Limits...
Isolation, partial purification and evaluation of bioactive compounds from leaves of Ageratum houstonianum.

Zeeshan M, Rizvi SM, Khan MS, Kumar A - EXCLI J (2012)

Bottom Line: The most important compounds identified in the crude extract and active bands (AB-1 and AB-2) were 6-acetyl-7-methoxy-2, 2-dimethylchromene, hexadecanoic acid and squalene, respectively.Dose-dependant increase in antioxidant potential was noticed in crude extract as well as with both active bands measured by DPPH free radicals, ion chelation and total antioxidants capacity.Our study reports various bioactive compounds in the leaves of the A. houstonianum with significant antioxidant and antibacterial potential.

View Article: PubMed Central - PubMed

Affiliation: Department of Biotechnology and Microbiology, Integral University, Lucknow-226 026, India.

ABSTRACT
The present study deals with the isolation and partial purification of bioactive compounds from the crude methanol extracts of the leaves of Ageratum houstonianum (Asteraceae). The quantification and the identification of compounds in the crude extract and active bands isolated by preparative TLC were accomplished using GC-MS analysis. The most important compounds identified in the crude extract and active bands (AB-1 and AB-2) were 6-acetyl-7-methoxy-2, 2-dimethylchromene, hexadecanoic acid and squalene, respectively. Crude extract and active bands (AB-1 and AB-2) were investigated for their antibacterial activity against Staphylococcus epidermidis, Staphylococcus aureus, Bacillus subtilis, Bacillus cereus, Escherichia coli, Enterobacter aerogenes, Klebsiella pneumoniae, and Pseudomonas aeruginosa. The crude extract, AB-1 and AB-2 showed maximum zone of inhibition (10-13 mm) against Staphylococcus epidermidis, however, the antibacterial potential of active bands was slightly higher as compared to the crude extract. Dose-dependant increase in antioxidant potential was noticed in crude extract as well as with both active bands measured by DPPH free radicals, ion chelation and total antioxidants capacity. Our study reports various bioactive compounds in the leaves of the A. houstonianum with significant antioxidant and antibacterial potential.

No MeSH data available.


Related in: MedlinePlus

Bioactive compounds identified in the active band (AB-1) isolated from methanolic crude extract of the leaves of A. houstonianum by GC-MS
© Copyright Policy - open-access
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4920037&req=5

T3: Bioactive compounds identified in the active band (AB-1) isolated from methanolic crude extract of the leaves of A. houstonianum by GC-MS

Mentions: The present study tried to evaluate the bioactive potential of partially purified compounds isolated by preparative TLC from the leaves of Ageratum sp. and their characterization by GC-MS analysis. The methanol crude extract of leaves of Ageratum houstonianum showed antibacterial activity against Staphylococcus epidermidis, Staphylococcus aureus, Bacillus subtilis, Bacillus cereus, and Enterobacter aerogenes whereas, no activity was noticed against Escherichia coli, Klebsiella pneumoniae, and Pseudomonas aeruginosa (Table 1(Tab. 1)). The disc (400 μg) of crude extract exhibited maximum zone of inhibition (10 mm) against S. epidermidis followed by B. cereus (9 mm), E. aerogenes (9 mm), B. subtilis (8 mm) and S. aureus (8 mm). Based on these results methanolic crude extract of leaves was used to detect the bioactive compounds through TLC. The TLC results indicate the presence of seven bands under ultra violet light (365 nm) illumination (Figure 1(Fig. 1)). Out of these, two active bands (AB-1) and (AB-2) with Rf value - 0.20 and 0.48, respectively were selected to evaluate the bioactive potential based on their good antibacterial activity against S. epidermidis (data not shown). Further, through preparative TLC the compounds of both active bands were pooled separately and subjected to antibacterial, antioxidant and G-MS analysis. The yields of compounds were 25 µg/mg and 62.5 µg/mg of crude extract for AB-1 and AB-2, respectively. The GC-MS analysis of crude and active band as shown in Figures 2-4(Fig. 2)(Fig. 3)(Fig. 4) indicates the presence of different components. The major components present in the methanolic crude extract of leaves of A. houstonianum were diazoprogesterone (RT: 20.88), 6-acetyl-7-methoxy-2,2-dimethylchromene (RT: 22.30), 9,12-octadecadienoate (RT: 32.18), stigmast-5-en-3-ol (RT: 52.15) and 3,7-dimethyl-2,6-octadienyl acetate (RT:54.19) (Table 2(Tab. 2)). Seven components were identified in active band (AB-1), out of which hexadecanoic acid (RT: 27.94) and 3'-Acetyllycopsamine (RT: 29.40) were the major compounds (Table 3(Tab. 3)). Six components were detected in the active band (AB-2), however, squalene (RT: 31.83) and phenol (RT: 31.96) were the major components (Table 4(Tab. 4)).


Isolation, partial purification and evaluation of bioactive compounds from leaves of Ageratum houstonianum.

Zeeshan M, Rizvi SM, Khan MS, Kumar A - EXCLI J (2012)

Bioactive compounds identified in the active band (AB-1) isolated from methanolic crude extract of the leaves of A. houstonianum by GC-MS
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4920037&req=5

T3: Bioactive compounds identified in the active band (AB-1) isolated from methanolic crude extract of the leaves of A. houstonianum by GC-MS
Mentions: The present study tried to evaluate the bioactive potential of partially purified compounds isolated by preparative TLC from the leaves of Ageratum sp. and their characterization by GC-MS analysis. The methanol crude extract of leaves of Ageratum houstonianum showed antibacterial activity against Staphylococcus epidermidis, Staphylococcus aureus, Bacillus subtilis, Bacillus cereus, and Enterobacter aerogenes whereas, no activity was noticed against Escherichia coli, Klebsiella pneumoniae, and Pseudomonas aeruginosa (Table 1(Tab. 1)). The disc (400 μg) of crude extract exhibited maximum zone of inhibition (10 mm) against S. epidermidis followed by B. cereus (9 mm), E. aerogenes (9 mm), B. subtilis (8 mm) and S. aureus (8 mm). Based on these results methanolic crude extract of leaves was used to detect the bioactive compounds through TLC. The TLC results indicate the presence of seven bands under ultra violet light (365 nm) illumination (Figure 1(Fig. 1)). Out of these, two active bands (AB-1) and (AB-2) with Rf value - 0.20 and 0.48, respectively were selected to evaluate the bioactive potential based on their good antibacterial activity against S. epidermidis (data not shown). Further, through preparative TLC the compounds of both active bands were pooled separately and subjected to antibacterial, antioxidant and G-MS analysis. The yields of compounds were 25 µg/mg and 62.5 µg/mg of crude extract for AB-1 and AB-2, respectively. The GC-MS analysis of crude and active band as shown in Figures 2-4(Fig. 2)(Fig. 3)(Fig. 4) indicates the presence of different components. The major components present in the methanolic crude extract of leaves of A. houstonianum were diazoprogesterone (RT: 20.88), 6-acetyl-7-methoxy-2,2-dimethylchromene (RT: 22.30), 9,12-octadecadienoate (RT: 32.18), stigmast-5-en-3-ol (RT: 52.15) and 3,7-dimethyl-2,6-octadienyl acetate (RT:54.19) (Table 2(Tab. 2)). Seven components were identified in active band (AB-1), out of which hexadecanoic acid (RT: 27.94) and 3'-Acetyllycopsamine (RT: 29.40) were the major compounds (Table 3(Tab. 3)). Six components were detected in the active band (AB-2), however, squalene (RT: 31.83) and phenol (RT: 31.96) were the major components (Table 4(Tab. 4)).

Bottom Line: The most important compounds identified in the crude extract and active bands (AB-1 and AB-2) were 6-acetyl-7-methoxy-2, 2-dimethylchromene, hexadecanoic acid and squalene, respectively.Dose-dependant increase in antioxidant potential was noticed in crude extract as well as with both active bands measured by DPPH free radicals, ion chelation and total antioxidants capacity.Our study reports various bioactive compounds in the leaves of the A. houstonianum with significant antioxidant and antibacterial potential.

View Article: PubMed Central - PubMed

Affiliation: Department of Biotechnology and Microbiology, Integral University, Lucknow-226 026, India.

ABSTRACT
The present study deals with the isolation and partial purification of bioactive compounds from the crude methanol extracts of the leaves of Ageratum houstonianum (Asteraceae). The quantification and the identification of compounds in the crude extract and active bands isolated by preparative TLC were accomplished using GC-MS analysis. The most important compounds identified in the crude extract and active bands (AB-1 and AB-2) were 6-acetyl-7-methoxy-2, 2-dimethylchromene, hexadecanoic acid and squalene, respectively. Crude extract and active bands (AB-1 and AB-2) were investigated for their antibacterial activity against Staphylococcus epidermidis, Staphylococcus aureus, Bacillus subtilis, Bacillus cereus, Escherichia coli, Enterobacter aerogenes, Klebsiella pneumoniae, and Pseudomonas aeruginosa. The crude extract, AB-1 and AB-2 showed maximum zone of inhibition (10-13 mm) against Staphylococcus epidermidis, however, the antibacterial potential of active bands was slightly higher as compared to the crude extract. Dose-dependant increase in antioxidant potential was noticed in crude extract as well as with both active bands measured by DPPH free radicals, ion chelation and total antioxidants capacity. Our study reports various bioactive compounds in the leaves of the A. houstonianum with significant antioxidant and antibacterial potential.

No MeSH data available.


Related in: MedlinePlus