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Antiviral factors and type I/III interferon expression associated with regulatory factors in the oral epithelial cells from HIV-1-serodiscordant couples.

Cervantes CA, Oliveira LM, Manfrere KC, Lima JF, Pereira NZ, Duarte AJ, Sato MN - Sci Rep (2016)

Bottom Line: Innate and adaptive immunity, as well as genetic factors, provide ESNs with important advantages that allow for low infection susceptibility.Our findings revealed that ESNs did not induce the expression of antiviral factors (APOBEC-3G, TRIM5-α, SAMDH1, STING, TBk1) or regulatory factors (Trex, Foxo3, Socs3, IL-10) in PBMCs, unlike their HIV-1-infected partners.These findings revealed evidence of antiviral factors, type I/III interferon and regulatory factor expression only in the oral mucosal compartment of ESNs, while HIV-1-infected partners systemically and oral mucosal expressed the antiviral profile.

View Article: PubMed Central - PubMed

Affiliation: Laboratory of Dermatology and Immunodeficiencies, LIM-56, Department of Dermatology, Institute of Tropical Medicine, University of São Paulo, São Paulo, Brazil.

ABSTRACT
Individuals who remain HIV-seronegative despite repeated unprotected exposure to the virus are defined as exposed seronegative (ESN) individuals. Innate and adaptive immunity, as well as genetic factors, provide ESNs with important advantages that allow for low infection susceptibility. The majority of HIV-1-infected individuals undergo antiretroviral therapy, which can decrease the level of HIV-1 exposure in ESNs. We analyzed type I interferon (IFN)-related antiviral and regulatory factors in peripheral blood mononuclear cells (PBMCs) and oral epithelial cells from serodiscordant couples. Our findings revealed that ESNs did not induce the expression of antiviral factors (APOBEC-3G, TRIM5-α, SAMDH1, STING, TBk1) or regulatory factors (Trex, Foxo3, Socs3, IL-10) in PBMCs, unlike their HIV-1-infected partners. In contrast, ESNs upregulated APOBEC-3G and type I/III IFNs (IFNs-α,-β/-λ) in oral mucosal epithelial cells similar to their HIV-infected partners. The serodiscordant groups exhibited an increased expression of type I IFN-induced regulators, such as Trex and Foxo3, in oral epithelial cells. TLR7, TLR8 and TLR9 were expressed in oral epithelial cells of both ESNs and HIV-1-infected subjects. These findings revealed evidence of antiviral factors, type I/III interferon and regulatory factor expression only in the oral mucosal compartment of ESNs, while HIV-1-infected partners systemically and oral mucosal expressed the antiviral profile.

No MeSH data available.


Related in: MedlinePlus

Increased TLR7, TLR8 and TLR9 expression in the mucosal oral epithelial cells from serodiscordant couples.The antiviral mRNA expression levels in cells from buccal washes from healthy controls (HCs, n = 5–10), exposed seronegative individuals (ESNs, n = 13) and HIV-1-infected partners (n = 13) were evaluated by real-time PCR. The data represent the median values. *p ≤ 0.05.
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f4: Increased TLR7, TLR8 and TLR9 expression in the mucosal oral epithelial cells from serodiscordant couples.The antiviral mRNA expression levels in cells from buccal washes from healthy controls (HCs, n = 5–10), exposed seronegative individuals (ESNs, n = 13) and HIV-1-infected partners (n = 13) were evaluated by real-time PCR. The data represent the median values. *p ≤ 0.05.

Mentions: Because we identified a relevant antiviral expression pattern, we evaluated the expression of TLRs, such as TLR3, TLR7, TLR8, and TLR9, which are associated with the antiviral response. As shown in Fig. 4, TLR7, TLR8 and TLR9 were expressed at detectable levels in epithelial cells of serodiscordant couples, and TLR9 expression was increased compared to HC individuals. We did not detect TLR3 expression in oral epithelial cells.


Antiviral factors and type I/III interferon expression associated with regulatory factors in the oral epithelial cells from HIV-1-serodiscordant couples.

Cervantes CA, Oliveira LM, Manfrere KC, Lima JF, Pereira NZ, Duarte AJ, Sato MN - Sci Rep (2016)

Increased TLR7, TLR8 and TLR9 expression in the mucosal oral epithelial cells from serodiscordant couples.The antiviral mRNA expression levels in cells from buccal washes from healthy controls (HCs, n = 5–10), exposed seronegative individuals (ESNs, n = 13) and HIV-1-infected partners (n = 13) were evaluated by real-time PCR. The data represent the median values. *p ≤ 0.05.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4863167&req=5

f4: Increased TLR7, TLR8 and TLR9 expression in the mucosal oral epithelial cells from serodiscordant couples.The antiviral mRNA expression levels in cells from buccal washes from healthy controls (HCs, n = 5–10), exposed seronegative individuals (ESNs, n = 13) and HIV-1-infected partners (n = 13) were evaluated by real-time PCR. The data represent the median values. *p ≤ 0.05.
Mentions: Because we identified a relevant antiviral expression pattern, we evaluated the expression of TLRs, such as TLR3, TLR7, TLR8, and TLR9, which are associated with the antiviral response. As shown in Fig. 4, TLR7, TLR8 and TLR9 were expressed at detectable levels in epithelial cells of serodiscordant couples, and TLR9 expression was increased compared to HC individuals. We did not detect TLR3 expression in oral epithelial cells.

Bottom Line: Innate and adaptive immunity, as well as genetic factors, provide ESNs with important advantages that allow for low infection susceptibility.Our findings revealed that ESNs did not induce the expression of antiviral factors (APOBEC-3G, TRIM5-α, SAMDH1, STING, TBk1) or regulatory factors (Trex, Foxo3, Socs3, IL-10) in PBMCs, unlike their HIV-1-infected partners.These findings revealed evidence of antiviral factors, type I/III interferon and regulatory factor expression only in the oral mucosal compartment of ESNs, while HIV-1-infected partners systemically and oral mucosal expressed the antiviral profile.

View Article: PubMed Central - PubMed

Affiliation: Laboratory of Dermatology and Immunodeficiencies, LIM-56, Department of Dermatology, Institute of Tropical Medicine, University of São Paulo, São Paulo, Brazil.

ABSTRACT
Individuals who remain HIV-seronegative despite repeated unprotected exposure to the virus are defined as exposed seronegative (ESN) individuals. Innate and adaptive immunity, as well as genetic factors, provide ESNs with important advantages that allow for low infection susceptibility. The majority of HIV-1-infected individuals undergo antiretroviral therapy, which can decrease the level of HIV-1 exposure in ESNs. We analyzed type I interferon (IFN)-related antiviral and regulatory factors in peripheral blood mononuclear cells (PBMCs) and oral epithelial cells from serodiscordant couples. Our findings revealed that ESNs did not induce the expression of antiviral factors (APOBEC-3G, TRIM5-α, SAMDH1, STING, TBk1) or regulatory factors (Trex, Foxo3, Socs3, IL-10) in PBMCs, unlike their HIV-1-infected partners. In contrast, ESNs upregulated APOBEC-3G and type I/III IFNs (IFNs-α,-β/-λ) in oral mucosal epithelial cells similar to their HIV-infected partners. The serodiscordant groups exhibited an increased expression of type I IFN-induced regulators, such as Trex and Foxo3, in oral epithelial cells. TLR7, TLR8 and TLR9 were expressed in oral epithelial cells of both ESNs and HIV-1-infected subjects. These findings revealed evidence of antiviral factors, type I/III interferon and regulatory factor expression only in the oral mucosal compartment of ESNs, while HIV-1-infected partners systemically and oral mucosal expressed the antiviral profile.

No MeSH data available.


Related in: MedlinePlus