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Great horsetail (Equisetum telmateia Ehrh.): Active substances content and biological effects.

Radojevic ID, Stankovic MS, Stefanovic OD, Topuzovic MD, Comic LR, Ostojic AM - EXCLI J (2012)

Bottom Line: The highest phenolic content, concentrations of flavonoids and capacity to neutralize DPPH radicals were found in the acetone extract.There were statistically significant differences in activity between the extracts of E. telmateia.All the extracts showed significant antibacterial activity against G+ bacteria and weak to moderate activity against other microorganisms.

View Article: PubMed Central - PubMed

Affiliation: Department of Biology and Ecology, Faculty of Science, University of Kragujevac, Radoja Domanovica 12, 34000 Kragujevac, Republic of Serbia.

ABSTRACT
This paper deals with the antioxidant and antimicrobial activity, total phenolic content and concentrations of flavonoids of Equisetum telmateia extracts. Total phenolic content was determined with Folin-Ciocalteu reagent and it ranged between 129.0 to 262.7 mg GA/g. The concentration of flavonoids in various extracts of E. telmateia was determined using spectrophotometric method with aluminum chloride and obtained results varied from 112.6 to 199.8 mg RU/g. Antioxidant activity was monitored spectrophotometrically and expressed in terms of IC50 (µg/ml), and its values ranged from 33.4 to 982.2 µg/ml. The highest phenolic content, concentrations of flavonoids and capacity to neutralize DPPH radicals were found in the acetone extract. In vitro antimicrobial activity was determined using microdilution method. Minimum inhibitory concentration (MIC) and minimum microbicidal concentration (MMC) were also determined. Testing was performed on 22 microorganisms, including 15 strains of bacteria (standard and clinical strains) and 7 species of fungi. There were statistically significant differences in activity between the extracts of E. telmateia. Different effects were noticed against the bacteria and the methanol extract appeared to be most efficient. All the extracts showed significant antibacterial activity against G+ bacteria and weak to moderate activity against other microorganisms.

No MeSH data available.


Related in: MedlinePlus

Antioxidant (DPPH scavenging) activity1 of investigated plant extracts and standard substance presented as IC50 values (µg/ml).
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T2: Antioxidant (DPPH scavenging) activity1 of investigated plant extracts and standard substance presented as IC50 values (µg/ml).

Mentions: The antioxidant activity of three different extracts from E. telmateia is expressed in terms of IC50 (µg/ml) values. Also, the values were obtained for chlorogenic acid as the reference substance and compared with the values of the antioxidant activity of E. telmateia. The summary of obtained IC50 values of antioxidant activity of the extracts is given in Table 2(Tab. 2). The obtained values for antioxidant activity examined by DPPH radical scavenging activity ranged from 33.4 to 982.2 µg/ml. The largest capacity in neutralization of DPPH radicals was measured in the methanol extract from E. telmateia, which neutralized 50 % of free radicals in a very small concentration (33.4 µg/ml). In measuring total phenolic content, methanolic extract showed the highest values. Based on these results, each extract of E. telmateia showed a phenol concentration-dependent scavenging effect. Numerous investigations of the antioxidant activity of plant extracts have confirmed a high linear correlation between the values of total phenolic content and antioxidant activity (Katalinić et al., 2006[7]).


Great horsetail (Equisetum telmateia Ehrh.): Active substances content and biological effects.

Radojevic ID, Stankovic MS, Stefanovic OD, Topuzovic MD, Comic LR, Ostojic AM - EXCLI J (2012)

Antioxidant (DPPH scavenging) activity1 of investigated plant extracts and standard substance presented as IC50 values (µg/ml).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4920040&req=5

T2: Antioxidant (DPPH scavenging) activity1 of investigated plant extracts and standard substance presented as IC50 values (µg/ml).
Mentions: The antioxidant activity of three different extracts from E. telmateia is expressed in terms of IC50 (µg/ml) values. Also, the values were obtained for chlorogenic acid as the reference substance and compared with the values of the antioxidant activity of E. telmateia. The summary of obtained IC50 values of antioxidant activity of the extracts is given in Table 2(Tab. 2). The obtained values for antioxidant activity examined by DPPH radical scavenging activity ranged from 33.4 to 982.2 µg/ml. The largest capacity in neutralization of DPPH radicals was measured in the methanol extract from E. telmateia, which neutralized 50 % of free radicals in a very small concentration (33.4 µg/ml). In measuring total phenolic content, methanolic extract showed the highest values. Based on these results, each extract of E. telmateia showed a phenol concentration-dependent scavenging effect. Numerous investigations of the antioxidant activity of plant extracts have confirmed a high linear correlation between the values of total phenolic content and antioxidant activity (Katalinić et al., 2006[7]).

Bottom Line: The highest phenolic content, concentrations of flavonoids and capacity to neutralize DPPH radicals were found in the acetone extract.There were statistically significant differences in activity between the extracts of E. telmateia.All the extracts showed significant antibacterial activity against G+ bacteria and weak to moderate activity against other microorganisms.

View Article: PubMed Central - PubMed

Affiliation: Department of Biology and Ecology, Faculty of Science, University of Kragujevac, Radoja Domanovica 12, 34000 Kragujevac, Republic of Serbia.

ABSTRACT
This paper deals with the antioxidant and antimicrobial activity, total phenolic content and concentrations of flavonoids of Equisetum telmateia extracts. Total phenolic content was determined with Folin-Ciocalteu reagent and it ranged between 129.0 to 262.7 mg GA/g. The concentration of flavonoids in various extracts of E. telmateia was determined using spectrophotometric method with aluminum chloride and obtained results varied from 112.6 to 199.8 mg RU/g. Antioxidant activity was monitored spectrophotometrically and expressed in terms of IC50 (µg/ml), and its values ranged from 33.4 to 982.2 µg/ml. The highest phenolic content, concentrations of flavonoids and capacity to neutralize DPPH radicals were found in the acetone extract. In vitro antimicrobial activity was determined using microdilution method. Minimum inhibitory concentration (MIC) and minimum microbicidal concentration (MMC) were also determined. Testing was performed on 22 microorganisms, including 15 strains of bacteria (standard and clinical strains) and 7 species of fungi. There were statistically significant differences in activity between the extracts of E. telmateia. Different effects were noticed against the bacteria and the methanol extract appeared to be most efficient. All the extracts showed significant antibacterial activity against G+ bacteria and weak to moderate activity against other microorganisms.

No MeSH data available.


Related in: MedlinePlus