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Great horsetail (Equisetum telmateia Ehrh.): Active substances content and biological effects.

Radojevic ID, Stankovic MS, Stefanovic OD, Topuzovic MD, Comic LR, Ostojic AM - EXCLI J (2012)

Bottom Line: The highest phenolic content, concentrations of flavonoids and capacity to neutralize DPPH radicals were found in the acetone extract.There were statistically significant differences in activity between the extracts of E. telmateia.All the extracts showed significant antibacterial activity against G+ bacteria and weak to moderate activity against other microorganisms.

View Article: PubMed Central - PubMed

Affiliation: Department of Biology and Ecology, Faculty of Science, University of Kragujevac, Radoja Domanovica 12, 34000 Kragujevac, Republic of Serbia.

ABSTRACT
This paper deals with the antioxidant and antimicrobial activity, total phenolic content and concentrations of flavonoids of Equisetum telmateia extracts. Total phenolic content was determined with Folin-Ciocalteu reagent and it ranged between 129.0 to 262.7 mg GA/g. The concentration of flavonoids in various extracts of E. telmateia was determined using spectrophotometric method with aluminum chloride and obtained results varied from 112.6 to 199.8 mg RU/g. Antioxidant activity was monitored spectrophotometrically and expressed in terms of IC50 (µg/ml), and its values ranged from 33.4 to 982.2 µg/ml. The highest phenolic content, concentrations of flavonoids and capacity to neutralize DPPH radicals were found in the acetone extract. In vitro antimicrobial activity was determined using microdilution method. Minimum inhibitory concentration (MIC) and minimum microbicidal concentration (MMC) were also determined. Testing was performed on 22 microorganisms, including 15 strains of bacteria (standard and clinical strains) and 7 species of fungi. There were statistically significant differences in activity between the extracts of E. telmateia. Different effects were noticed against the bacteria and the methanol extract appeared to be most efficient. All the extracts showed significant antibacterial activity against G+ bacteria and weak to moderate activity against other microorganisms.

No MeSH data available.


Related in: MedlinePlus

Antifungal activities of acetone, ethyl acetate and methanolic extracts of E. telmateia against tested microorganisms based on microdilution method
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T4: Antifungal activities of acetone, ethyl acetate and methanolic extracts of E. telmateia against tested microorganisms based on microdilution method

Mentions: The in vitro results of antibacterial and antifungal activities of acetone, ethyl acetate and methanol extracts of E. telmateia are shown in Tables 3(Tab. 3) and 4(Tab. 4). For comparison, MIC and MMC values for doxycycline and fluconazole are also given in Tables 3(Tab. 3) and 4(Tab. 4). The solvent (10 % DMSO) did not inhibit the growth of the tested microorganisms.


Great horsetail (Equisetum telmateia Ehrh.): Active substances content and biological effects.

Radojevic ID, Stankovic MS, Stefanovic OD, Topuzovic MD, Comic LR, Ostojic AM - EXCLI J (2012)

Antifungal activities of acetone, ethyl acetate and methanolic extracts of E. telmateia against tested microorganisms based on microdilution method
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4920040&req=5

T4: Antifungal activities of acetone, ethyl acetate and methanolic extracts of E. telmateia against tested microorganisms based on microdilution method
Mentions: The in vitro results of antibacterial and antifungal activities of acetone, ethyl acetate and methanol extracts of E. telmateia are shown in Tables 3(Tab. 3) and 4(Tab. 4). For comparison, MIC and MMC values for doxycycline and fluconazole are also given in Tables 3(Tab. 3) and 4(Tab. 4). The solvent (10 % DMSO) did not inhibit the growth of the tested microorganisms.

Bottom Line: The highest phenolic content, concentrations of flavonoids and capacity to neutralize DPPH radicals were found in the acetone extract.There were statistically significant differences in activity between the extracts of E. telmateia.All the extracts showed significant antibacterial activity against G+ bacteria and weak to moderate activity against other microorganisms.

View Article: PubMed Central - PubMed

Affiliation: Department of Biology and Ecology, Faculty of Science, University of Kragujevac, Radoja Domanovica 12, 34000 Kragujevac, Republic of Serbia.

ABSTRACT
This paper deals with the antioxidant and antimicrobial activity, total phenolic content and concentrations of flavonoids of Equisetum telmateia extracts. Total phenolic content was determined with Folin-Ciocalteu reagent and it ranged between 129.0 to 262.7 mg GA/g. The concentration of flavonoids in various extracts of E. telmateia was determined using spectrophotometric method with aluminum chloride and obtained results varied from 112.6 to 199.8 mg RU/g. Antioxidant activity was monitored spectrophotometrically and expressed in terms of IC50 (µg/ml), and its values ranged from 33.4 to 982.2 µg/ml. The highest phenolic content, concentrations of flavonoids and capacity to neutralize DPPH radicals were found in the acetone extract. In vitro antimicrobial activity was determined using microdilution method. Minimum inhibitory concentration (MIC) and minimum microbicidal concentration (MMC) were also determined. Testing was performed on 22 microorganisms, including 15 strains of bacteria (standard and clinical strains) and 7 species of fungi. There were statistically significant differences in activity between the extracts of E. telmateia. Different effects were noticed against the bacteria and the methanol extract appeared to be most efficient. All the extracts showed significant antibacterial activity against G+ bacteria and weak to moderate activity against other microorganisms.

No MeSH data available.


Related in: MedlinePlus