MOB. (G) Quantification analysis of the number of PV-ir neurons in different layers of MOB. Scale bars = 200 μm in (D) (applies to A,D), 100 μm in (E) (applies to B,E), 50 μm in (F) (applies to C,F), and 5 μm in (F2) (applies to C1,C2,F1,F2). Values are expressed as the Mean ± SD.
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Figure 6: The effect of exposure to sevoflurane at PD10 on the number and dendritic morphology of PV interneurons. Compared to the control group (A–C), the number and dendritic morphologies of PV interneurons were not impaired by exposure to sevoflurane at PD10 (D–F). (C1,C2,F1,F2) High magnification images of PV interneurons in the MOB. (G) Quantification analysis of the number of PV-ir neurons in different layers of MOB. Scale bars = 200 μm in (D) (applies to A,D), 100 μm in (E) (applies to B,E), 50 μm in (F) (applies to C,F), and 5 μm in (F2) (applies to C1,C2,F1,F2). Values are expressed as the Mean ± SD.

Mentions: To further confirm the effects of sevoflurane exposure at PD10 on PV-expressing interneurons at the cellular level, immunofluorescence staining was used to detect the numbers and morphologies of PV-ir interneurons in the MOB of control and sevoflurane-treated mice at PD42. No difference was observed in the number of PV-ir interneurons not only in EPL but also in the ML and IPL of the MOB between control mice and sevoflurane-treated mice (Figures 6A,B,D,E,G, P > 0.05). The dendrites of the PV-ir neurons in the MOB were clear and ramulose in both the control mice and the sevoflurane-exposed mice (Figures 6C–C2,F–F2). This indicated that sevoflurane exposure at PD10 had no effect on the PV-expressing interneurons of the MOB.


Exposure to Sevoflurane Affects the Development of Parvalbumin Interneurons in the Main Olfactory Bulb in Mice.

Yang J, Chen J, Cai G, Lu R, Sun T, Luo T, Wu S, Ling S - Front Neuroanat (2016)

The effect of exposure to sevoflurane at PD10 on the number and dendritic morphology of PV interneurons. Compared to the control group (A–C), the number and dendritic morphologies of PV interneurons were not impaired by exposure to sevoflurane at PD10 (D–F). (C1,C2,F1,F2) High magnification images of PV interneurons in the MOB. (G) Quantification analysis of the number of PV-ir neurons in different layers of MOB. Scale bars = 200 μm in (D) (applies to A,D), 100 μm in (E) (applies to B,E), 50 μm in (F) (applies to C,F), and 5 μm in (F2) (applies to C1,C2,F1,F2). Values are expressed as the Mean ± SD.
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Figure 6: The effect of exposure to sevoflurane at PD10 on the number and dendritic morphology of PV interneurons. Compared to the control group (A–C), the number and dendritic morphologies of PV interneurons were not impaired by exposure to sevoflurane at PD10 (D–F). (C1,C2,F1,F2) High magnification images of PV interneurons in the MOB. (G) Quantification analysis of the number of PV-ir neurons in different layers of MOB. Scale bars = 200 μm in (D) (applies to A,D), 100 μm in (E) (applies to B,E), 50 μm in (F) (applies to C,F), and 5 μm in (F2) (applies to C1,C2,F1,F2). Values are expressed as the Mean ± SD.
Mentions: To further confirm the effects of sevoflurane exposure at PD10 on PV-expressing interneurons at the cellular level, immunofluorescence staining was used to detect the numbers and morphologies of PV-ir interneurons in the MOB of control and sevoflurane-treated mice at PD42. No difference was observed in the number of PV-ir interneurons not only in EPL but also in the ML and IPL of the MOB between control mice and sevoflurane-treated mice (Figures 6A,B,D,E,G, P > 0.05). The dendrites of the PV-ir neurons in the MOB were clear and ramulose in both the control mice and the sevoflurane-exposed mice (Figures 6C–C2,F–F2). This indicated that sevoflurane exposure at PD10 had no effect on the PV-expressing interneurons of the MOB.

Bottom Line: Sevoflurane is widely used in adult and pediatric patients during clinical surgeries.This study detected the effect of sevoflurane exposure on the development of calcium-binding proteins-expressing interneurons in the main olfactory bulb (MOB).We exposed neonatal mice to 2% sevoflurane at two different developmental time points and found that exposing mice to sevoflurane at postnatal day (PD) 7 significantly decreased the expression of GAD67 and parvalbumin (PV) in the olfactory bulb (OB) but did not alter the expression of calretinin (CR) or calbindin D28k (CB).

View Article: PubMed Central - PubMed

Affiliation: Institute of Neuroscience and Anatomy, School of Medicine, Zhejiang University Hangzhou, China.

ABSTRACT
Sevoflurane is widely used in adult and pediatric patients during clinical surgeries. Although studies have shown that exposure to sevoflurane impairs solfactory memory after an operation, the neuropathological changes underlying this effect are not clear. This study detected the effect of sevoflurane exposure on the development of calcium-binding proteins-expressing interneurons in the main olfactory bulb (MOB). We exposed neonatal mice to 2% sevoflurane at two different developmental time points and found that exposing mice to sevoflurane at postnatal day (PD) 7 significantly decreased the expression of GAD67 and parvalbumin (PV) in the olfactory bulb (OB) but did not alter the expression of calretinin (CR) or calbindin D28k (CB). The number and dendritic morphology of PV-expressing interneurons in the MOB were impaired by exposure to sevoflurane at PD7. However, exposure to sevoflurane at PD10 had no effect on calcium-binding protein expression or the number and dendritic morphology of PV-expressing interneurons in the MOB. These results suggest that exposing neonatal mice to sevoflurane during a critical period of olfactory development affects the development of PV-expressing interneurons in the MOB.

No MeSH data available.


Related in: MedlinePlus