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Isolation, partial purification and evaluation of bioactive compounds from leaves of Ageratum houstonianum.

Zeeshan M, Rizvi SM, Khan MS, Kumar A - EXCLI J (2012)

Bottom Line: The most important compounds identified in the crude extract and active bands (AB-1 and AB-2) were 6-acetyl-7-methoxy-2, 2-dimethylchromene, hexadecanoic acid and squalene, respectively.Dose-dependant increase in antioxidant potential was noticed in crude extract as well as with both active bands measured by DPPH free radicals, ion chelation and total antioxidants capacity.Our study reports various bioactive compounds in the leaves of the A. houstonianum with significant antioxidant and antibacterial potential.

View Article: PubMed Central - PubMed

Affiliation: Department of Biotechnology and Microbiology, Integral University, Lucknow-226 026, India.

ABSTRACT
The present study deals with the isolation and partial purification of bioactive compounds from the crude methanol extracts of the leaves of Ageratum houstonianum (Asteraceae). The quantification and the identification of compounds in the crude extract and active bands isolated by preparative TLC were accomplished using GC-MS analysis. The most important compounds identified in the crude extract and active bands (AB-1 and AB-2) were 6-acetyl-7-methoxy-2, 2-dimethylchromene, hexadecanoic acid and squalene, respectively. Crude extract and active bands (AB-1 and AB-2) were investigated for their antibacterial activity against Staphylococcus epidermidis, Staphylococcus aureus, Bacillus subtilis, Bacillus cereus, Escherichia coli, Enterobacter aerogenes, Klebsiella pneumoniae, and Pseudomonas aeruginosa. The crude extract, AB-1 and AB-2 showed maximum zone of inhibition (10-13 mm) against Staphylococcus epidermidis, however, the antibacterial potential of active bands was slightly higher as compared to the crude extract. Dose-dependant increase in antioxidant potential was noticed in crude extract as well as with both active bands measured by DPPH free radicals, ion chelation and total antioxidants capacity. Our study reports various bioactive compounds in the leaves of the A. houstonianum with significant antioxidant and antibacterial potential.

No MeSH data available.


Related in: MedlinePlus

Total antioxidant activity of crude extract and active bands (AB-1 and AB-2) isolated through TLC from leaves of A. houstonianum *expressed in terms of ascorbic acid equivalent (µg/ml). Values are mean ± SE of three replicates
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Figure 6: Total antioxidant activity of crude extract and active bands (AB-1 and AB-2) isolated through TLC from leaves of A. houstonianum *expressed in terms of ascorbic acid equivalent (µg/ml). Values are mean ± SE of three replicates

Mentions: For evaluation of antioxidant properties of crude extract and TLC eluted compounds of Ageratum sp. three assays have been used: DPPH radical scavenging, Total antioxidant and Ion chelation assays and the results are shown in Figures 5(Fig. 5), 6(Fig. 6) and 7(Fig. 7). DPPH was reduced with the addition of crude and active bands in a concentration dependent manner (Figure 5(Fig. 5)). Crude extract was the most effective radical scavenger with the inhibition of 55.8 % at 0.5 mg/ml while the scavenging activity of active bands AB-1 and AB-2 was lower as 42.3 % and 39.05 % inhibition was noticed at 0.5 mg/ml, respectively. The results of total antioxidant capacities of crude and partially purifed compounds based on the formation of phosphomolybdenum complex are shown in Figure 6(Fig. 6). In this assay, crude extract possessed more antioxidant potential with 9.4 µg equivalent of ascorbic acid at 8 µg/ml concentration than the partially purified compounds which showed only 1 and 2.5 µg equivalent of ascorbic acid at 8 µg/ml concentration. The ion chelation activity was studied by interaction of crude or partially purifed compounds with ferrous-o-phenanthroline complex (Figure 7(Fig. 7)). Similar to this the crude extract has more ion chelation capacity than the active bands.


Isolation, partial purification and evaluation of bioactive compounds from leaves of Ageratum houstonianum.

Zeeshan M, Rizvi SM, Khan MS, Kumar A - EXCLI J (2012)

Total antioxidant activity of crude extract and active bands (AB-1 and AB-2) isolated through TLC from leaves of A. houstonianum *expressed in terms of ascorbic acid equivalent (µg/ml). Values are mean ± SE of three replicates
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4920037&req=5

Figure 6: Total antioxidant activity of crude extract and active bands (AB-1 and AB-2) isolated through TLC from leaves of A. houstonianum *expressed in terms of ascorbic acid equivalent (µg/ml). Values are mean ± SE of three replicates
Mentions: For evaluation of antioxidant properties of crude extract and TLC eluted compounds of Ageratum sp. three assays have been used: DPPH radical scavenging, Total antioxidant and Ion chelation assays and the results are shown in Figures 5(Fig. 5), 6(Fig. 6) and 7(Fig. 7). DPPH was reduced with the addition of crude and active bands in a concentration dependent manner (Figure 5(Fig. 5)). Crude extract was the most effective radical scavenger with the inhibition of 55.8 % at 0.5 mg/ml while the scavenging activity of active bands AB-1 and AB-2 was lower as 42.3 % and 39.05 % inhibition was noticed at 0.5 mg/ml, respectively. The results of total antioxidant capacities of crude and partially purifed compounds based on the formation of phosphomolybdenum complex are shown in Figure 6(Fig. 6). In this assay, crude extract possessed more antioxidant potential with 9.4 µg equivalent of ascorbic acid at 8 µg/ml concentration than the partially purified compounds which showed only 1 and 2.5 µg equivalent of ascorbic acid at 8 µg/ml concentration. The ion chelation activity was studied by interaction of crude or partially purifed compounds with ferrous-o-phenanthroline complex (Figure 7(Fig. 7)). Similar to this the crude extract has more ion chelation capacity than the active bands.

Bottom Line: The most important compounds identified in the crude extract and active bands (AB-1 and AB-2) were 6-acetyl-7-methoxy-2, 2-dimethylchromene, hexadecanoic acid and squalene, respectively.Dose-dependant increase in antioxidant potential was noticed in crude extract as well as with both active bands measured by DPPH free radicals, ion chelation and total antioxidants capacity.Our study reports various bioactive compounds in the leaves of the A. houstonianum with significant antioxidant and antibacterial potential.

View Article: PubMed Central - PubMed

Affiliation: Department of Biotechnology and Microbiology, Integral University, Lucknow-226 026, India.

ABSTRACT
The present study deals with the isolation and partial purification of bioactive compounds from the crude methanol extracts of the leaves of Ageratum houstonianum (Asteraceae). The quantification and the identification of compounds in the crude extract and active bands isolated by preparative TLC were accomplished using GC-MS analysis. The most important compounds identified in the crude extract and active bands (AB-1 and AB-2) were 6-acetyl-7-methoxy-2, 2-dimethylchromene, hexadecanoic acid and squalene, respectively. Crude extract and active bands (AB-1 and AB-2) were investigated for their antibacterial activity against Staphylococcus epidermidis, Staphylococcus aureus, Bacillus subtilis, Bacillus cereus, Escherichia coli, Enterobacter aerogenes, Klebsiella pneumoniae, and Pseudomonas aeruginosa. The crude extract, AB-1 and AB-2 showed maximum zone of inhibition (10-13 mm) against Staphylococcus epidermidis, however, the antibacterial potential of active bands was slightly higher as compared to the crude extract. Dose-dependant increase in antioxidant potential was noticed in crude extract as well as with both active bands measured by DPPH free radicals, ion chelation and total antioxidants capacity. Our study reports various bioactive compounds in the leaves of the A. houstonianum with significant antioxidant and antibacterial potential.

No MeSH data available.


Related in: MedlinePlus