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Comparison of the Biological Characteristics of Mesenchymal Stem Cells Derived from Bone Marrow and Skin.

Liu R, Chang W, Wei H, Zhang K - Stem Cells Int (2016)

Bottom Line: The results showed that the MSCs from both sources had similar cell morphologies, surface markers, and differentiation capacities.However, the two cell types exhibited major differences in growth characteristics; the primary culture time of BMMSCs was significantly shorter than that of SMSCs, whereas the growth rate of BMMSCs was lower than that of SMSCs after passaging.Our findings provide insights into the different biological functions of both cell types.

View Article: PubMed Central - PubMed

Affiliation: Institute of Dermatology, Taiyuan City Centre Hospital, Shanxi Provincial Key Laboratory of Immunological Skin Diseases, No. 1 Dong San Dao Xiang, Taiyuan, Shanxi 030009, China.

ABSTRACT
Mesenchymal stem cells (MSCs) exhibit high proliferation and self-renewal capabilities and are critical for tissue repair and regeneration during ontogenesis. They also play a role in immunomodulation. MSCs can be isolated from a variety of tissues and have many potential applications in the clinical setting. However, MSCs of different origins may possess different biological characteristics. In this study, we performed a comprehensive comparison of MSCs isolated from bone marrow and skin (BMMSCs and SMSCs, resp.), including analysis of the skin sampling area, separation method, culture conditions, primary and passage culture times, cell surface markers, multipotency, cytokine secretion, gene expression, and fibroblast-like features. The results showed that the MSCs from both sources had similar cell morphologies, surface markers, and differentiation capacities. However, the two cell types exhibited major differences in growth characteristics; the primary culture time of BMMSCs was significantly shorter than that of SMSCs, whereas the growth rate of BMMSCs was lower than that of SMSCs after passaging. Moreover, differences in gene expression and cytokine secretion profiles were observed. For example, secretion of proliferative cytokines was significantly higher for SMSCs than for BMMSCs. Our findings provide insights into the different biological functions of both cell types.

No MeSH data available.


Related in: MedlinePlus

Morphological characteristics of bone marrow mesenchymal stem cells (BMMSCs) and skin mesenchymal stem cells (SMSCs). (a) BMMSCs cultured for 7 days. (b) BMMSCs cultured for 16 days. (c) SMSCs cultured for 16 days. (d) SMSCs cultured for 29 days. Scale bar: 10 μm.
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fig1: Morphological characteristics of bone marrow mesenchymal stem cells (BMMSCs) and skin mesenchymal stem cells (SMSCs). (a) BMMSCs cultured for 7 days. (b) BMMSCs cultured for 16 days. (c) SMSCs cultured for 16 days. (d) SMSCs cultured for 29 days. Scale bar: 10 μm.

Mentions: The cell morphologies of BMMSCs and SMSCs were similar. Isolated BMMSCs attached to the bottoms of the plates after incubation for 24 h. On days 7–10, the cells showed obvious enlargement and proliferation, forming small colonies with several to tens of fusocellular, triangular, and polygonal cells. The cells displayed typical fibroblast morphology with multilayered flat cell bodies having short cell processes connected to adjacent cells (Figure 1(a)). At approximately day 16, the cells reached 90% confluence (Figure 1(b)). When treated with trypsin, they became round; after reattachment to the plate and incubation for 24 h, the cell morphology reverted to the primary BMMSC shape. The cells reached 90% confluence after incubation for an average of 12 days.


Comparison of the Biological Characteristics of Mesenchymal Stem Cells Derived from Bone Marrow and Skin.

Liu R, Chang W, Wei H, Zhang K - Stem Cells Int (2016)

Morphological characteristics of bone marrow mesenchymal stem cells (BMMSCs) and skin mesenchymal stem cells (SMSCs). (a) BMMSCs cultured for 7 days. (b) BMMSCs cultured for 16 days. (c) SMSCs cultured for 16 days. (d) SMSCs cultured for 29 days. Scale bar: 10 μm.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4863123&req=5

fig1: Morphological characteristics of bone marrow mesenchymal stem cells (BMMSCs) and skin mesenchymal stem cells (SMSCs). (a) BMMSCs cultured for 7 days. (b) BMMSCs cultured for 16 days. (c) SMSCs cultured for 16 days. (d) SMSCs cultured for 29 days. Scale bar: 10 μm.
Mentions: The cell morphologies of BMMSCs and SMSCs were similar. Isolated BMMSCs attached to the bottoms of the plates after incubation for 24 h. On days 7–10, the cells showed obvious enlargement and proliferation, forming small colonies with several to tens of fusocellular, triangular, and polygonal cells. The cells displayed typical fibroblast morphology with multilayered flat cell bodies having short cell processes connected to adjacent cells (Figure 1(a)). At approximately day 16, the cells reached 90% confluence (Figure 1(b)). When treated with trypsin, they became round; after reattachment to the plate and incubation for 24 h, the cell morphology reverted to the primary BMMSC shape. The cells reached 90% confluence after incubation for an average of 12 days.

Bottom Line: The results showed that the MSCs from both sources had similar cell morphologies, surface markers, and differentiation capacities.However, the two cell types exhibited major differences in growth characteristics; the primary culture time of BMMSCs was significantly shorter than that of SMSCs, whereas the growth rate of BMMSCs was lower than that of SMSCs after passaging.Our findings provide insights into the different biological functions of both cell types.

View Article: PubMed Central - PubMed

Affiliation: Institute of Dermatology, Taiyuan City Centre Hospital, Shanxi Provincial Key Laboratory of Immunological Skin Diseases, No. 1 Dong San Dao Xiang, Taiyuan, Shanxi 030009, China.

ABSTRACT
Mesenchymal stem cells (MSCs) exhibit high proliferation and self-renewal capabilities and are critical for tissue repair and regeneration during ontogenesis. They also play a role in immunomodulation. MSCs can be isolated from a variety of tissues and have many potential applications in the clinical setting. However, MSCs of different origins may possess different biological characteristics. In this study, we performed a comprehensive comparison of MSCs isolated from bone marrow and skin (BMMSCs and SMSCs, resp.), including analysis of the skin sampling area, separation method, culture conditions, primary and passage culture times, cell surface markers, multipotency, cytokine secretion, gene expression, and fibroblast-like features. The results showed that the MSCs from both sources had similar cell morphologies, surface markers, and differentiation capacities. However, the two cell types exhibited major differences in growth characteristics; the primary culture time of BMMSCs was significantly shorter than that of SMSCs, whereas the growth rate of BMMSCs was lower than that of SMSCs after passaging. Moreover, differences in gene expression and cytokine secretion profiles were observed. For example, secretion of proliferative cytokines was significantly higher for SMSCs than for BMMSCs. Our findings provide insights into the different biological functions of both cell types.

No MeSH data available.


Related in: MedlinePlus