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Exposure to Sevoflurane Affects the Development of Parvalbumin Interneurons in the Main Olfactory Bulb in Mice.

Yang J, Chen J, Cai G, Lu R, Sun T, Luo T, Wu S, Ling S - Front Neuroanat (2016)

Bottom Line: Sevoflurane is widely used in adult and pediatric patients during clinical surgeries.This study detected the effect of sevoflurane exposure on the development of calcium-binding proteins-expressing interneurons in the main olfactory bulb (MOB).We exposed neonatal mice to 2% sevoflurane at two different developmental time points and found that exposing mice to sevoflurane at postnatal day (PD) 7 significantly decreased the expression of GAD67 and parvalbumin (PV) in the olfactory bulb (OB) but did not alter the expression of calretinin (CR) or calbindin D28k (CB).

View Article: PubMed Central - PubMed

Affiliation: Institute of Neuroscience and Anatomy, School of Medicine, Zhejiang University Hangzhou, China.

ABSTRACT
Sevoflurane is widely used in adult and pediatric patients during clinical surgeries. Although studies have shown that exposure to sevoflurane impairs solfactory memory after an operation, the neuropathological changes underlying this effect are not clear. This study detected the effect of sevoflurane exposure on the development of calcium-binding proteins-expressing interneurons in the main olfactory bulb (MOB). We exposed neonatal mice to 2% sevoflurane at two different developmental time points and found that exposing mice to sevoflurane at postnatal day (PD) 7 significantly decreased the expression of GAD67 and parvalbumin (PV) in the olfactory bulb (OB) but did not alter the expression of calretinin (CR) or calbindin D28k (CB). The number and dendritic morphology of PV-expressing interneurons in the MOB were impaired by exposure to sevoflurane at PD7. However, exposure to sevoflurane at PD10 had no effect on calcium-binding protein expression or the number and dendritic morphology of PV-expressing interneurons in the MOB. These results suggest that exposing neonatal mice to sevoflurane during a critical period of olfactory development affects the development of PV-expressing interneurons in the MOB.

No MeSH data available.


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The effect of exposure to sevoflurane at PD7 on the dendritic architecture of calcium-binding protein-expressing neurons in the MOB. Compared to the control group (A,B), the dendritic morphologies of PV interneurons were impaired by exposure to sevoflurane at PD7 (C,D). (B1,B2,D1,D2) High magnification images of PV interneurons in the MOB. Compared to the control group (E,F), the dendritic morphologies of CR interneurons were not impaired by exposure to sevoflurane at PD7 (G,H). (F1,F2,H1,H2) High magnification images of CR interneurons in the MOB. Compared to the control group (I,J), the dendritic morphologies of CB interneurons were not impaired by exposure to sevoflurane at PD7 (K,L). (J1,J2,L1,L2) High magnification images of CR interneurons in the MOB. Scale bars = 100 μm in (K) (applies to A,C,E,G,I,K), 50 μm in (L) (applies to B,D,F,H,J,L), and 5 μm in (L2) (applies to B1,B2,D1,D2,F1,F2,H1,H2,J1,J2,L1,L2).
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Figure 4: The effect of exposure to sevoflurane at PD7 on the dendritic architecture of calcium-binding protein-expressing neurons in the MOB. Compared to the control group (A,B), the dendritic morphologies of PV interneurons were impaired by exposure to sevoflurane at PD7 (C,D). (B1,B2,D1,D2) High magnification images of PV interneurons in the MOB. Compared to the control group (E,F), the dendritic morphologies of CR interneurons were not impaired by exposure to sevoflurane at PD7 (G,H). (F1,F2,H1,H2) High magnification images of CR interneurons in the MOB. Compared to the control group (I,J), the dendritic morphologies of CB interneurons were not impaired by exposure to sevoflurane at PD7 (K,L). (J1,J2,L1,L2) High magnification images of CR interneurons in the MOB. Scale bars = 100 μm in (K) (applies to A,C,E,G,I,K), 50 μm in (L) (applies to B,D,F,H,J,L), and 5 μm in (L2) (applies to B1,B2,D1,D2,F1,F2,H1,H2,J1,J2,L1,L2).

Mentions: To further evaluate the effect of sevoflurane on neuronal morphologies, immunofluorescence staining was used to display the dendritic arbor architecture of these interneurons in control and sevoflurane-exposed mice at PD42. The results showed that the PV-ir interneurons in the MOB in control mice were multipolar neurons with extensive and clear dendrite complexities with visible secondary and tertiary dendrites (Figures 4A–B2). In the sevoflurane-treated mice, the dendritic branches of PV-ir interneurons were significantly decreased, and some of these neurons had no dendrites (Figures 4D–D2). Unlike in the PV-expressing neurons, the dendrites of the CR-ir and CB-ir interneurons were not affected by sevoflurane exposure at PD7 (Figures 4E–L).


Exposure to Sevoflurane Affects the Development of Parvalbumin Interneurons in the Main Olfactory Bulb in Mice.

Yang J, Chen J, Cai G, Lu R, Sun T, Luo T, Wu S, Ling S - Front Neuroanat (2016)

The effect of exposure to sevoflurane at PD7 on the dendritic architecture of calcium-binding protein-expressing neurons in the MOB. Compared to the control group (A,B), the dendritic morphologies of PV interneurons were impaired by exposure to sevoflurane at PD7 (C,D). (B1,B2,D1,D2) High magnification images of PV interneurons in the MOB. Compared to the control group (E,F), the dendritic morphologies of CR interneurons were not impaired by exposure to sevoflurane at PD7 (G,H). (F1,F2,H1,H2) High magnification images of CR interneurons in the MOB. Compared to the control group (I,J), the dendritic morphologies of CB interneurons were not impaired by exposure to sevoflurane at PD7 (K,L). (J1,J2,L1,L2) High magnification images of CR interneurons in the MOB. Scale bars = 100 μm in (K) (applies to A,C,E,G,I,K), 50 μm in (L) (applies to B,D,F,H,J,L), and 5 μm in (L2) (applies to B1,B2,D1,D2,F1,F2,H1,H2,J1,J2,L1,L2).
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Figure 4: The effect of exposure to sevoflurane at PD7 on the dendritic architecture of calcium-binding protein-expressing neurons in the MOB. Compared to the control group (A,B), the dendritic morphologies of PV interneurons were impaired by exposure to sevoflurane at PD7 (C,D). (B1,B2,D1,D2) High magnification images of PV interneurons in the MOB. Compared to the control group (E,F), the dendritic morphologies of CR interneurons were not impaired by exposure to sevoflurane at PD7 (G,H). (F1,F2,H1,H2) High magnification images of CR interneurons in the MOB. Compared to the control group (I,J), the dendritic morphologies of CB interneurons were not impaired by exposure to sevoflurane at PD7 (K,L). (J1,J2,L1,L2) High magnification images of CR interneurons in the MOB. Scale bars = 100 μm in (K) (applies to A,C,E,G,I,K), 50 μm in (L) (applies to B,D,F,H,J,L), and 5 μm in (L2) (applies to B1,B2,D1,D2,F1,F2,H1,H2,J1,J2,L1,L2).
Mentions: To further evaluate the effect of sevoflurane on neuronal morphologies, immunofluorescence staining was used to display the dendritic arbor architecture of these interneurons in control and sevoflurane-exposed mice at PD42. The results showed that the PV-ir interneurons in the MOB in control mice were multipolar neurons with extensive and clear dendrite complexities with visible secondary and tertiary dendrites (Figures 4A–B2). In the sevoflurane-treated mice, the dendritic branches of PV-ir interneurons were significantly decreased, and some of these neurons had no dendrites (Figures 4D–D2). Unlike in the PV-expressing neurons, the dendrites of the CR-ir and CB-ir interneurons were not affected by sevoflurane exposure at PD7 (Figures 4E–L).

Bottom Line: Sevoflurane is widely used in adult and pediatric patients during clinical surgeries.This study detected the effect of sevoflurane exposure on the development of calcium-binding proteins-expressing interneurons in the main olfactory bulb (MOB).We exposed neonatal mice to 2% sevoflurane at two different developmental time points and found that exposing mice to sevoflurane at postnatal day (PD) 7 significantly decreased the expression of GAD67 and parvalbumin (PV) in the olfactory bulb (OB) but did not alter the expression of calretinin (CR) or calbindin D28k (CB).

View Article: PubMed Central - PubMed

Affiliation: Institute of Neuroscience and Anatomy, School of Medicine, Zhejiang University Hangzhou, China.

ABSTRACT
Sevoflurane is widely used in adult and pediatric patients during clinical surgeries. Although studies have shown that exposure to sevoflurane impairs solfactory memory after an operation, the neuropathological changes underlying this effect are not clear. This study detected the effect of sevoflurane exposure on the development of calcium-binding proteins-expressing interneurons in the main olfactory bulb (MOB). We exposed neonatal mice to 2% sevoflurane at two different developmental time points and found that exposing mice to sevoflurane at postnatal day (PD) 7 significantly decreased the expression of GAD67 and parvalbumin (PV) in the olfactory bulb (OB) but did not alter the expression of calretinin (CR) or calbindin D28k (CB). The number and dendritic morphology of PV-expressing interneurons in the MOB were impaired by exposure to sevoflurane at PD7. However, exposure to sevoflurane at PD10 had no effect on calcium-binding protein expression or the number and dendritic morphology of PV-expressing interneurons in the MOB. These results suggest that exposing neonatal mice to sevoflurane during a critical period of olfactory development affects the development of PV-expressing interneurons in the MOB.

No MeSH data available.


Related in: MedlinePlus